Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " Serum Free" - showing 100-150
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD10:Azide Free (MBS210164))
CD10, Monoclonal Antibody (Cat# AAA210725)
Full Name
MOUSE ANTI HUMAN CD10:FITC
Gene Names
MME; NEP; SFE; CD10; CALLA
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Activity Data
(VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGF121. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. The result was shown in Figure 2. It was obvious that VEGF121 significantly increased cell viability of ECV-304 cells.)
Vascular Endothelial Growth Factor 121 (VEGF121), Active Protein (Cat# AAA2097243)
Full Name
Active Vascular Endothelial Growth Factor 121 (VEGF121)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Testing Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and RPE conjugated Mouse anti Human CD45 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)
CD45, Monoclonal Antibody (Cat# AAA225670)
Full Name
Rat Anti Human CD45: RPE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Nonesterified Free Fatty Acids, Assay Kit (Cat# AAA2548409)
Full Name
Nonesterified Free Fatty Acids Assay Kit
Pricing
$515/96 Tests
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin (MBS210307))
CD59, Monoclonal Antibody (Cat# AAA213548)
Full Name
MOUSE ANTI HUMAN CD59:RPE
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD8:FITC (MBS210635))
CD8, Monoclonal Antibody (Cat# AAA210007)
Full Name
MOUSE ANTI HUMAN CD8:APC
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
IL-4, Recombinant Antibody (Cat# AAA488132)
Full Name
Anti-IL-4 [11B11]
Gene Names
Il4; Il-4; BSF-1
Reactivity
Mouse
Applications
EIA, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:Alexa Fluor 647 (MBS212497A647))
BLTR, Monoclonal Antibody (Cat# AAA215507)
Full Name
MOUSE ANTI HUMAN BLTR:FITC
Gene Names
LTB4R; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
Applications
FC/FACS
Pricing
$215/0.025 mg | $815/5x0.025 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD8:FITC (MBS210635))
CD8, Monoclonal Antibody (Cat# AAA214848)
Full Name
MOUSE ANTI HUMAN CD8:APC
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
FC/FACS
Pricing
$240/25 Tests | $930/5x25 Tests
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)
CD11b, Monoclonal Antibody (Cat# AAA211057)
Full Name
MOUSE ANTI HUMAN CD11b:FITC
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig SWC5 detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
SWC5, Monoclonal Antibody (Cat# AAA225692)
Full Name
Mouse Anti Pig SWC5
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Activity Data
(PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.)
Platelet Derived Growth Factor Subunit A (PDGFA), Active Protein (Cat# AAA2097229)
Full Name
Active Platelet Derived Growth Factor Subunit A (PDGFA)
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $495/0.05 mg | $765/0.1 mg | $945/0.2 mg | $1,845/0.5 mg | $2,730/1 mg | $6,670/5 mg
Immunofluorescence (IF)
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Ly-6C, Monoclonal Antibody (Cat# AAA225659)
Full Name
Rat Anti Mouse Ly-6C: FITC
Gene Names
Ly6c2; Ly-6C2; Ly-6C.2
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using NARG1 antibody.)
NARG1, Polyclonal Antibody (Cat# AAA9604881)
Full Name
NARG1 Antibody
Gene Names
NAA15; Ga19; NATH; TBDN; MRD50; NARG1; NAT1P; TBDN100
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Goat anti-Llama IgG (H&L), Biotin Conjugate Test Data
IgG (H&L), Secondary Antibody (Cat# AAA687389)
Full Name
Goat anti-Llama IgG (H&L) - Affinity Pure, Biotin Conjugate
Reactivity
Llama.
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins
Cross Reactivity: Based on IEP, no reactivity is observed to non-immunoglobulin llama serum immunoglobulins
Applications
EIA, IHC
Purity
Affinity purified antibody is > 95% based on SDS-PAGE
Affinity purified using solid phase Llama IgG.
Affinity purified using solid phase Llama IgG.
