Western Blot (WB)
(Western Blot; Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat ITSN1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Rabbit anti-Rat Intersectin 1 Polyclonal Antibody | anti-ITSN1 antibody
Intersectin 1 (ITSN1) Polyclonal Antibody
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 8h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat ITSN1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Rat Serum; Primary Ab: 1ug/ml Rabbit Anti-Rat ITSN1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Recombinant ITSN1, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant ITSN1, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 10ug/ml Rabbit Anti-Rat ITSN1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 10ug/ml Rabbit Anti-Rat ITSN1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on formalin fixed paraffin- embedded Kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on formalin fixed paraffin- embedded Kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue)
NCBI and Uniprot Product Information
NCBI Description
binds to dynamin and SNAP-25; may be involved in exocytosis [RGD, Feb 2006]
Uniprot Description
ITSN1: Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Calcium-binding; GEF; GEF, Rac/Rho
Chromosomal Location of Human Ortholog: 11q11
Cellular Component: cell junction; clathrin-coated pit; endocytic vesicle; lamellipodium; plasma membrane; terminal bouton
Molecular Function: calcium ion binding; kinase activator activity; protein binding; protein complex scaffold activity; Rho guanyl-nucleotide exchange factor activity
Biological Process: endocytosis; exocytosis; negative regulation of neuron apoptosis; positive regulation of GTPase activity; positive regulation of phosphorylation; positive regulation of protein kinase B signaling; regulation of Rho protein signal transduction; small GTPase mediated signal transduction
Research Articles on ITSN1
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Product Notes
The ITSN1 itsn1 (Catalog #AAA2032783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Intersectin 1 (ITSN1) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Intersectin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ITSN1 itsn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Intersectin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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