LTK sirna

LTK siRNA (Mouse)

Cat. Num. AAA8238303

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LTK; LTK siRNA (Mouse); Leukocyte tyrosine kinase receptor; LTK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LTK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 888

• Applicable Applications for LTK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LTK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LTK sirna

siRNA to inhibit LTK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 159032530
• NCBI GeneID: 17005
• NCBI Accession #: NP_976220.2
• NCBI GenBank Nucleotide #: NM_203345.2
• UniProt Accession #: P08923; A2AQ22
• Molecular Weight: 88,897 Da
• NCBI Official Full Name: leukocyte tyrosine kinase receptor isoform D
• NCBI Official Synonym Full Names: leukocyte tyrosine kinase
• NCBI Official Symbol: Ltk
• NCBI Protein Information: leukocyte tyrosine kinase receptor
• UniProt Protein Name: Leukocyte tyrosine kinase receptor
• Protein Family: Leukocyte tyrosine kinase receptor
• UniProt Gene Name: Ltk
• UniProt Entry Name: LTK_MOUSE

NCBI Description

The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]

Uniprot Description

LTK: Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. Homodimer when bound to ligand (Probable). Part a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2. Expressed in non-hematopoietic cell lines and T- and B-cell lines. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Alk family

Cellular Component: membrane; endoplasmic reticulum; plasma membrane; integral to membrane; intracellular

Molecular Function: transferase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; phosphoinositide 3-kinase cascade; signal transduction; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis