Mouse CD312 Monoclonal Antibody | anti-CD312 antibody

MOUSE ANTI HUMAN CD312:RPE

Cat. Num. AAA215935

• Gene Names: EMR2; CD312
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD312; Monoclonal Antibody; MOUSE ANTI HUMAN CD312:RPE; anti-CD312 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2A1
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 765

• Applicable Applications for anti-CD312 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1.0 ml distilled water; Perservative Stabilisers
• Immunogen: NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312 (MBS215927))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD312: FITC (MBS215933))

Testing Data

(Staining of human peripheral blood monocytes probed with Mouse anti Human CD312 : Alexa Fluor 647 (MBS215927A647))

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD312: Biotin (MBS215930))

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312: RPE)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488 (MBS215927A488))

Related Product Information for anti-CD312 antibody

Mouse anti Human CD312 antibody, clone 2A1 recognizes human EMR2, a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2. Clone 2A1 specifically recognizes the stalk region of EMR2.

NCBI and Uniprot Product Information

• NCBI GI #: 402743849
• NCBI GeneID: 30817
• NCBI Accession #: NP_001257981.1
• NCBI GenBank Nucleotide #: NM_001271052.1
• UniProt Accession #: Q9UHX3; Q8NG96; Q9Y4B1; B4DQ96; E7ESD7; E9PBR1; E9PEL6; E9PFQ5; E9PG91
• Molecular Weight: 84,079 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 2 isoform h
• NCBI Official Synonym Full Names: egf-like module containing, mucin-like, hormone receptor-like 2
• NCBI Official Symbol: EMR2
• NCBI Official Synonym Symbols: CD312
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Gene Name: EMR2
• UniProt Entry Name: EMR2_HUMAN

NCBI Description

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

EMR2: Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell migration; cell adhesion; inflammatory response