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1414 results for " Methylated Antibody" - showing 1300-1350
5-Methylated Cytosine (5-methyl cytosine), Polyclonal Antibody (Cat# AAA515930)
Full Name
Anti-5-Methylated Cytosine (5-methyl cytosine)
Reactivity
5-Methylated Cytosine
Applications
WB, IP
Pricing
$580/0.25 mg | $2,380/5x0.25 mg
N6-Methyladenosine, Monoclonal Antibody (Cat# AAA389037)
Full Name
N6-Methyladenosine (m6A) antibody (mAb)(Clone 17-3-4-1)
Reactivity
Budding Yeast, Human
Applications
DB, IP
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $590/0.1 mg | $2,595/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using pan-Mono-MethylR（R)
pan-Mono-MethylR (R*GG) Motif, Polyclonal Antibody (Cat# AAA9143247)
Full Name
pan-Mono-MethylR (R*GG) Motif Rabbit pAb
Reactivity
All
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Dot Blot (DB)
(Dot blot of AbFlex 5-methylcytosine antibody (rAb). DNA standards from (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 ug/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.)
5-methylcytosine, Antibody (Cat# AAA389458)
Full Name
AbFlex 5-methylcytosine antibody (rAb)
Reactivity
Not Species Specific
Applications
DB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Western Blot (WB)
(Sample Data: Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri- epsilon-N-methyl), Biotin conjugated (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional N- epsilon-tri-methyl lysine peptide inhibitor (50 ng/ul) (B).)
Methylated Lysine (Tri-epsilon-N-methyl), Polyclonal Antibody (Cat# AAA516056)
Full Name
Anti-Methylated Lysine (Tri-epsilon-N-methyl), Biotin Conjugated
Reactivity
Pan-specific antibody
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
Applications
WB, EIA, IHC, IF
Purity
By affinity chromatography on a tri-methyl lysine agarose column
Pricing
$435/0.1 mg | $1,735/5x0.1 mg
Western Blot (WB)
(Sample Data: Representative western blot of the tri-methylated protein profile with Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated (1:1000) using human melanoma cells (MMRU) lysate (A) and MMRU lysate plus additional N- epsilon-tri-methyl lysine peptide inhibitor (50 ng/ul) (B).)
Methylated Lysine (Tri-epsilon-N-methyl), Polyclonal Antibody (Cat# AAA516058)
Full Name
Anti-Methylated Lysine (Tri-epsilon-N-methyl), HRP Conjugated
Reactivity
Pan-specific antibody
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
Applications
WB, EIA, IP, IHC, IF
Purity
By affinity chromatography on a tri-methyl lysine agarose column
Pricing
$435/0.1 mg | $1,735/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Pan DiMethyl-lysine Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)
Pan DiMethyl-lysine, Polyclonal Antibody (Cat# AAA9143246)
Full Name
Pan DiMethyl-lysine Rabbit pAb
Reactivity
All
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Dot Blot (DB)
(5-mCpA Antibody Specificity Dot blot analysis was used to confirm the specificity of 5-mCpA antibody for the 5-mCpA dinucleotide. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with 5-mCpA (1 ug/ml dilution). Column 1: 5-meCpA. Column 2: CpA. Column 3: 5-meCpC. Column 4: 5-meCpT. Column 5: 5-meCpG. Column 6: Ap5mC)
5-mCpA, Monoclonal Antibody (Cat# AAA389043)
Full Name
5-mCpA antibody (mAb)(Clone 2C8H8A6)
Reactivity
Not Species Specific
Applications
DB
Purity
Protein A Chromatography
Pricing
$190/0.01 mg | $545/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using pan-TriMethyl-lysine pAb at 1:500 dilution.HeLa and MCF7 cells were treated by ADOX (100 uM) for 24 hours .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
pan-TriMethyl-lysine, Polyclonal Antibody (Cat# AAA9143244)
Full Name
pan-TriMethyl-lysine Rabbit pAb
Reactivity
All
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Histone H3 monomethyl Arg8 pAb tested by Western blot. HeLa cell nuclear extract (20 ug) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).)
