Flow Cytometry (FC/FACS)
(Fig-1: Intracellular flow analysis of FITC-conjugated mTLR9 antibody in mouse splenocytes using 0.25 ug/10^6 cells of FITC-conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with FITC.)
Rat anti-Mouse mTLR9 Monoclonal Antibody | anti-mTLR9 antibody
Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
PurifiedProtein G Chromatography
Synonyms
mTLR9; Monoclonal Antibody; Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated; Tlr9; CD289; anti-mTLR9 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
ABM4D70
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
0.2 mg/ml in Tris buffer containing 0.05% Azide
Sequence Length
1032
Applicable Applications for anti-mTLR9 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 0.25-1 ug/10^6 cells
Antigen
mTLR9
Immunogen Information
A partial length recombinant mTLR9 protein (amino acids 602-860) was used as the immunogen for this antibody.
Conjugation
FITC
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
Flow Cytometry (FC/FACS)
(Fig-1: Intracellular flow analysis of FITC-conjugated mTLR9 antibody in mouse splenocytes using 0.25 ug/10^6 cells of FITC-conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with FITC.)
Flow Cytometry (FC/FACS)
(Fig-1: Intracellular flow analysis of FITC-conjugated mTLR9 antibody in mouse splenocytes using 0.25 ug/10^6 cells of FITC-conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with FITC.)
Related Product Information for anti-mTLR9 antibody
Mouse Toll-like receptor 9 (mTLR9) is an innate immune sensor for microbial DNA that erroneously responds to self DNA in autoimmune disease. TLR9 is normally expressed in endosomes/lysosomes where it is activated by pathogen-derived DNA. It has modulatory effect on the mucosal immune system, influence susceptibility to allergic sensitization to foods (22718261). TLR9 recognizes unmethylated CpG-containing DNA commonly found in bacteria. TLR9 signaling plays an important role in the development of robust protective immunity, proper recruitment and function of effector cells (lymphocytes and macrophages), and, ultimately, effective cryptococcal clearance from the infected lungs (20581055). It is involved in the pathogenesis of cardiovascular diseases, such as hypertension and heart failure.
Product Categories/Family for anti-mTLR9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116,412 Da
NCBI Official Full Name
toll-like receptor 9
NCBI Official Synonym Full Names
toll-like receptor 9
NCBI Official Symbol
Tlr9
NCBI Protein Information
toll-like receptor 9
UniProt Protein Name
Toll-like receptor 9
UniProt Gene Name
Tlr9
Uniprot Description
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production ().
Research Articles on mTLR9
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Product Notes
The mTLR9 tlr9 (Catalog #AAA668969) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's mTLR9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 0.25-1 ug/10^6 cells. Researchers should empirically determine the suitability of the mTLR9 tlr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTLR9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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