Rabbit anti-Human XAF1 Polyclonal Antibody | anti-XAF1 antibody

Rabbit Polyclonal to Human XAF1

Cat. Num. AAA241350

• Gene Names: XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: XAF1; Polyclonal Antibody; Rabbit Polyclonal to Human XAF1; Anti-XAF1 Antibody IHC-plus; BIRC4 binding protein; BIRC4-binding protein; BIRC4BP; HSXIAPAF1; XIAP-associated factor 1; XIAPAF1; XIAP associated factor 1; Human XAF1; anti-XAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 301

• Applicable Applications for anti-XAF1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human XAF1.
• Immunogen Type: Synthetic peptide
• Immunogen: XAF1 antibody was raised against synthetic peptide from human XAF1.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-XAF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of XAF-1 in human spleen lysate with XAF-1 antibody (IN) at 1 (lane A), 2 (lane B), and 4 (lane C) ug/ml, respectively.)

Related Product Information for anti-XAF1 antibody

XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines.

Product Categories/Family for anti-XAF1 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 40288191
• NCBI GeneID: 54739
• NCBI Accession #: NP_059993.2
• NCBI GenBank Nucleotide #: NM_017523.3
• UniProt Accession #: Q6GPH4; Q6MZE8; Q8N557; Q99982; A2T931; A2T932; A8K2L1; A8K9Y3; D3DTM6
• Molecular Weight: 34,543 Da
• NCBI Official Full Name: XIAP-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: XAF1
• NCBI Official Synonym Symbols: BIRC4BP; XIAPAF1; HSXIAPAF1
• NCBI Protein Information: XIAP-associated factor 1; BIRC4 binding protein; BIRC4-binding protein
• UniProt Protein Name: XIAP-associated factor 1
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XAF1
• UniProt Synonym Gene Names: BIRC4BP; XIAPAF1
• UniProt Entry Name: XAF1_HUMAN

NCBI Description

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: mitochondrion; cytosol; nucleus

Molecular Function: zinc ion binding

Biological Process: apoptosis; cytokine and chemokine mediated signaling pathway; negative regulation of protein complex assembly