Rabbit anti-Human ATG12 Polyclonal Antibody | anti-ATG12 antibody

ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (APC)

Cat. Num. AAA6275953

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG12; Polyclonal Antibody; NT (ATG12; APG12; APG12L; Ubiquitin-like protein ATG12; Autophagy-related protein 12) (APC); Autophagy-related protein 12; anti-ATG12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ATG12 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100; Applications are based on unconjugated antibody.
• Immunogen: ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44~73 amino acids from the N-term of human APG12L.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Western Blot (WB)

(Western blot analysis of anti-APG12L Pab in Hela cell lysate. APG12L (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ATG12 antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.

Product Categories/Family for anti-ATG12 antibody

Antibodies; Autophagy

References

Yee, K.S. et al. Cell Death Differ. August; 16(8): 1135?145.(2009). Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 119569340
• NCBI GeneID: 9140
• UniProt Accession #: O94817; Q6PJV2
• Molecular Weight: 7,746 Da
• NCBI Official Full Name: ATG12 autophagy related 12 homolog (S. cerevisiae), isoform CRA_b
• NCBI Official Synonym Full Names: autophagy related 12
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L; APG12-like
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation