WB (Western Blot)
(Detection of human Senataxin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, SW620, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Senataxin Recombinant Antibody | SETX recombinant antibody
Rabbit anti-Senataxin Recombinant Monoclonal Antibody [BLR050F]
Gene Names
SETX; ALS4; AOA2; Sen1; SCAR1; bA479K20.2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Senataxin, Recombinant Antibody; Rabbit anti-Senataxin Recombinant Monoclonal Antibody [BLR050F]; SCAR1; probable helicase senataxin; Senataxin; SEN1 homolog; bA479K20.2; ALS4; AOA2; amyotrophic lateral sclerosis 4 protein; Sen1; Probable helicase senataxin; senataxin; SETX recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR050F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for SETX recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 900 and 950
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Senataxin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, SW620, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Senataxin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, SW620, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used for IP at 6 ul/mg lysate. Senataxin was also immunoprecipitated by a previous lot of this antibody (AAA213548 lot 1) and a second antibody against a different epitope of Senataxin . For blotting immunoprecipitated Senataxin, AAA213548 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Senataxin recombinant monoclonal antibody (AAA213548 lot 2) used for IP at 6 ul/mg lysate. Senataxin was also immunoprecipitated by a previous lot of this antibody (AAA213548 lot 1) and a second antibody against a different epitope of Senataxin . For blotting immunoprecipitated Senataxin, AAA213548 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of human Senataxin (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-Senataxin recombinant monoclonal (AAA213548) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Senataxin (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-Senataxin recombinant monoclonal (AAA213548) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for SETX recombinant antibody
Mutations in Senataxin result in the neurodegenerative disorders Ataxia-Oculomotor Apraxia 2 (AOA2) and amyotrophic lateral sclerosis (ALS4). Senataxin bears an RNA/DNA helicase domain suggesting a role in DNA and RNA processing and has recently been found to be involved in the DNA damage response.
Product Categories/Family for SETX recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
306,341 Da
NCBI Official Full Name
probable helicase senataxin isoform 1
NCBI Official Synonym Full Names
senataxin
NCBI Official Symbol
SETX
NCBI Official Synonym Symbols
ALS4; AOA2; Sen1; SCAR1; bA479K20.2
NCBI Protein Information
probable helicase senataxin
UniProt Protein Name
Probable helicase senataxin
UniProt Gene Name
SETX
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Product Notes
The SETX setx (Catalog #AAA213548) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Senataxin Recombinant Monoclonal Antibody [BLR050F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Senataxin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SETX setx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Senataxin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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