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Testing Data (Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Mouse CD81 Monoclonal Antibody | anti-CD81 antibody

Mouse Anti Human CD81: RPE

Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Sheep, Goat
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CD81; Monoclonal Antibody; Mouse Anti Human CD81: RPE; CD81 antibody (1D6); Mouse anti Human CD81; anti-CD81 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Chimpanzee, Sheep, Goat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D6
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
236
Applicable Applications for anti-CD81 antibody
Flow Cytometry (FC/FACS)
Immunogen
OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
Conjugation
RPE
Carrier Free
Yes
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Testing Data (Figure A. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE-Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 488 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and Alexa Fluor 647 conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data

(Figure A. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data (Figure A. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD19 and RPE conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Testing Data

(Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.)

Testing Data (Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Human CD19 and FITC conjugated Mouse anti Human CD81 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for anti-CD81 antibody
Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation. Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
975
UniProt Accession #
NCBI Official Full Name
CD81 antigen
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
Protein Family
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.

Research Articles on CD81

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Product Notes

The CD81 cd81 (Catalog #AAA225635) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD81: RPE reacts with Human, Chimpanzee, Sheep, Goat and may cross-react with other species as described in the data sheet. AAA Biotech's CD81 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD81 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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