Histone H3R8me1, Polyclonal Antibody (Cat# AAA388536)
Full Name
Histone H3R8me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, WB
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
SDS-Page
(AbFlex Histone H3K4me0 antibody (rAb) tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.1 ug/ml.)
Histone H3K4me0, Antibody (Cat# AAA389513)
Full Name
AbFlex Histone H3K4me0 antibody (rAb, rabbit)
Reactivity
Human
Applications
DB, WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
SDS-Page
(AbFlex Histone H3K4me1 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me1 antibody at 0.5 ug/ml. Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for detection of Histone H3K4me1.)
Histone H3K4me1, Antibody (Cat# AAA389500)
Full Name
AbFlex Histone H3K4me1 antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
RPL23A, Polyclonal Antibody (Cat# AAA9630663)
Full Name
RPL23A Antibody
Gene Names
RPL23A; L23A; MDA20
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(Histone H3 pan-methyl Lys9 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H3 pan-methyl Lys9 antibody (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Histone H3K9me1me2me3, Polyclonal Antibody (Cat# AAA388426)
Full Name
Histone H3K9me1me2me3 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Pricing
$200/0.01 mL | $590/0.2 mL | $2,595/5x0.2 mL
Western Blot (WB)
(Histone H3 dimethyl Lys14 pAb tested by Western blot. Detection of dimethylated Histone H3 by Western blot. The analysis was performed using 20 ug HeLa acid extract (10 ug, lane 1) and Histone H3 dimethyl Lys14 pAb at a 1:500 dilution.)
Histone H3K14me2, Polyclonal Antibody (Cat# AAA388452)
Full Name
Histone H3K14me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
WB
Pricing
$200/0.01 mL | $590/0.2 mL | $2,595/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using pan-Tri-Methyl K Motif antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
pan-Tri-Methyl lysine, Polyclonal Antibody (Cat# AAA9143566)
Full Name
pan-Tri-Methyl lysine Rabbit pAb
Reactivity
All
Applications
WB, IP
Purity
Affinity purification
Pricing
$250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunofluorescence (IF)
(Histone H3 monomethyl Lys9 pAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 monomethyl Lys9 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).)
Histone H3K9me1, Polyclonal Antibody (Cat# AAA388429)
Full Name
Histone H3K9me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP-Seq, DB, ICC, IF, WB
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (40 ug) probed with DNMT1 monomethyl Lys142 antibody (1:500).)
DNMT1 monomethyl Lys142, Polyclonal Antibody (Cat# AAA388923)
Full Name
DNMT1 monomethyl Lys142 antibody (pAb)
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K36 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
MonoMethyl-Histone H3-K36, Polyclonal Antibody (Cat# AAA9143741)
Full Name
MonoMethyl-Histone H3-K36 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF, IP, ChIP, ChIP
Purity
Affinity purification
Pricing
$250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3K9me0 Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from PC9, lung adenocarcinoma, cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify Histone H3K9me0 binding sites. The image shows binding across a region of chromosome 17.)
Histone H3K9me0, Antibody (Cat# AAA389451)
Full Name
AbFlex Histone H3K9me0 antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, EIA, ICC, IF, WB
Purity
Ni-NTA
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3K4me2 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of HeLa cell chromatin and 5 ug of AbFlex Histone H3K4me2 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.2 million sequence tags were mapped to identify Histone H3K4me2 binding sites on chromosome 7.)
Histone H3K4me2, Antibody (Cat# AAA389515)
Full Name
AbFlex Histone H3K4me2 antibody (rAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Immunofluorescence (IF)
(Histone H3 dimethyl Lys27 antibody tested by immunofluorescence. HeLa cells stained at 2 ug/ml with Histone H3 dimethyl Lys27 antibody. Top panel: Histone H3 dimethyl Lys27 antibody. Middle panel: DAPI. Bottom panel: merge.)
Histone H3K27me2, Polyclonal Antibody (Cat# AAA388632)
Full Name
Histone H3K27me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $590/0.1 mg | $2,595/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys36 antibody tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from a human lymphoma cell line (3 million cells). The ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows H3K36me2 enrichment across the FMNL2 gene.)
