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266 results for "autophagic-related-protein" - showing 50-100
Autophagy-related protein 8 (atg8), Recombinant Protein (Cat# AAA1191307)
Full Name
Recombinant Neosartorya fischeri Autophagy-related protein 8 (atg8)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,175/1 mg (E-Coli) | $1,735/1 mg (Yeast) | $785/0.2 mg (E-Coli) | $1,050/0.2 mg (Yeast) | $605/0.05 mg (E-Coli) | $780/0.05 mg (Yeast) | $2,210/1 mg (Baculovirus) | $1,170/0.1 mg (Baculovirus) | $1,820/0.1 mg (Mammalian-Cell) | $860/0.5 mg (E-Coli) | $1,150/0.5 mg (Yeast) | $955/0.05 mg (Baculovirus) | $1,195/0.05 mg (Mammalian-Cell) | $1,695/0.5 mg (Baculovirus)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6001189)
Full Name
MAP1ALC3, NT (LC3, APG8A, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associa
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
EL/EIA, WB, IHC, IF
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6003770)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IHC, IF
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with MBS9211609 LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9211609 LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
LC3, Polyclonal Antibody (Cat# AAA9211609)
Full Name
LC3 Antibody (APG8A)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
IF, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AP1800a LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1800a LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6009284)
Full Name
MAP1LC3A (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6002582)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC, IF
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Autophagy-related protein 8 (atg8), Recombinant Protein (Cat# AAA1366054)
Full Name
Recombinant Neosartorya fumigata Autophagy-related protein 8 (atg8)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,175/1 mg (E-Coli) | $1,735/1 mg (Yeast) | $785/0.2 mg (E-Coli) | $1,050/0.2 mg (Yeast) | $605/0.05 mg (E-Coli) | $780/0.05 mg (Yeast) | $2,210/1 mg (Baculovirus) | $1,170/0.1 mg (Baculovirus) | $1,820/0.1 mg (Mammalian-Cell) | $860/0.5 mg (E-Coli) | $1,150/0.5 mg (Yeast) | $955/0.05 mg (Baculovirus) | $1,195/0.05 mg (Mammalian-Cell) | $1,695/0.5 mg (Baculovirus)
LC3 (APG8c), Blocking Peptide (Cat# AAA9223070)
Full Name
LC3 Antibody (APG8c) (N-term) Blocking Peptide
Gene Names
MAP1LC3C; LC3C; ATG8J
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801d LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6001733)
Full Name
MAP1LC3A (D106) (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated pro
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
LC3 (APG8a/b), Blocking Peptide (Cat# AAA9223063)
Full Name
LC3 Antibody (APG8a/b) Blocking Peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9209718 Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Polyclonal Antibody (Cat# AAA9209718)
Full Name
Beclin 1 Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat, Xenopus)
Applications
IF, EIA, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
LC3 (APG8A), Blocking Peptide (Cat# AAA9223036)
Full Name
LC3 Antibody (APG8A) Blocking peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Bi-APG8b (MAP1LC3B) (T93/Y99), Blocking Peptide (Cat# AAA9225247)
Full Name
Bi-Phospho-APG8b(MAP1LC3B)(T93/Y99) Antibody Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
LC3 (APG8C) (T48), Blocking Peptide (Cat# AAA9223071)
Full Name
LC3 Antibody (APG8C) (T48) Blocking peptide
Gene Names
MAP1LC3C; LC3C; ATG8J
LC3 (APG8B) (T6), Blocking Peptide (Cat# AAA9223057)
Full Name
LC3 Antibody (APG8B) (T6) Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
LC3C (S12), Blocking Peptide (Cat# AAA9225089)
Full Name
Phospho-LC3C(S12) Antibody Blocking peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
LC3 (APG8a), Blocking Peptide (Cat# AAA9223045)
Full Name
LC3 Antibody (APG8a) (N-term) Blocking Peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
LC3 (APG8B), Blocking Peptide (Cat# AAA9223056)
Full Name
LC3 Antibody (APG8B) (C-term) Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Cleaved LC3B, Blocking Peptide (Cat# AAA9223091)
Full Name
Cleaved LC3B Antibody Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
LC3 (APG8a) (D106), Blocking Peptide (Cat# AAA9223047)
Full Name
LC3 Antibody (APG8a) (D106) Blocking Peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
MAP1LC3A (S12), Blocking Peptide (Cat# AAA9230816)
Full Name
Phospho-MAP1LC3A(S12) Blocking Peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
LC3B (T12), Blocking Peptide (Cat# AAA9225173)
Full Name
Phospho-LC3B(T12) Antibody Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
LC3 (APG8B), Blocking Peptide (Cat# AAA9223055)
Full Name
LC3 Antibody (APG8B) (N-term) Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Cleaved LC3A, Blocking Peptide (Cat# AAA9223080)
Full Name
Cleaved LC3A Antibody Blocking peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Western Blot (WB)
(Western blot analysis of LC3B expression in C6 cell lysate (MBS179682).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP1LC3B )