Pricing
$335/1 mg | $1,355/5x1 mg
IL-4, Recombinant Antibody (Cat# AAA488131)
Full Name
Anti-IL-4 [11B11]
Reactivity
Mouse
Applications
EIA, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (MBS178867).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysate,Lane 2: human 22RV1 cell lysate,Lane 3: rat spleen tissue lysate,Lane 4: rat thymus tissue lysate,Lane 5: mouse spleen tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD44 at approximately 88KD. The expected band size for CD44 is at 82KD. )
CD44, Polyclonal Antibody (Cat# AAA178867)
Full Name
Anti-CD44 Picoband antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis on HuvEc cell lysate using NARG1 Antibody)
NARG1, Polyclonal Antibody (Cat# AAA9600399)
Full Name
NARG1 Antibody
Gene Names
NAA15; Ga19; NATH; TBDN; MRD50; NARG1; NAT1P; TBDN100
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(FGF13 (Fibroblast growth factor 13) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF13 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF13. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF13 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF13 for 48h. The result was shown in Figure 2. It was obvious that FGF13 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 13 (FGF13), Active Protein (Cat# AAA2097308)
Full Name
Active Fibroblast Growth Factor 13 (FGF13)
Gene Names
Fgf13; FGF-13
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
Activity Data
(FGF23 (Fibroblast growth factor 23) is a member of the fibroblast growth factor family, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF23 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF23. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF23 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF23 for 48h. The result was shown in Figure 2. It was obvious that FGF23 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 23 (FGF23), Active Protein (Cat# AAA2097280)
Full Name
Active Fibroblast Growth Factor 23 (FGF23)
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$165/0.01 mg | $310/0.05 mg | $480/0.1 mg | $585/0.2 mg | $1,120/0.5 mg | $1,660/1 mg | $4,075/5 mg
Activity Data
(FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF22. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. The result was shown in Figure 2. It was obvious that FGF22 significantly increased cell viability of 3T3 cells.)
Fibroblast Growth Factor 22 (FGF22), Active Protein (Cat# AAA2097307)
Full Name
Active Fibroblast Growth Factor 22 (FGF22)
Gene Names
Fgf22; FGF-22; 2210414E06Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Testing Data
(Figure A. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. Pacific Blue conjugated Mouse anti Human CD3 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD55 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
CD55, Monoclonal Antibody (Cat# AAA225631)
Full Name
Mouse Anti Human CD55: FITC
Gene Names
CD55; CR; TC; DAF; CROM; CHAPLE
Reactivity
Human
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD8:FITC)
CD8, Monoclonal Antibody (Cat# AAA210635)
Full Name
MOUSE ANTI HUMAN CD8:FITC
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Activity Data
(Figure 1. Cell proliferation of ECV-304 cells after stimulated with EG-VEGF.Endothelial gland-derived VEGF (EG-VEGF) is an angiogenic protein that is structurally unrelated to VEGF. It is expressed in steroidogenic tissues such as adrenal gland, ovary, testis, and placenta. Like VEGF it can induce fenestrae in endothelial cells. To test the effect of EG-VEGF on cell proliferation of ECV-304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EG-VEGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.(A) Unstimulated ECV-304 cells cultured in 1640 for 96h;(B) ECV-304 cells cultured in 1640, stimulated with 10ng/mL VEGF121 for 96h.)
Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF), Active Protein (Cat# AAA2105050)
Full Name
Active Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)
Gene Names
PROK1; PK1; PRK1; EGVEGF
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
CD29, Monoclonal Antibody (Cat# AAA225641)
Full Name
Mouse Anti Human CD29: FITC
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human, Mink
Does not react with: Rat, Mouse
Does not react with: Rat, Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD4, Monoclonal Antibody (Cat# AAA225685)
Full Name
Mouse Anti Pig CD4: FITC
Reactivity
Pig
Applications
FC/FACS, IF
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human KIR: Low Endotoxin (MBS211215))
KIR, Monoclonal Antibody (Cat# AAA210899)
Full Name
MOUSE ANTI HUMAN KIR:FITC
Gene Names
KIR2DS4; KKA3; KIR1D; NKAT8; CD158I; KIR412; NKAT-8; KIR2DS1
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD8 . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
CD8, Monoclonal Antibody (Cat# AAA225633)
Full Name
Rat Anti Mouse CD8: FITC
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RA:RPE)
CD45RA, Monoclonal Antibody (Cat# AAA213889)
Full Name
MOUSE ANTI HUMAN CD45RA:RPE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Alexa Fluor 647 (MBS211993A647))
CD38, Monoclonal Antibody (Cat# AAA210303)
Full Name
MOUSE ANTI HUMAN CD38:Biotin
Gene Names
CD38; T10; ADPRC 1
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Staining of stimulated rat spleen cells with Rat anti Mouse CD134:Azide Free (MBS210149))
CD134, Monoclonal Antibody (Cat# AAA210686)
Full Name
RAT ANTI MOUSE CD134:FITC
Gene Names
Tnfrsf4; Ox40; ACT35; CD134; Ly-70; Txgp1; TXGP1L
Applications
FC/FACS
Pricing
$630/0.5 mg | $2,680/5x0.5 mg
Activity Data
(Figure. The chemotactic effect of CX3CL1 on THP-1 cells.Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (1ng/mL, 10ng/mL, 100ng/mL and 1000ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 1-1000ng/mL.(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 10ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h;(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.)
