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Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
Mouse HISTIDINE TAG Monoclonal Antibody
MOUSE ANTI HISTIDINE TAG:ALK.PHOS.
Applications
ELISA, Western Blot
Synonyms
HISTIDINE TAG; Monoclonal Antibody; MOUSE ANTI HISTIDINE TAG:ALK.PHOS.; anti-HISTIDINE TAG antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AD1.1.10
Form/Format
Alk. Phos.
Purified IgG conjugated to Alkaline Phosphatase - liquid
Purified IgG conjugated to Alkaline Phosphatase - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Applicable Applications for anti-HISTIDINE TAG antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 1/20; Maximum Dilution: 1/1000;
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
PAX6 transcription factor linked to histidine tag
Fusion Partners
Fusion Partners
Target Species
Synthetic Peptide
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 (MBS212126D800GA). Visualised using a LI-COR Odyssey infrared imaging system)
Testing Data
(Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 (MBS212126D800GA). Visualised using a LI-COR Odyssey infrared imaging system)
Testing Data
(Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody (MBS210678))
Testing Data
(Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody (MBS210678))
Testing Data
(Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (MBS216898) visualised with Rabbit anti Mouse IgG : HRP (MBS235192))
Testing Data
(Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (MBS216898) visualised with Rabbit anti Mouse IgG : HRP (MBS235192))
Testing Data
(His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag (MBS212126))
Testing Data
(His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag (MBS212126))
Related Product Information for anti-HISTIDINE TAG antibody
Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
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Product Notes
The HISTIDINE TAG (Catalog #AAA219367) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HISTIDINE TAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: Minimum Dilution: 1/20; Maximum Dilution: 1/1000; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000. Researchers should empirically determine the suitability of the HISTIDINE TAG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HISTIDINE TAG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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