Chromatin Immunoprecipitation (ChIP)
(Histone H3R8me2a antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a mouse osteoblast cell line and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Rabbit Histone H3R8me2a Polyclonal Antibody | anti-H3R8me2a antibody
Histone H3R8me2a (asymmetric) antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Purity
Affinity Purified
Synonyms
Histone H3R8me2a; Polyclonal Antibody; Histone H3R8me2a (asymmetric) antibody (pAb); anti-H3R8me2a antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3R8me2a antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ul per ChIP
ChIP-Seq: 10ul each
WB: 1:500 dilution ChIP-Seq validation was performed's Epigenetics Services.
ChIP: 10ul per ChIP
ChIP-Seq: 10ul each
WB: 1:500 dilution ChIP-Seq validation was performed's Epigenetics Services.
Conjugation
Methylated
Immunogen
This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.
Abs Residue
Arg
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3R8me2a antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a mouse osteoblast cell line and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3R8me2a antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a mouse osteoblast cell line and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Western Blot (WB)
(Histone H3 dimethyl Arg8 asymmetric pAb tested by Western blot. Nuclear extract from U2OS cells (20 ug) or HEK293 cells (20 ug) was probed with Histone H3 dimethyl Arg8 asymmetric pAb (1:500 dilution).)
Western Blot (WB)
(Histone H3 dimethyl Arg8 asymmetric pAb tested by Western blot. Nuclear extract from U2OS cells (20 ug) or HEK293 cells (20 ug) was probed with Histone H3 dimethyl Arg8 asymmetric pAb (1:500 dilution).)
Dot Blot (DB)
(Histone H3 dimethyl Arg8 asymmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg8 asymmetric pAb for dimethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg8 asymmetric pAb at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmod Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmod Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym). Bottom Panel: Lane 1: unmod Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (sym). Lane 4: dimethyl-Arg17 H3 (asym). Lane 5: unmod Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (sym). Lane 8: dimethyl-Arg26 H3 (asymmetric). No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to Lys4, Lys9, Lys18, Lys23 and Lys27 of histone H3 was observed.)
Dot Blot (DB)
(Histone H3 dimethyl Arg8 asymmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg8 asymmetric pAb for dimethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg8 asymmetric pAb at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmod Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmod Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym). Bottom Panel: Lane 1: unmod Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (sym). Lane 4: dimethyl-Arg17 H3 (asym). Lane 5: unmod Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (sym). Lane 8: dimethyl-Arg26 H3 (asymmetric). No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to Lys4, Lys9, Lys18, Lys23 and Lys27 of histone H3 was observed.)
Related Product Information for anti-H3R8me2a antibody
Short Description: Our Histone H3R8me2a (asymmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
Product Categories/Family for anti-H3R8me2a antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3R8me2a (Catalog #AAA388527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3R8me2a (asymmetric) antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3R8me2a can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP ChIP-Seq: 10ul each WB: 1:500 dilution ChIP-Seq validation was performed's Epigenetics Services. Researchers should empirically determine the suitability of the H3R8me2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3R8me2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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