Search results for " biotin conjugated "

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Testing Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))

HISTIDINE TAG, Monoclonal Antibody (Cat# AAA211163)

Full Name

MOUSE ANTI HISTIDINE TAG:HRP

Applications

EIA, WB

Pricing

$490/0.1 mg | $2,060/5x0.1 mg

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CD204, Monoclonal Antibody (Cat# AAA210654)

Full Name

RAT ANTI MOUSE CD204:FITC

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD19 (MBS212401))

CD19, Monoclonal Antibody (Cat# AAA210021)

Full Name

MOUSE ANTI HUMAN CD19:APC

Gene Names

CD19; B4; CVID3

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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Testing Data (Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin (MBS211202))

CD71, Monoclonal Antibody (Cat# AAA212212)

Full Name

MOUSE ANTI RAT CD71

Gene Names

Tfrc; Trfr

Applications

FC/FACS, IF

Pricing

$345/0.1 mg | $1,405/5x0.1 mg

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Western Blot (WB) (Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (MBS178867).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysate,Lane 2: human 22RV1 cell lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat thymus tissue lysate,Lane 5: mouse spleen tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD44 at approximately 88KD. The expected band size for CD44 is at 82KD. )

CD44, Polyclonal Antibody (Cat# AAA178867)

Full Name

Anti-CD44 Picoband antibody

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD29 (MBS212408))

CD29, Monoclonal Antibody (Cat# AAA210807)

Full Name

MOUSE ANTI HUMAN CD29:FITC

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Western Blot (WB) (All lanes: Transcription intermediary factor 1-beta antibody at at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 92 kDa Observed band size: 92 kDa Immunohistochemistry)

Transcription intermediary factor 1-beta, Polyclonal Antibody (Cat# AAA715940)

Full Name

Rabbit anti-human Transcription intermediary factor 1-beta polyclonal Antibody, Biotin conjugated

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human

Applications

EIA

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

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Testing Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)

CD172a, Monoclonal Antibody (Cat# AAA210478)

Full Name

MOUSE ANTI RAT CD172a:Biotin

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

FC/FACS

Pricing

$425/0.1 mg | $1,755/5x0.1 mg

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Immunohistochemistry (IHC) (Figure 1. IHC analysis of SMC3 using anti-SMC3 antibody (M01930-1).SMC3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-SMC3 Antibody (M01930-1) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

SMC3, Monoclonal Antibody (Cat# AAA1752151)

Full Name

Anti-SMC3 Antibody (monoclonal, 4C12)

Gene Names

SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Immunogen Affinity Purified

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

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Western Blot (WB) (Figure 1. Western blot analysis of Calpastatin using anti-Calpastatin antibody (MBS178604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: COLO320 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpastatin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calpastatin at approximately 100KD. The expected band size for Calpastatin is at 76KD. )

Calpastatin, Polyclonal Antibody (Cat# AAA178604)

Full Name

Anti-Calpastatin Antibody

Gene Names

CAST; BS-17; PLACK

Reactivity

Human

Applications

WB, IHC

Purity

Immunogen affinity purified.

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

CD119, Monoclonal Antibody (Cat# AAA2553980)

Full Name

Anti-Mouse CD119 Monoclonal Antibody (Biotin Conjugated)

Gene Names

Ifngr1; Ifgr; CD119; Ifngr; Nktar; IFN-gammaR

Reactivity

Mouse

Applications

FC/FACS

Pricing

$140/0.05 mg | $170/0.1 mg | $755/5x0.1 mg

CD22, Monoclonal Antibody (Cat# AAA2553784)

Full Name

Anti-Mouse CD22 Monoclonal Antibody (Biotin Conjugated)

Gene Names

Cd22; Lyb8; Lyb-8; A530093D23

Reactivity

Mouse

Applications

FC/FACS

Pricing

$205/0.1 mg | $115/0.025 mg | $925/5x0.1 mg

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Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD312 (MBS215927))

CD312, Monoclonal Antibody (Cat# AAA215935)

Full Name

MOUSE ANTI HUMAN CD312:RPE

Gene Names

EMR2; CD312

Applications

FC/FACS

Pricing

$550/100 Tests | $2,320/5x100 Tests

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F4/80, Monoclonal Antibody (Cat# AAA216410)

Full Name

RAT ANTI MOUSE F4/80:Biotin

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

FC/FACS

Pricing

$670/0.5 mg | $2,870/5x0.5 mg

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CD55, Monoclonal Antibody (Cat# AAA225631)

Full Name

Mouse Anti Human CD55: FITC

Gene Names

CD55; CR; TC; DAF; CROM; CHAPLE

Reactivity

Human

Applications

FC/FACS

Purity

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Testing Data (Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE (MBS216020))

Ly-6C, Monoclonal Antibody (Cat# AAA216017)

Full Name

RAT ANTI MOUSE Ly-6C:Biotin

Gene Names

Ly6c2; Ly-6C2; Ly-6C.2

Applications

FC/FACS

Pricing

$460/0.1 mg | $1,910/5x0.1 mg

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Testing Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))

HISTIDINE TAG, Monoclonal Antibody (Cat# AAA219367)

Full Name

MOUSE ANTI HISTIDINE TAG:ALK.PHOS.

Applications

EIA, WB

Pricing

$445/0.1 mg | $1,845/5x0.1 mg

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Testing Data #1 (Staining of human peripheral blood lymphocytes with Mouse anti Human CD29 (MBS212408))

CD29, Monoclonal Antibody (Cat# AAA235343)

Full Name

MOUSE ANTI HUMAN CD29:RPE

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD14-IgG:Endotoxin Low (MBS224504))

CD14, Monoclonal Antibody (Cat# AAA213858)

Full Name

MOUSE ANTI HUMAN CD14:RPE

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216584)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin

Applications

FC/FACS

Pricing

$210/0.025 mg | $800/5x0.025 mg

CD235, Monoclonal Antibody (Cat# AAA2553872)

Full Name

Anti-Human CD235 Monoclonal Antibody (Biotin Conjugated)

Gene Names

GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)

Reactivity

Human

Applications

FC/FACS

Pricing

$190/0.1 mg | $105/0.025 mg | $840/5x0.1 mg

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Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD54:FITC (MBS210744))

CD54, Monoclonal Antibody (Cat# AAA215119)

Full Name

MOUSE ANTI HUMAN CD54:FITC

Gene Names

ICAM1; BB2; CD54; P3.58

Applications

FC/FACS

Pricing

$195/0.025 mg | $730/5x0.025 mg

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Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dibromo-tyrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Dibromo-tyrosine, Monoclonal Antibody (Cat# AAA808621)

Full Name

Dibromo-tyrosine Antibody, Clone 6G3: Biotin

Reactivity

Species Independent

Applications

WB, ICC, IF, EIA

Purity

Protein G Purified

Pricing

$555/0.1 mg | $2,255/5x0.1 mg

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Testing Data (Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:RPE (MBS213782))

CD61, Monoclonal Antibody (Cat# AAA210935)

Full Name

HAMSTER ANTI MOUSE CD61:FITC

Gene Names

Itgb3; CD61; GP3A; INGRB3

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Testing Data (Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:RPE)

CD61, Monoclonal Antibody (Cat# AAA213782)

Full Name

HAMSTER ANTI MOUSE CD61:RPE

Gene Names

Itgb3; CD61; GP3A; INGRB3

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA213859)

Full Name

RAT ANTI MOUSE CD8 ALPHA:RPE

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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CD204, Monoclonal Antibody (Cat# AAA210655)

Full Name

RAT ANTI MOUSE CD204:FITC

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

FC/FACS

Pricing

$260/0.05 mg | $1,020/5x0.05 mg

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Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Malondialdehyde, Monoclonal Antibody (Cat# AAA808765)

Full Name

Malondialdehyde Antibody, Clone 6H6: Biotin

Reactivity

Species Independent

Applications

WB, ICC, IF, FC/FACS, EIA

Purity

Protein G Purified

Pricing

$555/0.1 mg | $2,255/5x0.1 mg

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CD14, Monoclonal Antibody (Cat# AAA213636)

Full Name

MOUSE ANTI HUMAN CD14:RPE

Applications

FC/FACS

Pricing

$500/100 Tests | $2,090/5x100 Tests

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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Alexa Fluor 647 (MBS211993A647))

CD38, Monoclonal Antibody (Cat# AAA210303)

Full Name

MOUSE ANTI HUMAN CD38:Biotin

Gene Names

CD38; T10; ADPRC 1

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488 )

MHC CLASS II RT1B, Monoclonal Antibody (Cat# AAA210489)

Full Name

MOUSE ANTI RAT MHC CLASS II RT1B:Biotin

Applications

FC/FACS

Pricing

$425/0.1 mg | $1,755/5x0.1 mg

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Western Blot (WB) (Figure 1. Western blot analysis of TLR1 using anti-TLR1 antibody (MBS1750402).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: mouse small intestine tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD. )

