Typical Testing Data/Standard Curve (for reference only)
Rat caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) ELISA Kit | CASP1 elisa kit
Rat Caspase-1, CASP1 ELISA Kit
Gene Names
Casp1; Ice; Il1bc
Reactivity
Rat
Synonyms
caspase 1; apoptosis-related cysteine peptidase (interleukin 1; beta; convertase); Rat Caspase-1; CASP1 ELISA Kit; Rat Caspase-1 (CASP1) ELISA kit; ICE; IL1BC; P45; CASP1 nirs variant 1; IL1B-convertase; apoptosis-related cysteine protease (interleukin 1; convertase) ; caspase-1; interleukin 1-B converting enzyme; interleuk; CASP1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CASP1. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed.
Sequence Length
402
Samples
Serum, plasma, Cell lysates, cell culture supernates
Assay Type
Sandwich
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Apoptosis/Autophagy
Protein Biological Process 3
Apoptosis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CASP1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CASP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,576 Da
NCBI Official Full Name
Caspase-1
NCBI Official Synonym Full Names
caspase 1
NCBI Official Symbol
Casp1
NCBI Official Synonym Symbols
Ice; Il1bc
NCBI Protein Information
caspase-1; p45; CASP-1; IL-1BC; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin 1, beta, convertase; Interleukin 1beta converting enzyme; interleukin-1 beta-converting enzyme
UniProt Protein Name
Caspase-1
UniProt Gene Name
Casp1
UniProt Synonym Gene Names
Il1bc; Il1bce; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
UniProt Entry Name
CASP1_RAT
NCBI Description
apoptotic protease that may play a role in inflammation and apoptosis in male sex organs [RGD, Feb 2006]
Uniprot Description
CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity)
Protein type: Apoptosis; EC 3.4.22.36; Protease
Research Articles on CASP1
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Product Notes
The Rat CASP1 casp1 (Catalog #AAA935341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA935341 ELISA Kit recognizes Rat CASP1. It is sometimes possible for the material contained within the vial of "caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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