Testing Data
Factor IX ELISA Pair Set | F9 elisa pair set
Factor IX Matched ELISA Antibody Pair Set, Human
Synonyms
Factor IX; Factor IX Matched ELISA Antibody Pair Set; Human; Human Coagulation Factor IX/FIX/F9 ELISA Pair Set; coagulation factor IX; Coagulation factor 9 Matched ELISA Antibody Pair Set; Coagulation factor IX Matched ELISA Antibody Pair Set; FIX Matched ELISA Antibody Pair Set; HEMB Matched ELISA Antibody Pair Set; P19 Matched ELISA Antibody Pair Set; PTC Matched ELISA Antibody Pair Set; THPH8 Matched ELISA Antibody Pair Set; F9 elisa pair set
Specificity
Quantitative determination of Human Factor IX
Assay Type
Solid Phase Sandwich ELISA
Detection Range
140.63-9000pg/mL
Sensitivity
140.63pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of rabbit anti-Human Coagulation Factor IX/FIX/F9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Coagulation Factor IX/FIX/F9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 240 ng of recombinant Human Coagulation Factor IX/FIX/F9. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 9000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Coagulation Factor IX/FIX/F9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 240 ng of recombinant Human Coagulation Factor IX/FIX/F9. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 9000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for F9 elisa pair set
Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX/F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX/F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX/F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX/F9 are also the cause of recessive X-linked hemophilia B (HEMB) which also known as Christmas disease.
This Factor IX Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Factor IX. It contains Human Factor IX capture antibody, Human Factor IX detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Factor IX protein.
This Factor IX Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Factor IX. It contains Human Factor IX capture antibody, Human Factor IX detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Factor IX protein.
References
Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659. Vidal F., et al., 2000, Br. J. Haematol. 111:549-551. Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675. Espinos C., et al., 2009, Haematologica 88:235-236.
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Product Notes
The F9 (Catalog #AAA8118375) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Factor IX, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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