Rabbit anti-Mouse Ltk Polyclonal Antibody | anti-Ltk antibody

Ltk, CT (Ltk, Leukocyte tyrosine kinase receptor) (PE)

Cat. Num. AAA6317559

• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ltk; Polyclonal Antibody; CT (Ltk; Leukocyte tyrosine kinase receptor) (PE); Leukocyte tyrosine kinase receptor; anti-Ltk antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Ltk antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Ltk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-822 amino acids from the C-terminal region of human Mouse Ltk.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Ltk Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Ltk antibody detected the Ltk protein (arrow).)

Related Product Information for anti-Ltk antibody

The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons.

Product Categories/Family for anti-Ltk antibody

Antibodies; Protein Kinases

References

Li, J., et al. J. Biol. Chem. 283(49):34260-34272(2008). Yu, X., et al. J. Immunol. 177(10):7042-7049(2006). Li, N., et al. Hum. Mol. Genet. 13(2):171-179(2004). Thut, C.J., et al. Dev. Biol. 231(1):63-76(2001). Robinson, D.R., et al. Oncogene 19(49):5548-5557(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 159032530
• NCBI GeneID: 17005
• NCBI Accession #: NP_976220.2; NP_996825.2; NP_996824.1
• NCBI GenBank Nucleotide #: NM_203345.2
• UniProt Accession #: P08923; A2AQ22
• Molecular Weight: 88,897 Da
• NCBI Official Full Name: leukocyte tyrosine kinase receptor isoform D
• NCBI Official Synonym Full Names: leukocyte tyrosine kinase
• NCBI Official Symbol: Ltk
• NCBI Protein Information: leukocyte tyrosine kinase receptor
• UniProt Protein Name: Leukocyte tyrosine kinase receptor
• Protein Family: Leukocyte tyrosine kinase receptor
• UniProt Gene Name: Ltk
• UniProt Entry Name: LTK_MOUSE

NCBI Description

The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]

Uniprot Description

LTK: Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. Homodimer when bound to ligand (Probable). Part a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2. Expressed in non-hematopoietic cell lines and T- and B-cell lines. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Alk family

Cellular Component: endoplasmic reticulum; integral to membrane; intracellular; membrane; plasma membrane

Molecular Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell proliferation; negative regulation of apoptosis; phosphoinositide 3-kinase cascade; phosphorylation; protein amino acid phosphorylation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway