Western Blot (WB)
(Anti-LIFR rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDaObserved band size:190 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Rat LIFR Polyclonal Antibody | anti-LIFR antibody
Anti-LIFR Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
LIFR; Polyclonal Antibody; Anti-LIFR Antibody; Rabbit Polyclonal; LIFR/CD118 Antibody; Rabbit PAb; Antigen Affinity Purified; leukemia inhibitory factor receptor alpha; anti-LIFR antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rat LIFR/CD118
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LIFR antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Rat LIFR/CD118 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Rat LIFR/CD118. LIFR/CD118 specific IgG was purified by Rat LIFR/CD118 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-LIFR rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDaObserved band size:190 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-LIFR rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:120 kDaObserved band size:190 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-LIFR antibody
LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.
References
Gearing, D.P. et al., 1991, EMBO J. 10 (10): 2839-2848. Gearing, D.P. et al., 1992, New Biol. 4 (1): 61-65. Mosley, B. et al., 1996, J. Biol. Chem. 271 (51): 32635-32643. Timmermann, A. et al., 2002, Eur. J. Biochem. 269 (11): 2716-2726. Lass, A. et al., 2002, Fertil. Steril. 76 (6): 1091-1096.
NCBI and Uniprot Product Information
UniProt Accession #
UniProt Protein Name
Leukemia inhibitory factor receptor
Protein Family
UniProt Gene Name
Lifr
Similar Products
Product Notes
The LIFR lifr (Catalog #AAA8112941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIFR Antibody, Rabbit Polyclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LIFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the LIFR lifr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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