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603 results for " innate immunity" - showing 350-400


Western Blot (WB) (Western blot analysis of S100A9 expression in human tonsil lysate (MBS179299).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100A9 )

S100A9, Monoclonal Antibody (Cat# AAA179299)

Full Name
Anti-S100A9 Rabbit Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human
Applications
IF, IHC, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS) (Fig-1: Intracellular flow analysis of PE conjugated mTLR9 antibody in mouse splenocytes using 0.5 ug/10^6 cells of PE conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with PE.)

mTLR9, Monoclonal Antibody (Cat# AAA668971)

Full Name
Monoclonal Antibody to mTLR9 (Clone: ABM4D70) PE conjugated
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified
Protein G Chromatography
Pricing
$350/100 Tests | $170/25 Tests | $1,510/5x100 Tests
Western Blot (WB) (Western blot analysis of Phospho-BTK (Y223) expression in Raji cell lysate treated with pervanadate (MBS1750226).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTK monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BTK )

BTK, Monoclonal Antibody (Cat# AAA1750226)

Full Name
Anti-Phospho-BTK (Y223) Rabbit Monoclonal Antibody
Gene Names
BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of p38 MAPK expression in Jurkat cell lysate (MBS179164).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK14 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK14 )

p38 MAPK, Monoclonal Antibody (Cat# AAA179164)

Full Name
Anti-p38 MAPK Rabbit Monoclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Anti-IL1RAP Antibody (Center) at 1:2000 dilution + Mouse liver lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

IL1RAP, Polyclonal Antibody (Cat# AAA9232320)

Full Name
IL1RAP Antibody (Center)
Gene Names
IL1RAP; IL1R3; C3orf13; IL-1RAcP
Reactivity
Human, Mouse
Applications
WB, FC/FACS, IHC

MAVS, Blocking Peptide (Cat# AAA5400632)

Full Name
MAVS Blocking Peptide
Gene Names
MAVS; IPS1; VISA; IPS-1; CARDIF
Applications
Immunodepletion, Immunocompetition
Pricing
$280/0.25 mg | $1,110/5x0.25 mg

MEFV, Polyclonal Antibody (Cat# AAA7109900)

Full Name
MEFV Antibody, HRP conjugated
Gene Names
MEFV; FMF; MEF; TRIM20
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

MEFV, Polyclonal Antibody (Cat# AAA7109901)

Full Name
MEFV Antibody, FITC conjugated
Gene Names
MEFV; FMF; MEF; TRIM20
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of Phospho-IRF3 (S386) expression in MCF7 cell lysate (MBS1750216).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF3 )

IRF3, Monoclonal Antibody (Cat# AAA1750216)

Full Name
Anti-Phospho-IRF3 (S386) Rabbit Monoclonal Antibody
Gene Names
IRF3; IIAE7
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of TLR2 using anti- TLR2 antibody (MBS1750331).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TLR2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TLR2 at approximately 89KD. The expected band size for TLR2 is at 89KD. )

TLR2, Polyclonal Antibody (Cat# AAA1750331)

Full Name
Anti-TLR2 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg

MEFV, Polyclonal Antibody (Cat# AAA7109902)

Full Name
MEFV Antibody, Biotin conjugated
Gene Names
MEFV; FMF; MEF; TRIM20
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Flow Cytometry (FC/FACS) (Fig-1: Cell surface flow analysis of FITC conjugated mTLR4 antibody (cloneMTS510) in mSplenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents FITC conjugated anti-mTLR4 antibody.)

mTLR4 CD284, Monoclonal Antibody (Cat# AAA668965)

Full Name
Monoclonal Antibody to mTLR4 CD284 (Clone: MTS510) FITC Conjugated
Gene Names
Tlr4; Lps; Ly87; Ran/M1; Rasl2-8
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified
Protein G Purification
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Western Blot (WB) (Western blot analysis of MEK5 expression in Hela cell lysate (MBS179884).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K7 )

MEK7, Monoclonal Antibody (Cat# AAA179884)

Full Name
Anti-MEK7 Rabbit Monoclonal Antibody
Gene Names
MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of JNK3 expression in HeLa lysate (MBS1750010).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK10 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK10 )

JNK3, Monoclonal Antibody (Cat# AAA1750010)

Full Name
Anti-JNK3 Rabbit Monoclonal Antibody
Gene Names
MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of PI 3 Kinase Class 3 expression in 293T cell lysate (MBS179834).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3C3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3C3 )

PI 3 Kinase Class 3, Monoclonal Antibody (Cat# AAA179834)

Full Name
Anti-PI 3 Kinase Class 3 Rabbit Monoclonal Antibody
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS) (Fig-2: Intracellular flow analysis of TLR9 FITC conjugated in hPBMC (Lymphocytes) using 0.5 ug/10^6 cells of TLR9 FITC conjugated antibody (Clone: ABM1C51). Green represents isotype control; red represents anti-TLR9 FITC conjugated antibody.)

