Rabbit anti-Human BIRC7 / Livin Polyclonal Antibody | anti-BIRC7 antibody

Rabbit Polyclonal to Human BIRC7 / Livin

Cat. Num. AAA240476

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Peptide Column Affinity Chromatography
• Synonyms: BIRC7 / Livin; Polyclonal Antibody; Rabbit Polyclonal to Human BIRC7 / Livin; Anti-BIRC7 / Livin Antibody (aa264-280) IHC-plus; BIRC7; KIAP; Livin inhibitor of apoptosis; MLIAP; ML-IAP; RNF50; RING finger protein 50; LIVIN; Human BIRC7; Livin; anti-BIRC7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 280

• Applicable Applications for anti-BIRC7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (5 ug/ml), WB (2 - 4 ug/ml); Livin antibody can be used for detection of Livin by Western blot at 0.5 ug/mL. A band at 33 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 33 kDa
• Target Species: Human
• Immunogen Description: Synthetic peptide from human BIRC7 / Livin. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Rabbit (93%); Marmoset, Panda, Bovine (86%).
• Family/Subfamily: Apoptosis
• Antigen type: Synthetic Peptide
• Immunogen Species: BIRC7 / Livin antibody was raised agaisnt human
• Immunogen: BIRC7 / Livin antibody was raised agasint 17 AA peptide near the C-Terminus of human livin. The immunogen is located within the last 50 AA of livin
• Antigen Modification: aa264-280
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-BIRC7 / Livin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Livin in Human Small Intestine cells with Livin antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of Livin expression in human Raji cell lysate with Livin antibody at 0.5 ug/ml.)

Related Product Information for anti-BIRC7 antibody

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP.

Product Categories/Family for anti-BIRC7 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 11545910
• NCBI GeneID: 79444
• NCBI Accession #: NP_071444.1
• NCBI GenBank Nucleotide #: NM_022161.3
• UniProt Accession #: Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 7 isoform beta
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7; RING finger protein 50; livin inhibitor of apoptosis; kidney inhibitor of apoptosis protein; melanoma inhibitor of apoptosis protein
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

Uniprot Description

BIRC7: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain. Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. Belongs to the IAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Apoptosis; EC 6.3.2.-; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: Golgi apparatus; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: regulation of signal transduction; protein ubiquitination; activation of JNK activity; regulation of cell proliferation; negative regulation of apoptosis