FCM/FACS (Flow Cytometry)
(B3GALT5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human B3GALT5 Polyclonal Antibody | anti-B3GALT5 antibody
B3GALT5 Antibody (N-term)
Gene Names
B3GALT5; B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
B3GALT5, Antibody; B3GALT5 Antibody (N-term); Beta-1,3-galactosyltransferase 5; Beta-1,3-GalTase 5; Beta3Gal-T5; Beta3GalT5; b3Gal-T5; 241-; Beta-3-Gx-T5; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5; B3GALT5; anti-B3GALT5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This B3GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human B3GALT5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
57-84
Sequence Length
310
Applicable Applications for anti-B3GALT5 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(B3GALT5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(B3GALT5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(B3GALT5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the B3GALT5 antibody detected the B3GALT5 protein (arrow).)
WB (Western Blot)
(B3GALT5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the B3GALT5 antibody detected the B3GALT5 protein (arrow).)
Related Product Information for anti-B3GALT5 antibody
This gene is a member of the
beta-1,3-galactosyltransferase (beta3GalT) gene family. This family
encodes type II membrane-bound glycoproteins with diverse enzymatic
functions using different donor substrates (UDP-galactose and
UDP-N-acetylglucosamine) and different acceptor sugars
(N-acetylglucosamine, galactose, N-acetylgalactosamine). The
beta3GalT genes are distantly related to the Drosophila Brainiac
gene and have the protein coding sequence contained in a single
exon. The beta3GalT proteins also contain conserved sequences not
found in the beta4GalT or alpha3GalT proteins. The carbohydrate
chains synthesized by these enzymes are designated as type 1,
whereas beta4GalT enzymes synthesize type 2 carbohydrate chains.
The ratio of type 1:type 2 chains changes during embryogenesis. By
sequence similarity, the beta3GalT genes fall into at least two
groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3,
beta3GalT5). This gene encodes the most probable candidate for
synthesis of the type 1 Lewis antigens which are frequently found
to be elevated in gastrointestinal and pancreatic cancers. The
encoded protein is inactive with N-linked glycoproteins and
functions in mucin glycosylation. Five transcript variants have
been described which differ in the 5' UTR. All transcript variants
encode an identical protein.
beta-1,3-galactosyltransferase (beta3GalT) gene family. This family
encodes type II membrane-bound glycoproteins with diverse enzymatic
functions using different donor substrates (UDP-galactose and
UDP-N-acetylglucosamine) and different acceptor sugars
(N-acetylglucosamine, galactose, N-acetylgalactosamine). The
beta3GalT genes are distantly related to the Drosophila Brainiac
gene and have the protein coding sequence contained in a single
exon. The beta3GalT proteins also contain conserved sequences not
found in the beta4GalT or alpha3GalT proteins. The carbohydrate
chains synthesized by these enzymes are designated as type 1,
whereas beta4GalT enzymes synthesize type 2 carbohydrate chains.
The ratio of type 1:type 2 chains changes during embryogenesis. By
sequence similarity, the beta3GalT genes fall into at least two
groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3,
beta3GalT5). This gene encodes the most probable candidate for
synthesis of the type 1 Lewis antigens which are frequently found
to be elevated in gastrointestinal and pancreatic cancers. The
encoded protein is inactive with N-linked glycoproteins and
functions in mucin glycosylation. Five transcript variants have
been described which differ in the 5' UTR. All transcript variants
encode an identical protein.
References
Hamshere, M.L., et al. Br J Psychiatry 195(1):23-29(2009)
Lin, C.H., et al. Glycobiology 19(4):418-427(2009)
Seko, A., et al. Tumour Biol. 30(1):43-50(2009)
Mare, L., et al. J. Biol. Chem. 282(1):49-57(2007)
Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :
Lin, C.H., et al. Glycobiology 19(4):418-427(2009)
Seko, A., et al. Tumour Biol. 30(1):43-50(2009)
Mare, L., et al. J. Biol. Chem. 282(1):49-57(2007)
Hu, Y.H., et al. BMC Genomics 7, 155 (2006) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36189
NCBI Official Full Name
beta-1,3-galactosyltransferase 5 isoform a
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5
NCBI Official Symbol
B3GALT5
NCBI Official Synonym Symbols
B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
NCBI Protein Information
beta-1,3-galactosyltransferase 5
UniProt Protein Name
Beta-1,3-galactosyltransferase 5
UniProt Gene Name
B3GALT5
UniProt Synonym Gene Names
Beta-1,3-GalTase 5; Beta3Gal-T5; Beta3GalT5; b3Gal-T5
UniProt Entry Name
B3GT5_HUMAN
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Product Notes
The B3GALT5 b3galt5 (Catalog #AAA288662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 57-84. The B3GALT5 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B3GALT5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the B3GALT5 b3galt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GALT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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