TLR9, Monoclonal Antibody (Cat# AAA668949)

Full Name
Monoclonal Antibody to TLR9 (Clone: ABM1C51) FITC conjugated
Gene Names
TLR9; CD289
Reactivity
Mouse, Human
Applications
FC/FACS
Purity
Purified
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Western Blot (WB) (Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (SPC-741) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.)

PIK3CA, Polyclonal Antibody (Cat# AAA8000769)

Full Name
PIK3CA Antibody
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000258)

Full Name
CCR2 Antibody: Biotin
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB) (Western blot analysis of SGK1 expression in A431 cell lysate (MBS179857).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRAK3 )

IRAK3/Irak M, Monoclonal Antibody (Cat# AAA179857)

Full Name
Anti-IRAK3/Irak M Rabbit Monoclonal Antibody
Gene Names
IRAK3; ASRT5; IRAKM
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Adenosine receptor A1 Antibody (D) Composite.)

Adenosine receptor A1, Polyclonal Antibody (Cat# AAA8000244)

Full Name
Adenosine receptor A1 Antibody: PerCP
Gene Names
ADORA1; RDC7
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (MBS1750573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD. )

CD40L, Polyclonal Antibody (Cat# AAA1750573)

Full Name
Anti-CD40L Picoband antibody
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of MyD88 using anti-MyD88 antibody (MBS1750308).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse spleen tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MyD88 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MyD88 at approximately 33KD. The expected band size for MyD88 is at 33KD. )

MyD88, Polyclonal Antibody (Cat# AAA1750308)

Full Name
Anti-MyD88 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody(MBS179775)DES was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DES Antibody (MBS179775)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Desmin, Monoclonal Antibody (Cat# AAA179775)

Full Name
Anti-Desmin Rabbit Monoclonal Antibody
Gene Names
DES; CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Hexokinase 1 Antibody(MBS179676)HK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HK1 Antibody (MBS179676)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Hexokinase 1, Monoclonal Antibody (Cat# AAA179676)

Full Name
Anti-Hexokinase 1 Rabbit Monoclonal Antibody
Gene Names
HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of MyD88 using anti-MyD88 antibody (MBS1750309).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MyD88 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MyD88 at approximately 33KD. The expected band size for MyD88 is at 33KD. )

MyD88, Polyclonal Antibody (Cat# AAA1750309)

Full Name
Anti-MyD88 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of CD59 expression in HUVEC cell lysate (MBS179488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD59 )

CD59, Monoclonal Antibody (Cat# AAA179488)

Full Name
Anti-CD59 Rabbit Monoclonal Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p38 Antibody(MBS179165)MAPK14 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK14 Antibody (MBS179165)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

p38, Monoclonal Antibody (Cat# AAA179165)

Full Name
Anti-p38 Rabbit Monoclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
ChIP, FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)

IL-23R, ELISA Kit (Cat# AAA1751135)

Full Name
Mouse IL-23R PicoKine ELISA Kit
Gene Names
Il23r; IL-23R
Reactivity
Mouse
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46368(C1orf106 Antibody) at dilution 1/80, on the right is treated with fusion protein. (Original magnification: x200))

C1orf106, Polyclonal Antibody (Cat# AAA9429516)

Full Name
C1orf106 Antibody
Gene Names
C1orf106; INAVA
Reactivity
Human
Applications
IHC
Purity
Antigen affinity purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC) (IHC image of MBS7109899 diluted at 1:200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

MEFV, Polyclonal Antibody (Cat# AAA7109899)

Full Name
MEFV Antibody
Gene Names
MEFV; FMF; MEF; TRIM20
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of IL1RA expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (MBS179404).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RN )

IL1RA, Monoclonal Antibody (Cat# AAA179404)

Full Name
Anti-IL1RA Rabbit Monoclonal Antibody
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)

RAC1, Polyclonal Antibody (Cat# AAA8001068)

Full Name
RAC1 Antibody: Biotin
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Figure 1. Western blot analysis of CCR1 using anti-CCR1 antibody (MBS1750726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR1 at approximately 48KD. The expected band size for CCR1 is at 41KD. )

CCR1, Polyclonal Antibody (Cat# AAA1750726)

Full Name
Anti-CCR1 Picoband Antibody
Gene Names
Ccr1; Cmkbr1; Mip-1a-R
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rac1/2/3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (MBS179055).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAC1 )