Histone H3K36me2, Polyclonal Antibody (Cat# AAA388342)
Full Name
Histone H3K36me2 antibody (pAb)
Reactivity
Budding Yeast, Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, ICC, IF, WB
Pricing
$200/0.01 mL | $375/0.05 mL | $600/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Histone H3K14me1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with H3K14me1 antibody at a 1:500 dilution.)
Histone H3K14me1, Polyclonal Antibody (Cat# AAA388929)
Full Name
Histone H3K14me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, IF, WB
Purity
Affinity Purified
Pricing
$200/0.01 mL | $590/0.1 mL | $2,595/5x0.1 mL
PGC-1a, Polyclonal Antibody (Cat# AAA9459413)
Full Name
PGCa (Mono-methyl-K224) Antibody
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
Reactivity
Human
Applications
WB
Purity
The antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$355/0.1 mL | $1,440/5x0.1 mL
Chromatin Immunoprecipitation (ChIP)
(Histone H3R8me2a antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a mouse osteoblast cell line and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Histone H3R8me2a, Polyclonal Antibody (Cat# AAA388527)
Full Name
Histone H3R8me2a (asymmetric) antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, WB
Purity
Affinity Purified
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot. Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 ug). Lane 2: Nuclear extract from HeLa cells (20 ug).)
Histone H4R3me2s, Polyclonal Antibody (Cat# AAA388799)
Full Name
Histone H4R3me2s (symmetric) antibody (pAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, WB
Purity
Affinity Purified
Pricing
$200/0.01 mL | $375/0.05 mL | $600/0.1 mL | $2,645/5x0.1 mL
Chromatin Immunoprecipitation (ChIP)
(Histone H3K27me2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 2 ug of Histone H3K27me2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using primer pairs specific for the Myt1-9 gene, the MyoD gene or the negative control primer pair Untr12. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K27me2, Monoclonal Antibody (Cat# AAA388895)
Full Name
Histone H3K27me2 antibody (mAb)(Clone MABI 0324)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, WB
Pricing
$600/0.1 mg | $2,645/5x0.1 mg
Western Blot (WB)
(Histone H4K20me2 antibody (mAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with the Histone H4K20me2 antibody (mAb) at 2 ug/ml.)
Histone H4K20me2, Monoclonal Antibody (Cat# AAA388939)
Full Name
Histone H4K20me2 antibody (mAb)(Clone MABI 0422)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
DB, WB
Purity
Protein G Chromatography
Pricing
$590/0.1 mg | $2,595/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 monomethyl Lys79 antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 monomethyl Lys79 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on ChIP DNA using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.)
Histone H3K79me1, Polyclonal Antibody (Cat# AAA388383)
Full Name
Histone H3K79me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, WB
Pricing
$200/0.01 mL | $600/0.2 mL | $2,645/5x0.2 mL
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys79 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ug of Histone H3 dimethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K79me2, Polyclonal Antibody (Cat# AAA388634)
Full Name
Histone H3K79me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, DB, WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3K27me1 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from a human B cell lymphoma cell line and 2 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Histone H3K27me1 binding sites. The image shows binding across a region of chromosome 6. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Histone H3K27me1, Monoclonal Antibody (Cat# AAA388723)
Full Name
Histone H3K27me1 antibody (mAb)(Clone MABI 0321)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, ICC, IF, WB
Purity
Protein G Chromatography
Pricing
$375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3K27me2 antibody (pAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from lung tumor chromatin and 6 ul Histone H3K27me2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.1 million sequence tags were mapped to identify Histone H3K27me2 binding sites.)
