Goat anti-Human RNF34 Polyclonal Antibody | anti-RNF34 antibody

RNF34, NT (E3 Ubiquitin-protein Ligase RNF34, Caspase Regulator CARP1, Caspases-8 and -10-associated RING Finger Protein 1, CARP-1, FYVE-RING Finger Protein Momo, Human RING Finger Homologous to Inhibitor of Apoptosis Protein, hRFI, RING Finger Protein 34

Cat. Num. AAA646965

• Gene Names: RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RNF34; Polyclonal Antibody; NT (E3 Ubiquitin-protein Ligase RNF34; Caspase Regulator CARP1; Caspases-8 and -10-associated RING Finger Protein 1; CARP-1; FYVE-RING Finger Protein Momo; Human RING Finger Homologous to Inhibitor of Apoptosis Protein; hRFI; RING Finger Protein 34; Anti -RNF34; anti-RNF34 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human RNF34. Species sequence homology: bovine, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-RNF34 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml
• Immunogen: Synthetic peptide KAGATSMWASCC from the N-terminal of human RNF34.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with RNF34 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with RNF34 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-RNF34 antibody

Ring Finger homologous to Inhibitor of apoptosis protein (hRFI), an anti-apoptotic protein which has been shown to be a novel modulator of NF-kB activation, leading to the upregulation of Bcl2 and BclXL. It is also thought to possess E3 ubiquitin protein ligase activity and has been shown to target caspases 8 and 10 for proteasomal degradation. hRFI is expressed ubiquitously, and has been localized to both the nucleus and the cytoplasm. hRFI is also expressed in oesophageal, gastric and colorectal cancers, suggesting a possible association between hRFI and the development of digestive tract cancers.

Product Categories/Family for anti-RNF34 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 48146801
• NCBI GeneID: 80196
• UniProt Accession #: Q969K3; Q8NG47; Q9H6W8; B7Z933
• Molecular Weight: 41,641 Da
• NCBI Official Full Name: RNF34
• NCBI Official Synonym Full Names: ring finger protein 34, E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF34
• NCBI Official Synonym Symbols: RFI; RIF; RIFF; hRFI; CARP1; CARP-1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF34; caspase regulator CARP1; RING finger protein RIFF; FYVE-RING finger protein MOMO; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF34
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF34
• UniProt Synonym Gene Names: CARP-1; hRFI
• UniProt Entry Name: RNF34_HUMAN

NCBI Description

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate. Ref.1 Ref.8 Ref.9

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with DNAJA3

Potential. Interacts with CASP8 and CASP10. Ref.1 Ref.8

Subcellular location: Nucleus speckle. Endomembrane system; Peripheral membrane protein Ref.1 Ref.8.

Tissue specificity: Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Ref.1 Ref.8

Post-translational modification: Auto-ubiquitinated (in vitro)

By similarity.Proteolytically cleaved by caspases upon induction of apoptosis by TNF. Ref.1

Sequence similarities: Contains 1 FYVE-type zinc finger.Contains 1 RING-type zinc finger.Contains 2 SAP domains.

Caution: Was isolated as potential interaction partner of the mitochondrial protein DNAJA3, but the 2 proteins are not in the same cellular compartments.

Sequence caution: The sequence BAH14169.1 differs from that shown. Reason: Intron retention.