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Active Protein (Cat# AAA2105056)
Full Name
Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Gene Names
CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$210/0.01 mg | $440/0.05 mg | $675/0.1 mg | $835/0.2 mg | $1,620/0.5 mg | $2,415/1 mg | $5,900/5 mg
Sequence
Endostatin (ES), Active Protein (Cat# AAA2105098)
Full Name
Active Endostatin (ES)
Gene Names
NPPB; BNP; BNP1
Reactivity
Homo sapiens (Human)
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$205/0.01 mg | $405/0.05 mg | $625/0.1 mg | $770/0.2 mg | $1,495/0.5 mg | $2,225/1 mg | $5,435/5 mg
Activity Data
(Figure. Cell proliferation of ECV-304 cells after stimulated with VEGFA.Vascular endothelial growth factor A (VEGFA) is a protein that in humans is encoded by the VEGFA gene. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. VEGFA shows prominent activity with vascular endothelial cells, primarily through its interactions with the VEGFR1 and -R2 receptors found in prominently on the endothelial cell membrane. Thus, proliferation assay of recombinant human VEGFA was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of VEGFA. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with VEGFA for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with human recombinant VEGFA for 48h. The result was shown in Figure 2. It was obvious that VEGFA significantly increased cell viability of ECV-304 cells.(A) ECV-304 cells cultured in 1640, stimulated with 100ng/mL VEGFA for 48h; (B) Unstimulated ECV-304 cells cultured in 1640 for 48h.)
Vascular Endothelial Growth Factor A (VEGFA), Active Protein (Cat# AAA2105083)
Full Name
Active Vascular Endothelial Growth Factor A (VEGFA)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $480/0.05 mg | $740/0.1 mg | $915/0.2 mg | $1,790/0.5 mg | $2,645/1 mg | $6,465/5 mg
Activity Data
(Figure. The chemotactic effect of CX3CL1 on THP-1 cellsChemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150uL cell suspension, 106 cells/mL in RPMI 1640 with FBS free) and SLC (15.625ng/mL, 31.25ng/mL, 62.5ng/mL and 125ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37 degree C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated Jurkat cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical results were shown in Figure 2. The optimum chemotaxis of CX3CL1 occurs at 15.625-125ng/mL.(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 31.25ng/mL CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h; (B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without CX3CL1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 1h.)
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Active Protein (Cat# AAA2105057)
Full Name
Active Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Gene Names
Cx3cl1; Cx3c; Scyd1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$180/0.01 mg | $345/0.05 mg | $530/0.1 mg | $650/0.2 mg | $1,260/0.5 mg | $1,870/1 mg | $4,595/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PODXL using anti-PODXL antibody (MBS1750830).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse kidney tissue lysates,Lane 2: mouse kidney tissue lysates,Lane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PODXL antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PODXL at approximately 58KD. The expected band size for PODXL is at 58KD. )
PODXL, Polyclonal Antibody (Cat# AAA1750830)
Full Name
Anti-PODXL Picoband Antibody
Gene Names
PODXL; PC; PCLP; Gp200; PCLP-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Azide Free (MBS210176))
CD8, Monoclonal Antibody (Cat# AAA213666)
Full Name
RAT ANTI MOUSE CD8:RPE
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 (MBS213089), red in A and Mouse anti Human CD21 (MBS212051), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
CD43, Monoclonal Antibody (Cat# AAA213855)
Full Name
MOUSE ANTI HUMAN CD43:RPE
Gene Names
SPN; LSN; CD43; GALGP; GPL115
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC (MBS211057))
CD11b, Monoclonal Antibody (Cat# AAA216466)
Full Name
MOUSE ANTI HUMAN CD11b:FITC
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Activity Data
(IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.)