Rac1/2/3, Monoclonal Antibody (Cat# AAA179055)

Full Name
Anti-Rac1/2/3 Rabbit Monoclonal Antibody
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of ATF2 expression in HeLa cell lysate (MBS179489).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATF2 )

ATF2, Monoclonal Antibody (Cat# AAA179489)

Full Name
Anti-ATF2 Rabbit Monoclonal Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS) (Fig-1: Intracellular flow analysis of FITC-conjugated mTLR9 antibody in mouse splenocytes using 0.25 ug/10^6 cells of FITC-conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with FITC.)

mTLR9, Monoclonal Antibody (Cat# AAA668969)

Full Name
Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified
Protein G Chromatography
Pricing
$165/0.025 mg | $325/100 Tests | $1,405/5x100 Tests
Flow Cytometry (FC/FACS) (Fig-1: Intracellular flow analysis of TLR3 FITC conjugated in THP-1 cell line using 0.5 ug/10^6 cells of TLR3 FITC conjugated antibody (Clone: ABM15D5). Green represents isotype control; red represents anti-TLR3 FITC conjugated antibody.)

TLR3, Monoclonal Antibody (Cat# AAA668952)

Full Name
Monoclonal Antibody to TLR3 (Clone: ABM15D5)-FITC conjugated
Gene Names
TLR3; CD283; IIAE2
Reactivity
Human
Applications
FC/FACS
Purity
Purified
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg

MASP1 / Mannan-Binding Lectin Serine Protease 1, Recombinant Protein (Cat# AAA2903052)

Full Name
Human MASP1 / Mannan-Binding Lectin Serine Protease 1 Recombinant Protein
Gene Names
MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000248)

Full Name
CCR2 Antibody: ATTO 390
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000262)

Full Name
CCR2 Antibody: PerCP
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000249)

Full Name
CCR2 Antibody: ATTO 488
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB) (Western blot analysis of IRF5 expression in Ramos cell lysate (MBS179504).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF5 )

IRF5, Monoclonal Antibody (Cat# AAA179504)

Full Name
Anti-IRF5 Rabbit Monoclonal Antibody
Gene Names
IRF5; SLEB10
Reactivity
Human
Applications
FC/FACS, IHC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of RALBP1 expression in HeLa cell lysate (MBS179648).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RALBP1 )

RALBP1, Monoclonal Antibody (Cat# AAA179648)

Full Name
Anti-RALBP1 Rabbit Monoclonal Antibody
Gene Names
RALBP1; RIP1; RLIP1; RLIP76
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (MBS1750456). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA at approximately 28KD. The expected band size for IL1RA is at 20KD. )

IL1RA, Polyclonal Antibody (Cat# AAA1750456)

Full Name
Anti-IL1RA Picoband Antibody
Gene Names
Il1rn; IL-1ra
Reactivity
Rat
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB) (Western blot analysis of BPI expression in THP1 lysate (MBS179658).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BPI monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BPI )

BPI, Monoclonal Antibody (Cat# AAA179658)

Full Name
Anti-BPI Rabbit Monoclonal Antibody
Gene Names
BPI; rBPI; BPIFD1
Reactivity
Human
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of TLR9 expression in Raji cell lysate (MBS179181).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TLR9 )

TLR9, Monoclonal Antibody (Cat# AAA179181)

Full Name
Anti-TLR9 Rabbit Monoclonal Antibody
Gene Names
TLR9; CD289
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (MBS1750473).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD. )

MIP-3 Alpha/CCL20, Polyclonal Antibody (Cat# AAA1750473)

Full Name
Anti-MIP-3 Alpha/CCL20 Picoband Antibody
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Flow Cytometry (FC/FACS) (Fig-1: Intracellular flow analysis of hTLR5 FITC conjugated in hTLR5 transfected cell line using 0.5 ug/10^6 cells of hTLR5 FITC conjugated antibody (Clone: ABM22G1). Green represents isotype control; red represents anti-hTLR5 FITC conjugated antibody.)

TLR5, Monoclonal Antibody (Cat# AAA668959)

Full Name
Monoclonal Antibody to TLR5 (Clone: ABM22G1) FITC conjugated
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human
Applications
FC/FACS
Purity
Purified
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000256)

Full Name
CCR2 Antibody: Alkaline Phosphatase
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CCR2 Polyclonal Antibody (SPC-704) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~41.9 kDa.)

CCR2, Polyclonal Antibody (Cat# AAA8000259)

Full Name
CCR2 Antibody: FITC
Gene Names
CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
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