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10000 results for " Ring Finger Protein" - showing 100-150


Western Blot (WB) (Western blotAll lanes: RNF212 antibody at 14ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 34,15,22,32,27,31 kDaObserved band size: 33 kDa)

RNF212, Polyclonal Antibody (Cat# AAA1493373)

Full Name
Rabbit anti-human RNF212 polyclonal Antibody
Gene Names
RNF212; ZHP3
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB) (Western blot detection of c-Cbl in C6, Jurkat, K562, 3T3 and Hela cell lysates using c-Cbl mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.)

c-Cbl, Monoclonal Antibody (Cat# AAA476198)

Full Name
Anti-c-Cbl
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Applications
WB, IP, IHC-P, ICC/IF
Purity
Ascitic Fluid
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (TRIM34 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM34 antibody detected the TRIM34 protein (arrow).)

TRIM34, Polyclonal Antibody (Cat# AAA9207343)

Full Name
TRIM34 Antibody (N-term)
Gene Names
TRIM34; IFP1; RNF21
Reactivity
Human
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blotAll lanes: RFPL2 antibody at 3.41ug/mlLane 1 : U87 whole cell lysateLane 2 : MCF7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 43,33,36 kDaObserved band size: 42 kDa)

RFPL2, Polyclonal Antibody (Cat# AAA7044159)

Full Name
RFPL2 Antibody
Gene Names
RFPL2; RNF79
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB) (Western blotAll lanes: RNF135 antibody at 3.51 ug/mlLane 1: 293T whole cell lysateLane 2: Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 48,23,31 kDaObserved band size: 48 kDa)

RNF135, Polyclonal Antibody (Cat# AAA7046597)

Full Name
RNF135 Antibody
Gene Names
RNF135; L13; MMFD; REUL; Riplet
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL

RNF7, cDNA Clone (Cat# AAA1278272)

Full Name
RNF7 cDNA Clone
Gene Names
RNF7; SAG; ROC2; CKBBP1
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Typical Testing Data/Standard Curve (for reference only)

HLTF, ELISA Kit (Cat# AAA2512719)

Full Name
Mouse HLTF (Helicase Like Transcription Factor) ELISA Kit
Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Mouse
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells

TRIM11, cDNA Clone (Cat# AAA1275134)

Full Name
TRIM11 cDNA Clone
Gene Names
TRIM11; BIA1; RNF92
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB) (Western blotAll lanes: RNF135 antibody at 5.88 ug/mlLane 1: 293T whole cell lysateLane 2: Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 48,23,31 kDaObserved band size: 48 kDa)

RNF135, Polyclonal Antibody (Cat# AAA7046087)

Full Name
RNF135 Antibody
Gene Names
RNF135; L13; MMFD; REUL; Riplet
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Western Blot (WB) (Western blotAll lanes: RNF5 antibody at 3.94 ug/ml+ Mouse gonadal tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 20 kDaObserved band size: 20 kDa)

RNF5, Polyclonal Antibody (Cat# AAA7045423)

Full Name
RNF5 Antibody
Gene Names
RNF5; RMA1; RING5
Reactivity
Human, Mouse
Applications
EIA, WB, IF, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL

TRAIP, cDNA Clone (Cat# AAA1275597)

Full Name
TRAIP cDNA Clone
Gene Names
TRAIP; TRIP; SCKL9; RNF206
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB) (Western blotAll lanes: TRIM23 antibody at 4.55 ug/ml+ Raw264.7 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 65,64,62 kDaObserved band size: 64,35 kDa)

TRIM23, Polyclonal Antibody (Cat# AAA7046329)

Full Name
TRIM23 Antibody
Gene Names
TRIM23; ARD1; ARFD1; RNF46
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA214958)

Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg

TRIM39, cDNA Clone (Cat# AAA1273478)

Full Name
TRIM39 cDNA Clone
Gene Names
TRIM39; TFP; RNF23; TRIM39B
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB) (Western blot analysis of Hela whole cell lysates, using UHRF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

UHRF1, Polyclonal Antibody (Cat# AAA9607582)

Full Name
UHRF1 Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, IP, EIA, ChIP
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blotAll lanes: RNF10 antibody at 4ug/ml+Mouse brain tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 90,91 kDaObserved band size: 90 kDa)

RNF10, Polyclonal Antibody (Cat# AAA7001612)

Full Name
RNF10 Antibody
Gene Names
RNF10; RIE2
Reactivity
Human, mouse
Applications
EIA, WB, IHC
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA210664)

Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)

HLTF, ELISA Kit (Cat# AAA2511303)

Full Name
Human HLTF (Helicase Like Transcription Factor) ELISA Kit
Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells

RNF185, cDNA Clone (Cat# AAA1273691)

Full Name
RNF185 cDNA Clone
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MID1 antibody (MBS9127519) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

MID1, Polyclonal Antibody (Cat# AAA9127519)

Full Name
MID1 Polyclonal Antibody
Gene Names
MID1; OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18
Applications
WB, IF, ICC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF) (F6318 staining HEPG2 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

PML, Polyclonal Antibody (Cat# AAA9606977)

Full Name
PML Antibody
Gene Names
PML; MYL; RNF71; PP8675; TRIM19
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9605483 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

RNF144A, Polyclonal Antibody (Cat# AAA9605483)

Full Name
RNF144A Antibody
Gene Names
RNF144A; hUIP4; RNF144; UBCE7IP4
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page

TRAF-interacting protein (TRAIP), Recombinant Protein (Cat# AAA1380981)

Full Name
Recombinant Human TRAF-interacting protein (TRAIP)
Gene Names
TRAIP; TRIP; SCKL9; RNF206
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $510/0.1 mg (E-Coli) | $310/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
Testing Data (Sample(30 ug whole cell lysate)A: Hep G2 10% SDS PAGEPrimary antibody diluted at 1: 1000)

MURF1, Polyclonal Antibody (Cat# AAA9411532)

Full Name
MURF1 antibody
Gene Names
TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by antigen-affinity chromatography.
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts from 293 and rat brain, using RNF130 Antibody.)

RNF130, Polyclonal Antibody (Cat# AAA9606108)

Full Name
RNF130 Antibody
Gene Names
RNF130; GP; G1RZFP; GOLIATH
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Western blot analysis of immunized recombinant protein, using anti-CBLB monoclonal antibody. )

CBLB, Monoclonal Antibody (Cat# AAA120301)

Full Name
Mouse monoclonal antibody Anti-Human CBLB
Gene Names
CBLB; Cbl-b; RNF56; Nbla00127
Reactivity
Human
Applications
WB
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB) (Western blot analysis of MARCH7 using HuvEc whole cell lysates)

MARCH7, Polyclonal Antibody (Cat# AAA9609800)

Full Name
MARCH7 Antibody
Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of RING1b expression in Jurkat (A), SHSY5Y (B), mouse testis (C) whole cell lysates.)

RING1b, Polyclonal Antibody (Cat# AAA8245837)

Full Name
Anti-RING1b Antibody
Gene Names
RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, ChIP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BRCA1 Monoclonal Antibody (BRCA1/1398).)

BRCA1, Monoclonal Antibody (Cat# AAA4380238)

Full Name
BRCA1 (Breast Marker)
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot detection of c-IAP2 in Ramos, COS7, Raji and Daudi cell lysates using c-IAP2 mouse mAb (1:1000 diluted).Predicted band size: 68KDa, Observed band size:72KDa.)

cIAP2, Monoclonal Antibody (Cat# AAA475100)

Full Name
Anti-cIAP2 Mouse mAb
Gene Names
BIRC3; AIP1; API2; MIHC; CIAP2; HAIP1; HIAP1; MALT2; RNF49; c-IAP2
Reactivity
Human, Monkey
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)

ZFP36L2, Polyclonal Antibody (Cat# AAA5400849)

Full Name
ZFP36L2 Antibody
Gene Names
ZFP36L2; BRF2; ERF2; ERF-2; TIS11D; RNF162C
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, IP, WB
Purity
Immobilized antigen affinity chromatography
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC) (MBS9606827 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

BIRC2, Polyclonal Antibody (Cat# AAA9606827)

Full Name
BIRC2 Antibody
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinty purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9604804 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

TRIM59, Polyclonal Antibody (Cat# AAA9604804)

Full Name
TRIM59 Antibody
Gene Names
TRIM59; MRF1; TSBF1; IFT80L; RNF104; TRIM57
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Anti-BIRC7 / Livin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

BIRC7 / Livin, Polyclonal Antibody (Cat# AAA240476)

Full Name
Rabbit Polyclonal to Human BIRC7 / Livin
Gene Names
BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Peptide Column Affinity Chromatography
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)

BMI1, Monoclonal Antibody (Cat# AAA4381250)

Full Name
BMI1 (Oncogene and Stem Cell Marker)
Gene Names
BMI1; PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
Reactivity
Human, Mouse
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA216888)

Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Immunofluorescence (IF) (MBS9607951 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

RAD18, Polyclonal Antibody (Cat# AAA9607951)

Full Name
RAD18 Antibody
Gene Names
RAD18; RNF73
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using RNF144B antibody.)

RNF144B, Polyclonal Antibody (Cat# AAA9605484)

Full Name
RNF144B Antibody
Gene Names
RNF144B; PIR2; IBRDC2; p53RFP; bA528A10.3
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from Jurkat, A549, MCF7 and C6 cell lysates using Ring1A mouse mAb (1:3000 diluted).Predicted band size:42KDa.Observed band size:54KDa.)

Ring1A, Monoclonal Antibody (Cat# AAA476692)

Full Name
Anti-Ring1A Mouse mAb
Gene Names
RING1; RNF1; RING1A
Reactivity
Human, Rat
Applications
WB, IF
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot-RNF149 Polyclonal Antibody)

RNF149, Polyclonal Antibody (Cat# AAA9140394)

Full Name
RNF149 Polyclonal Antibody
Gene Names
RNF149; DNAPTP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western Blot: Sample: Rat Serum.)

Brain Finger Protein, Polyclonal Antibody (Cat# AAA2027110)

Full Name
Polyclonal Antibody to Brain Finger Protein (BFP)
Gene Names
Rnf112; BFP; Bfb; Zfp179; Znf179
Reactivity
Rat
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $355/0.5 mL | $435/1 mL | $1,240/5x1 mL
Western Blot (WB) (Western blot analysis of extracts of mouse liver, using TRIM69 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

TRIM69, Polyclonal Antibody (Cat# AAA9126562)

Full Name
TRIM69 Polyclonal Antibody
Gene Names
TRIM69; Trif; HSD34; RNF36; HSD-34
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from rat brain, using PJA1 Antibody.)

PJA1, Polyclonal Antibody (Cat# AAA9609804)

Full Name
PJA1 Antibody
Gene Names
PJA1; RNF70; PRAJA1
Reactivity
Human, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of MUL1 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

MUL1, Polyclonal Antibody (Cat# AAA9604103)

Full Name
MUL1 Antibody
Gene Names
MUL1; GIDE; MAPL; MULAN; RNF218; C1orf166
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RNF168 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

RNF168, Polyclonal Antibody (Cat# AAA129343)

Full Name
RNF168 Polyclonal Antibody
Gene Names
RNF168; hRNF168
Reactivity
Human
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Fig: 1 Western blot analysis of TRAF5 in normal human tissues using at 1: 2000. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 degradation fragments.)

TRAF-5, Polyclonal Antibody (Cat# AAA668819)

Full Name
Polyclonal Antibody to TRAF-5
Gene Names
TRAF5; RNF84; MGC:39780
Reactivity
Rat, Mouse, Human
Applications
IP, IHC, WB
Purity
Serum
Pricing
$300/0.05 mL | $1,280/5x0.05 mL
Immunofluorescence (IF) (MBS9600490 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

RNF144A, Polyclonal Antibody (Cat# AAA9600490)

Full Name
RNF144A Antibody
Gene Names
RNF144A; hUIP4; RNF144; UBCE7IP4
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

Protein PML (PML), Recombinant Protein (Cat# AAA962061)

Full Name
Recombinant Human Protein PML (PML) , partial
Gene Names
PML; MYL; RNF71; PP8675; TRIM19
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,605/1 mg (E-Coli) | $1,160/0.1 mg (Baculovirus) | $450/0.1 mg (E-Coli) | $465/0.02 mg (Baculovirus) | $265/0.02 mg (E-Coli) | $7,045/5x1 mg (E-Coli) | $5,090/5x0.1 mg (Baculovirus)
Western Blot (WB) (Western blot analysis of CGRRF1 using K562 whole cell lysates)

CGRRF1, Polyclonal Antibody (Cat# AAA9609674)

Full Name
CGRRF1 Antibody
Gene Names
CGRRF1; CGR19; RNF197
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of RNF5 using Jurkat whole cell lysates)

RNF5, Polyclonal Antibody (Cat# AAA9609807)

Full Name
RNF5 Antibody
Gene Names
RNF5; RMA1; RING5
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
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