Testing Data
Shiga toxin II ELISA Pair Set | stx2B elisa pair set
Shiga toxin II subunit B Matched ELISA Antibody Pair Set, E Coli
Synonyms
Shiga toxin II; Shiga toxin II subunit B Matched ELISA Antibody Pair Set; E Coli; Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B ELISA Pair Set; stx2B elisa pair set
Specificity
Quantitative determination of E Coli Shiga toxin II subunit B
Assay Type
Solid Phase Sandwich ELISA
Detection Range
34.38-2200pg/mL
Sensitivity
34.38pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in dilution buffer before use.
3. Standard Each vial contains 50 ng of recombinant Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B. Reconstitute with 1 mL dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in dilution buffer, and a high standard of 2200 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in dilution buffer before use.
3. Standard Each vial contains 50 ng of recombinant Enterohemorrhagic E Coli (EHEC) STX2B/Shiga toxin II subunit B. Reconstitute with 1 mL dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in dilution buffer, and a high standard of 2200 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for stx2B elisa pair set
E Coli STX2B is a subunit of Stx2. Stx2, together with Stx1, formed a family of related toxins which are known as shiga toxins. Shiga toxins are mainly produced by the bacteria S. dysenteriae and the Shigatoxigenic group of Escherichia coli, which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E Coli (EHEC). A total of 3222 outbreak cases (including 39 deaths) have been reported in northern Germany in May through June 2011. The outbreak strain was typed as an enteroaggregative Shiga-toxin-producing E Coli O104:H4, producing extended-spectrum beta-lactamase. The toxin has two subunits-A and B. E Coli STX2B is the B subunit. It is a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide. Following this, the A subunit is internalised and cleaved into two parts. Stx2 has been found to be approximately 400 times more toxic (as quantified by LD50 in mice) than Stx-1. The Stx1 and Stx2 B subunits form a pentameric structure that binds to globotriaosylceramide receptors on eukaryotic cells and promotes endocytosis.
This Shiga toxin II subunit B Matched ELISA Antibody Pair Set, E Coli is a solid phase sandwich ELISA for quantitative determination of E Coli Shiga toxin II subunit B. It contains E Coli Shiga toxin II subunit B capture antibody, E Coli Shiga toxin II subunit B detector antibody (HRP) and a highly purified E Coli-expressed recombinant E Coli Shiga toxin II subunit B protein.
This Shiga toxin II subunit B Matched ELISA Antibody Pair Set, E Coli is a solid phase sandwich ELISA for quantitative determination of E Coli Shiga toxin II subunit B. It contains E Coli Shiga toxin II subunit B capture antibody, E Coli Shiga toxin II subunit B detector antibody (HRP) and a highly purified E Coli-expressed recombinant E Coli Shiga toxin II subunit B protein.
References
Obata F. et al. (2008) Shiga Toxin 2 Affects the Central Nervous System through Receptor Globotriaosylceramide Localized to Neurons. J Infect Dis. 198 (9): 1398-406.Tironi-Farinati C. et al. (2010) Intracerebroventricular Shiga toxin 2 increases the expression of its receptor globotriaosylceramide and causes dendritic abnormalities. J Neuroimmunol. 222 (1-2): 48-61.Asakura H. et al. (2001) Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals. Epidemiol Infect. 127 (1): 27-36.
Similar Products
Product Notes
The stx2B (Catalog #AAA8118458) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Shiga toxin II, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
