Rabbit TLR2 Polyclonal Antibody | anti-TLR2 antibody

Anti-TLR2 Picoband Antibody

Cat. Num. AAA1750331

• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: TLR2; Polyclonal Antibody; Anti-TLR2 Picoband Antibody; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; CD282; TIL4; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Purity/Purification: Immunogen affinity purified
• Form/Format: Lyophilized
• Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (varies by lot)
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: Western Blot (WB)
• Application Notes: WB: 0.1-0.5mug/ml
• Immunogen: E Coli-derived human TLR2 recombinant protein (Position: H610-S784). Human TLR2 shares 87.4% amino acid (aa) sequence identity with mouse TLR2.
• Subcellular Localization: Membrane; Single-pass type I membrane protein.
• Tissue Specificity: Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Figure 1. Western blot analysis of TLR2 using anti- TLR2 antibody (MBS1750331).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TLR2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TLR2 at approximately 89KD. The expected band size for TLR2 is at 89KD. )

Related Product Information for anti-TLR2 antibody

Description: Toll-like receptor 2, also known as TLR-2, is a protein that in humans is encoded by the TLR2 gene. It has also been designated as CD282 (cluster of differentiation 282). TLR-2 plays a role in the immune system. And TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
Protein Function: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

Product Categories/Family for anti-TLR2 antibody

Immunology; Innate Immunity; Macrophage/Inflamm.; Immunology; immunoGlobulins; Receptors; Microbiology; Protein; Human Protein; Defensin; Signal Transduction; Adapters; transmembrane; Cardiovascular; Atherosclerosis; Vascular Inflammation; Inflammatory Me

NCBI and Uniprot Product Information

• NCBI GI #: 974005295
• NCBI GeneID: 7097
• NCBI Accession #: NP_001305716.1
• NCBI GenBank Nucleotide #: NM_001318787.1
• UniProt Accession #: O60603; O15454; Q8NI00; B3Y612; D1CS45; D1CS48; D1CS49
• Molecular Weight: 89838 MW
• NCBI Official Full Name: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4

Uniprot Description

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852, PubMed:17889651). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM ().