Dot Blot (DB)
(5-mCpA Antibody Specificity Dot blot analysis was used to confirm the specificity of 5-mCpA antibody for the 5-mCpA dinucleotide. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with 5-mCpA (1 ug/ml dilution). Column 1: 5-meCpA. Column 2: CpA. Column 3: 5-meCpC. Column 4: 5-meCpT. Column 5: 5-meCpG. Column 6: Ap5mC)
Mouse 5-mCpA Monoclonal Antibody | anti-5-mCpA antibody
5-mCpA antibody (mAb)(Clone 2C8H8A6)
Reactivity
Not Species Specific
Applications
Dot Blot
Purity
Protein A Chromatography
Synonyms
5-mCpA; Monoclonal Antibody; 5-mCpA antibody (mAb)(Clone 2C8H8A6); Methyl-CpA; 5-methyl-cytosine-adenosine di-nucleoside; 2C8H8A6; anti-5-mCpA antibody
Host
Mouse
Reactivity
Not Species Specific
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2C8H8A6
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-5-mCpA antibody
Dot Blot (DB)
Application Notes
Applications Validated:
DB: 1ug/ml
DB: 1ug/ml
Conjugation
Methylated
Immunogen
This antibody was raised against 5-methyl-cytosine-adenosine di-nucleoside conjugated to KLH and recognized 5-mCpA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Dot Blot (DB)
(5-mCpA Antibody Specificity Dot blot analysis was used to confirm the specificity of 5-mCpA antibody for the 5-mCpA dinucleotide. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with 5-mCpA (1 ug/ml dilution). Column 1: 5-meCpA. Column 2: CpA. Column 3: 5-meCpC. Column 4: 5-meCpT. Column 5: 5-meCpG. Column 6: Ap5mC)
Dot Blot (DB)
(5-mCpA Antibody Specificity Dot blot analysis was used to confirm the specificity of 5-mCpA antibody for the 5-mCpA dinucleotide. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with 5-mCpA (1 ug/ml dilution). Column 1: 5-meCpA. Column 2: CpA. Column 3: 5-meCpC. Column 4: 5-meCpT. Column 5: 5-meCpG. Column 6: Ap5mC)
Related Product Information for anti-5-mCpA antibody
Short Description: Our 5-mCpA antibody (mAb)(Clone 2C8H8A6) was raised in a Mouse host. It has been validated for use in Dot blot; the sequence is not species specific so it should react with a wide range of sample types.
Background: DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or "suppression" with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression. DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or "suppression" with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression.
Background: DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or "suppression" with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression. DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or "suppression" with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression.
Product Categories/Family for anti-5-mCpA antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The 5-mCpA (Catalog #AAA389043) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 5-mCpA antibody (mAb)(Clone 2C8H8A6) reacts with Not Species Specific and may cross-react with other species as described in the data sheet. AAA Biotech's 5-mCpA can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB). Applications Validated: DB: 1ug/ml. Researchers should empirically determine the suitability of the 5-mCpA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-mCpA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