Histone H3K27me2, Polyclonal Antibody (Cat# AAA388427)
Full Name
Histone H3K27me2 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, ICC, IF, WB
Pricing
$200/0.01 mL | $600/0.2 mL | $2,645/5x0.2 mL
Chromatin Immunoprecipitation (ChIP)
(Histone H3K27me3 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from HeLa cells and 5 ul H3K27me3 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Histone H3K27me3, Polyclonal Antibody (Cat# AAA388388)
Full Name
Histone H3K27me3 antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, IP, WB
Pricing
$200/0.01 mL | $600/0.1 mL | $2,645/5x0.1 mL
Chromatin Immunoprecipitation (ChIP)
(hMeDIP-chip performed on human brain DNA using 5-Hydroxymethylcytosine (5-hmC) antibody. Human brain DNA (2 ug) was immunoprecipitated with 10 ug of 5-Hydroxymethylcytosine antibody. Following hMeDIP, the DNA was amplified, labeled and hybridized to an Affymetrix Human Tiling 2.0R Array. Shown is a region from chromosome 6q containing the ARID1B, ZDHHC14 and SNX9 genes. The results show that 5-hydroxymethylcytosine is enriched primarily in the coding regions of genes, rather than in the gene promoter or regulatory regions.)
5-Hydroxymethylcytosine, Monoclonal Antibody (Cat# AAA388665)
Full Name
5-Hydroxymethylcytosine (5-hmC) antibody (mAb)(Clone 59.1)
Reactivity
Human, Mouse
Applications
DB, ICC, IF, IHC, MeDIP
Purity
Protein G Chromatography
Pricing
$190/0.01 mg | $330/0.05 mg | $545/0.1 mg | $2,380/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3K36me3 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Histone H3K36me3 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Histone H3K36me3, Monoclonal Antibody (Cat# AAA388726)
Full Name
Histone H3K36me3 antibody (mAb)(Clone MABI 0333)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, IF, WB
Purity
Protein G Chromatography
Pricing
$375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys9 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of Histone H3 dimethyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Histone H3K9me2, Polyclonal Antibody (Cat# AAA388336)
Full Name
Histone H3K9me2 antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, DB, ICC, IF, WB
Pricing
$200/0.01 mL | $375/0.05 mL | $600/0.1 mL | $2,645/5x0.1 mL
ASXL1, Polyclonal Antibody (Cat# AAA9630778)
Full Name
ASXL1 Antibody
Gene Names
ASXL1; MDS; BOPS
Reactivity
Human, Mouse
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H4K20me3 Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from T-47D, mammary epithelial ductal cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H4K20me3 binding sites. The image shows binding across a region of chromosome 19.)
Histone H4K20me3, Antibody (Cat# AAA389440)
Full Name
AbFlex Histone H4K20me3 antibody (rAb)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, EIA, ICC, IF, WB
Purity
Ni-NTA
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H4K20me1 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 20 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify Histone H4K20me1 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Histone H4K20me1, Monoclonal Antibody (Cat# AAA388331)
Full Name
Histone H4K20me1 antibody (mAb)(Clone 5E10-D8)
Gene Names
HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
Reactivity
Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, DB, ICC, IF, WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Histone H3 dimethyl Lys4 antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit and chromatin from 500,000 primary mouse T cells. ChIP DNA was sequenced on the Illumina GA II and 25 million sequence tags were mapped to identify H3K4me2 binding sites. The image shows the expected H3K4me2 binding pattern with enrichment at promoters and at locations outside of promoters.)
Histone H3K4me2, Polyclonal Antibody (Cat# AAA388337)
Full Name
Histone H3K4me2 antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&Tag, DB, WB
Pricing
$200/0.01 mL | $375/0.05 mL | $600/0.1 mL | $2,645/5x0.1 mL
Chromatin Immunoprecipitation (ChIP)
(ChIP-Seq of Histone H3K9me3 pAb ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from D425 human Medulloblastoma cells and 10 ul of Histone H3K9me3 antibody (pAb). ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify H3K9me3 binding sites. H3K9me3 binds broadly across the genome. The image shows two different regions. The left box shows many binding sites across a broad region on chromosome 1. The right box is zoomed in to show a dispersed binding pattern across the RHOT1 gene.)
