Rat anti-Mouse mTLR9 Monoclonal Antibody | anti-mTLR9 antibody

Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated

Cat. Num. AAA668969

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified; Protein G Chromatography
• Synonyms: mTLR9; Monoclonal Antibody; Monoclonal Antibody to mTLR9 (Clone: ABM4D70) FITC Conjugated; Tlr9; CD289; anti-mTLR9 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone Number: ABM4D70
• Purity/Purification: Purified; Protein G Chromatography
• Form/Format: 0.2 mg/ml in Tris buffer containing 0.05% Azide
• Sequence Length: 1032

• Applicable Applications for anti-mTLR9 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 0.25-1 ug/10^6 cells
• Antigen: mTLR9
• Immunogen Information: A partial length recombinant mTLR9 protein (amino acids 602-860) was used as the immunogen for this antibody.
• Conjugation: FITC
• Preparation and Storage: Store the antibody at 4 degree C; stable for 6 months.

Flow Cytometry (FC/FACS)

(Fig-1: Intracellular flow analysis of FITC-conjugated mTLR9 antibody in mouse splenocytes using 0.25 ug/10^6 cells of FITC-conjugated mTLR9 antibody (Clone: ABM4D70). Green represents isotype control; red represents anti-mTLR9 antibody conjugated with FITC.)

Related Product Information for anti-mTLR9 antibody

Mouse Toll-like receptor 9 (mTLR9) is an innate immune sensor for microbial DNA that erroneously responds to self DNA in autoimmune disease. TLR9 is normally expressed in endosomes/lysosomes where it is activated by pathogen-derived DNA. It has modulatory effect on the mucosal immune system, influence susceptibility to allergic sensitization to foods (22718261). TLR9 recognizes unmethylated CpG-containing DNA commonly found in bacteria. TLR9 signaling plays an important role in the development of robust protective immunity, proper recruitment and function of effector cells (lymphocytes and macrophages), and, ultimately, effective cryptococcal clearance from the infected lungs (20581055). It is involved in the pathogenesis of cardiovascular diseases, such as hypertension and heart failure.

Product Categories/Family for anti-mTLR9 antibody

TLR and Innate Immunity

NCBI and Uniprot Product Information

• NCBI GI #: 157057166
• NCBI GeneID: 81897
• NCBI Accession #: NP_112455.2
• NCBI GenBank Nucleotide #: NM_031178.2
• UniProt Accession #: Q9EQU3; Q4L0K3; Q4L0K4; Q99MF2; Q99MQ8; F8VPN5
• Molecular Weight: 116,412 Da
• NCBI Official Full Name: toll-like receptor 9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: Tlr9
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• UniProt Gene Name: Tlr9

Uniprot Description

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:18931679, PubMed:21402738, PubMed:14993594, PubMed:17474149, PubMed:25686612, PubMed:18820679). Plays a role in defense against systemic mouse cytomegalovirus infection (PubMed:14993594). Controls lymphocyte response to Helicobacter infection (PubMed:17474149). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production ().