Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit | ADAM12 elisa kit

Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

Cat. Num. AAA2023202

• Gene Names: ADAMTS12; PRO4389
• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 12 (ADAM12); Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit; MCMP; MCMPMltna; MLTN; MLTNA; Meltrin Alpha; Disintegrin and metalloproteinase domain-containing protein 12; ADAM12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 12 (ADAM12).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 12 (ADAM12) and analogues was observed.
• Sequence Length: 1594

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 12 (ADAM12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 12 (ADAM12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 12 (ADAM12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 12 (ADAM12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADAM12 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 51558724
• NCBI GeneID: 81792
• NCBI Accession #: NP_112217.2
• NCBI GenBank Nucleotide #: NM_030955.2
• Molecular Weight: 168,460 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 12 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 12
• NCBI Official Symbol: ADAMTS12
• NCBI Official Synonym Symbols: PRO4389
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 12
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 12
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAMTS12
• UniProt Synonym Gene Names: ADAM-TS 12; ADAM-TS12; ADAMTS-12
• UniProt Entry Name: ATS12_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS-1) motif. Individual members of this family differ in the number of C-terminal TS-1 motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains eight TS-1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS12: Metalloprotease that may play a role in the degradation of COMP. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: cell migration; proteolysis involved in cellular protein catabolic process; cell-matrix adhesion; negative regulation of chondrocyte differentiation; regulation of inflammatory response; proteoglycan catabolic process