Histone H3K9me3, Polyclonal Antibody (Cat# AAA388348)
Full Name
Histone H3K9me3 antibody (pAb)
Reactivity
Fission Yeast, Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&Tag, DB, ICC, IF, IHC, WB
Pricing
$200/0.01 mL | $375/0.05 mL | $600/0.1 mL | $2,645/5x0.1 mL
Double-stranded RNA (dsRNA), Monoclonal ELISA Kit (Cat# AAA574026)
Full Name
Double-stranded RNA (dsRNA) ELISA kit (K1 based)
Reactivity
General
Applications
EIA
Pricing
$1,500/200 Tests | $6,975/5x500 Tests
Western Blot (WB)
(Histone H3 monomethyl Lys23 pAb tested by Western blot. HeLa cell nuclear extract (20 ug per lane) was probed with Histone H3 monomethyl Lys23 pAb at a dilution of 1:1,000.)
Histone H3K23me1, Polyclonal Antibody (Cat# AAA388469)
Full Name
Histone H3K23me1 antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Pricing
$200/0.01 mL | $590/0.2 mL | $2,595/5x0.2 mL
Wester Blot (WB)
(Dilution: WB: 1:1000-2000 IHC: 1:200-500)
Pan Methylated Lysine, Monoclonal Antibody (Cat# AAA9241643)
Full Name
Pan Methylated Lysine Monoclonal Antibody (Mix)
Reactivity
All Species
Applications
WB, IHC-P
Wester Blot (WB)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
MGMT, Polyclonal Antibody (Cat# AAA9244285)
Full Name
MGMT Polyclonal Antibody
Reactivity
Human, Rat
Applications
WB, IHC-P
DNMT3A dimethyl Lys44, Polyclonal Antibody (Cat# AAA388855)
Full Name
DNMT3A dimethyl Lys44 antibody (pAb)
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Pricing
$180/0.01 mL | $395/0.1 mL | $1,730/5x0.1 mL
Immunofluorescence (IF)
(Sample Data: Representative immunofluorescent stain with Anti-Methylated Lysine (Tri- epsilon-N-methyl), FITC Conjugated (1:1000) using the paraformaldehyde fixed human melanoma cells (MMRU).)
Methylated Lysine (Tri-epsilon-N-methyl), Polyclonal Antibody (Cat# AAA516057)
Full Name
Anti-Methylated Lysine (Tri-epsilon-N-methyl), FITC Conjugated
Reactivity
Pan-specific antibody
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
No cross-reactions detected with acetylated, mono-methylated, di-methylated proteins.
Applications
IF
Purity
By affinity chromatography on a tri-methyl lysine agarose column
Pricing
$435/0.1 mg | $1,735/5x0.1 mg
Dot Blot (DB)
(3-Methylcytosine (3-mC, 3-methylcytidine) antibody (pAb) tested by DNA dot blot BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000. Lane 1: 3-methylcytidine. Lane 2: Cytidine. Lane 3: 5-methylcytidine. Lane 4: 5-hydroxymethylcytodine.)
3-Methylcytosine, Polyclonal Antibody (Cat# AAA388795)
Full Name
3-Methylcytosine (3-mC) antibody (pAb)
Reactivity
Human, Mouse
Applications
DB, ICC, IF
Pricing
$190/0.01 mL | $545/0.1 mL | $2,380/5x0.1 mL
Western Blot (WB)
(Sample Data: Representative western blot of the mono-methylated arginine protein profile with Anti-Methylated Arginine (NG-Mono-methyl) (1:1000) using human melanoma (MMRU) cell lysate (A) and the MMRU lysate plus additional mono-methyl arginine peptide inhibitor (B).)
Methylated Arginine (NG-Mono-methyl), Polyclonal Antibody (Cat# AAA515854)
Full Name
Anti-Methylated Arginine (NG-Mono-methyl)
Reactivity
Pan-specific antibody
No reaction to acetylated proteins, di-methylated, tri-methylated proteins.
No reaction to acetylated proteins, di-methylated, tri-methylated proteins.
Applications
WB, EIA, IHC, IF
Purity
By affinity chromatography on a mono-methyl arginine agarose column
Pricing
$435/0.1 mg | $1,735/5x0.1 mg