Interleukin 20 (IL20), Active Protein (Cat# AAA2097236)
Full Name
Active Interleukin 20 (IL20)
Gene Names
IL20; IL-20; IL10D; ZCYTO10
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $675/0.2 mg | $1,305/0.5 mg | $1,940/1 mg | $4,765/5 mg
Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody (MBS213189) followed by HRP-polymer detection and DAB substrate development (low power) following antigen retrieval using citrate buffer at pH6.2)
CD44, Monoclonal Antibody (Cat# AAA213890)
Full Name
MOUSE ANTI HUMAN CD44:RPE
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Cryptosporidium, Monoclonal Antibody (Cat# AAA375013)
Full Name
Mouse Anti-Cryptosporidium [B-G2.7]
Applications
EIA, IF
Purity
Protein A affinity chromatography of serum free tissue culture supernatent
Pricing
$470/0.1 mL | $1,960/5x0.1 mL
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD8:FITC (MBS210635))
CD8, Monoclonal Antibody (Cat# AAA214850)
Full Name
MOUSE ANTI HUMAN CD8:FITC
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
FC
Pricing
$195/0.025 mg | $730/5x0.025 mg
Testing Data
(Staining of stimulated rat spleen cells with Rat anti Mouse CD134:Azide Free (MBS210149))
CD134, Monoclonal Antibody (Cat# AAA210684)
Full Name
RAT ANTI MOUSE CD134:FITC
Gene Names
Tnfrsf4; Ox40; ACT35; CD134; Ly-70; Txgp1; TXGP1L
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
CD81, Monoclonal Antibody (Cat# AAA225635)
Full Name
Mouse Anti Human CD81: RPE
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Sheep, Goat
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data #1
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45RO (MBS213021))
CD45RO, Monoclonal Antibody (Cat# AAA213831)
Full Name
MOUSE ANTI HUMAN CD45RO:RPE
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Flow Cytometry
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB)
(Western Blot: Sample: Human Serum.)
Alpha-1-Antitrypsin (a1AT), Polyclonal Antibody (Cat# AAA2001932)
Full Name
Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT)
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT
Reactivity
Human
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
SDS-Page
(Recombinant Human NRG1-alpha/HRG1-alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 8 kDa.)
NRG1-alpha/HRG1-alpha, Active Protein (Cat# AAA9139658)
Full Name
Recombinant Human NRG1-alpha/HRG1-alpha Protein
Gene Names
NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2
Purity
>95% by SDS-PAGE.
Pricing
$155/0.01 mg | $185/0.02 mg | $245/0.05 mg | $325/0.1 mg | $470/0.2 mg | $135/0.005 mg | $1,965/5x0.2 mg
SDS-Page
VEGF, Active Protein (Cat# AAA203390)
Full Name
VEGF, 205-324aa, Mouse, His tag, E Coli (Bioactivity Validated)
Gene Names
Vegfa; Vpf; Vegf
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$230/0.01 mg | $405/0.05 mg | $985/0.25 mg | $4,180/5x0.25 mg
Activity Data
(Figure. Cell proliferation of A549 cells after stimulated with GREM1.Gremlin (GREM1) is an inhibitor in the TGF beta signaling pathway. GREM1 and other BMP antagonists are important in the survival of cancer stroma survival and proliferation in some cancers. The protein expression is found in many cancers and is thought to play important roles in uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma carcinomas. A proliferation assay was conducted to detect the bioactivity of recombinant mouse GREM1 using A549 cells. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM prior to the addition of various concentrations of GREM1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly,10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of A549 cells after incubation with GREM1 for 96h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GREM1 for 96h. The result was shown in Figure 2. It was obvious that GREM1 significantly increased cell viability of A549 cells.(A) A549 cells cultured in DMEM, stimulated with 1ng/mL GREM1 for 96h; (B) Unstimulated A549 cells cultured in DMEM for 96h.)
Gremlin 1 (GREM1), Active Protein (Cat# AAA2105166)
Full Name
Active Gremlin 1 (GREM1)
Gene Names
Grem1; ld; Drm; Grem; Cktsf1b1
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$205/0.01 mg | $405/0.05 mg | $625/0.1 mg | $770/0.2 mg | $1,495/0.5 mg | $2,225/1 mg | $5,435/5 mg