TLR1, Polyclonal Antibody (Cat# AAA1750402)

Full Name

Anti-TLR1 Picoband antibody

Gene Names

TLR1; TIL; CD281; rsc786; TIL. LPRS5

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, FC/FACS, IHC, ICC, WB

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

CD117, Monoclonal Antibody (Cat# AAA2553896)

Full Name

Anti-Mouse CD117 Monoclonal Antibody (Biotin Conjugated)

Reactivity

Mouse

Applications

FC/FACS

Pricing

$130/0.05 mg | $155/0.1 mg | $700/5x0.1 mg

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Testing Data (Staining of human peripheral blood platelets with Mouse anti Human CD9:RPE (MBS213834))

CD9, Monoclonal Antibody (Cat# AAA216385)

Full Name

MOUSE ANTI HUMAN CD9:Biotin

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Applications

FC/FACS

Pricing

$195/0.025 mg | $730/5x0.025 mg

CD18, Monoclonal Antibody (Cat# AAA2553976)

Full Name

Anti-Mouse CD18 Monoclonal Antibody (Biotin Conjugated)

Gene Names

Itgb2; 2E6; LAD; Cd18; Lfa1; MF17; LCAMB; AI528527

Reactivity

Mouse

Applications

FC/FACS

Pricing

$165/0.05 mg | $215/0.1 mg | $960/5x0.1 mg

CD18, Monoclonal Antibody (Cat# AAA2553840)

Full Name

Anti-Human CD18 Monoclonal Antibody (Biotin Conjugated)

Reactivity

Human

Applications

FC/FACS

Pricing

$170/0.1 mg | $100/0.025 mg | $755/5x0.1 mg

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CD14, Monoclonal Antibody (Cat# AAA219364)

Full Name

MOUSE ANTI HUMAN CD14:Biotin

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Immunohistochemistry (IHC) (Immunohistochemistry)

Vitamin D-binding protein, Polyclonal Antibody (Cat# AAA1491956)

Full Name

Rabbit anti-human Vitamin D-binding protein polyclonal Antibody, Biotin conjugated

Reactivity

Human

Applications

EIA

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg

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CD204, Monoclonal Antibody (Cat# AAA214935)

Full Name

RAT ANTI MOUSE CD204:Biotin

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

FC/FACS

Pricing

$195/0.025 mg | $730/5x0.025 mg

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Testing Data (Staining of human peripheral blood platelets with Mouse anti Human CD31:FITC)

CD31, Monoclonal Antibody (Cat# AAA210760)

Full Name

MOUSE ANTI HUMAN CD31:FITC

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Applications

FC/FACS

Pricing

$415/0.1 mg | $1,710/5x0.1 mg

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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD7:Biotin (MBS210314))

CD7, Monoclonal Antibody (Cat# AAA210630)

Full Name

MOUSE ANTI HUMAN CD7:FITC

Gene Names

CD7; GP40; TP41; Tp40; LEU-9

Applications

FC/FACS

Pricing

$430/100 Tests | $1,775/5x100 Tests

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Western Blot (WB) (Figure 1. Western blot analysis of Bik using anti-Bik antibody (MBS178226). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate, Lane 2: Rat Gaster Tissue Lysate, Lane 3: Rat Intestine Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: A549 Whole Cell Lysate, Lane 6: SKOV Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bik antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bik at approximately 22KD. The expected band size for Bik is at 22KD. )

Bik, Polyclonal Antibody (Cat# AAA178226)

Full Name

Anti-Bik Antibody

Gene Names

BIK; BP4; NBK; BIP1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Immunogen Affinity Purified

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

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Immunohistochemistry (IHC) (Figure 1. IHC analysis of RbAp48 using anti-RbAp48 antibody (M02702-1).RbAp48 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-RbAp48 Antibody (M02702-1) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

RbAp48, Monoclonal Antibody (Cat# AAA1752230)

Full Name

Anti-RbAp48 Antibody (monoclonal, 9F3)

Gene Names

RBBP4; NURF55; RBAP48; lin-53

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Immunogen Affinity Purified

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

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Western Blot (WB) (Figure 1. Western blot analysis of Dynamin 1 using anti-Dynamin 1 antibody (MBS1750776). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dynamin 1 at approximately 97KD. The expected band size for Dynamin 1 is at 97KD. )