Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit | ADAM10 elisa kit

Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

Cat. Num. AAA2019893

• Gene Names: ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10
• Reactivity: Rat
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Rat A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit; CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; ADAM10 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.
• Sequence Length: 1103

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 78.12-5000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM10 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 10 (ADAM10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 10 (ADAM10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADAM10 elisa kit

Enzyme & Kinase; Tumor immunity; Infection immunity; Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 537544566
• NCBI GeneID: 81794
• NCBI Accession #: NP_112219.3
• NCBI GenBank Nucleotide #: NM_030957.3
• Molecular Weight: 62,530 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 10
• NCBI Official Symbol: ADAMTS10
• NCBI Official Synonym Symbols: WMS; WMS1; ADAM-TS10; ADAMTS-10
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 10
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAMTS10
• UniProt Synonym Gene Names: ADAM-TS 10; ADAM-TS10; ADAMTS-10
• UniProt Entry Name: ATS10_HUMAN

NCBI Description

This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS10: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues. Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1). WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma.

Protein type: Protease; Secreted; EC 3.4.24.-; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular matrix; microfibril

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis

Disease: Weill-marchesani Syndrome 1