LC3B, Monoclonal Antibody (Cat# AAA179682)
Full Name
Anti-LC3B Rabbit Monoclonal Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
LC3 (APG8A) (P45), Blocking Peptide (Cat# AAA9223046)
Full Name
LC3 Antibody (APG8A) (P45) Blocking peptide
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Western Blot (WB)
(Western blot analysis of MAP1LC3A expression in Human brain lysate (MBS179686).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP1LC3A )
MAP1LC3A/Lc3A, Monoclonal Antibody (Cat# AAA179686)
Full Name
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
MAP1LC3A, Blocking Peptide (Cat# AAA9229299)
Full Name
MAP1LC3A Blocking Peptide (C-term)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody(MBS179687)MAP1LC3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (MBS179687)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MAP1LC3A/Lc3A, Monoclonal Antibody (Cat# AAA179687)
Full Name
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extract from K-562, Hela, JK and 293 cells using LC3A Antibody)
LC3A, Polyclonal Antibody (Cat# AAA9613053)
Full Name
LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB)
(Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)
ATG12, Polyclonal Antibody (Cat# AAA6463468)
Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (APC)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AP1800a LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1800a LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318323)
Full Name
MAP1LC3A (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318293)
Full Name
MAP1ALC3, NT (LC3, APG8A, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associa
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Rabbit
Applications
IF, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of LC3B Antibody expression in Rat liver tissue lysates.)
LC3B, Polyclonal Antibody (Cat# AAA9613358)
Full Name
LC3B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276023)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (MaxLight 405)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Western Blot (WB)
(Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)
ATG12, Polyclonal Antibody (Cat# AAA6463467)
Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (AP)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Monoclonal Antibody (Cat# AAA6120970)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (APC)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$780/0.1 mL | $3,505/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801d LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318318)
Full Name
MAP1LC3A (D106) (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated pro
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
WB, ICC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Monoclonal Antibody (Cat# AAA6121638)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (PE)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$780/0.1 mL | $3,505/5x0.1 mL
Western Blot (WB)
(Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)
ATG12, Polyclonal Antibody (Cat# AAA6463470)
Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (FITC)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Monoclonal Antibody (Cat# AAA6121318)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (FITC)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$780/0.1 mL | $3,505/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275953)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (APC)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275954)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (Biotin)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Monoclonal Antibody (Cat# AAA6121464)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (Azide free) (HRP)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$780/0.1 mL | $3,505/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP10648a APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAPG8a/b, Polyclonal Antibody (Cat# AAA6318737)
Full Name
MAPG8a/b (MAP1LC3A/B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-a
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318255)
Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EIA, IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276204)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (PE)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP10648a APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAPG8a/b, Polyclonal Antibody (Cat# AAA6318739)
Full Name
MAPG8a/b (MAP1LC3A/B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-a
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Testing Data
(IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)
MAP1LC3A, Monoclonal Antibody (Cat# AAA6121027)
Full Name
MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 l
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$910/0.2 mL | $4,085/5x0.2 mL
Testing Data
(IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)
MAP1LC3A, Monoclonal Antibody (Cat# AAA6121201)
Full Name
MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 l
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$910/0.2 mL | $4,085/5x0.2 mL
