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Western Blot (WB) (Anti-ACER3 Antibody at 1:1000 dilution + human placenta lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human ACER3 Polyclonal Antibody | anti-ACER3 antibody

ACER3 Antibody

Gene Names
ACER3; APHC; PHCA
Reactivity
Human
Applications
Western Blot
Synonyms
ACER3; Polyclonal Antibody; ACER3 Antibody; Purified Rabbit Polyclonal Antibody (Pab); anti-ACER3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Sequence Length
267
Applicable Applications for anti-ACER3 antibody
Western Blot (WB)
Application Notes
WB ~~1:1000
Antigen Source
Human
Antigen Type
A Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-ACER3 Antibody at 1:1000 dilution + human placenta lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-ACER3 Antibody at 1:1000 dilution + human placenta lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-ACER3 antibody
Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.
Product Categories/Family for anti-ACER3 antibody
References
Mao C.,et al.J. Biol. Chem. 276:26577-26588(2001). Li N.,et al.Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Taylor T.D.,et al.Nature 440:497-500(2006). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31552
NCBI Official Full Name
alkaline ceramidase 3 isoform a
NCBI Official Synonym Full Names
alkaline ceramidase 3
NCBI Official Symbol
ACER3
NCBI Official Synonym Symbols
APHC; PHCA
NCBI Protein Information
alkaline ceramidase 3
UniProt Protein Name
Alkaline ceramidase 3
Protein Family
UniProt Gene Name
ACER3
UniProt Synonym Gene Names
APHC; PHCA; AlkCDase 3; Alkaline CDase 3; aPHC
UniProt Entry Name
ACER3_HUMAN

NCBI Description

ACER3: Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. Belongs to the alkaline ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.-; Membrane protein, multi-pass; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to Golgi membrane

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; positive regulation of cell proliferation; sphingosine biosynthetic process; ceramide metabolic process

Uniprot Description

ACER3: Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. Belongs to the alkaline ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to Golgi membrane

Biological Process: ceramide metabolic process; positive regulation of cell proliferation; sphingolipid biosynthetic process; sphingolipid metabolic process; sphingosine biosynthetic process

Research Articles on ACER3

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Product Notes

The ACER3 acer3 (Catalog #AAA9216531) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACER3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACER3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~1:1000. Researchers should empirically determine the suitability of the ACER3 acer3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACER3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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