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266 results for "autophagic-related-protein" - showing 200-250
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318346)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318257)
Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EIA, IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318347)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318349)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276194)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (AP)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276203)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (MaxLight 750)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813c ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276224)
Full Name
ATG7 (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (MaxLight 650)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276021)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (FITC)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276024)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (MaxLight 490)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276027)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (MaxLight 750)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276028)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (PE)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276020)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (Biotin)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Beclin 1, Polyclonal Antibody (Cat# AAA9613615)
Full Name
Beclin 1 Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Applications
EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)
ATG12, Polyclonal Antibody (Cat# AAA6463477)
Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (PE)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
IF, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP13795a Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Polyclonal Antibody (Cat# AAA6278112)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (FITC)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8104b APG1 (ULK1) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ULK1, Polyclonal Antibody (Cat# AAA6504752)
Full Name
ULK1, CT (Serine/Threonine-protein Kinase ULK1, Unc-51-like Kinase 1, ATG1, KIAA0722) (FITC)
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human
Applications
WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with AP1800a LC3 (APG8A) antibody.U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1800a LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318329)
Full Name
MAP1LC3A (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275957)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (MaxLight 405)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801d LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318309)
Full Name
MAP1LC3A (D106) (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated pro
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
WB, ICC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with APG5 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c APG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
APG5, Polyclonal Antibody (Cat# AAA6274268)
Full Name
APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (PE)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human
Applications
IHC, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG4D (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP11448a ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG4D, Polyclonal Antibody (Cat# AAA6276176)
Full Name
ATG4D, NT (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D) (HRP)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Mouse
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG4D (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP11448a ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG4D, Polyclonal Antibody (Cat# AAA6276181)
Full Name
ATG4D, NT (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D) (MaxLight 750)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276228)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (APC)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1805a cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318333)
Full Name
MAP1LC3A, cleaved (LC3B, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse
Applications
IHC, WB, ICC
Purity
Purified by Peptide Immunoaffinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276232)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (MaxLight 405)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276236)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (MaxLight 750)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276237)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (PE)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1805a cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318332)
Full Name
MAP1LC3A, cleaved (LC3B, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse
Applications
IHC, EIA, WB, ICC
Purity
Purified by Peptide Immunoaffinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813c ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276218)
Full Name
ATG7 (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (Biotin)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318351)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276195)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (APC)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276200)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (MaxLight 490)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318260)
Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
IF, ICC
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318344)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG5, Polyclonal Antibody (Cat# AAA6276198)
Full Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (HRP)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817b ATG16L primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG16L, Polyclonal Antibody (Cat# AAA6276026)
Full Name
ATG16L (ATG16L1, APG16L, Autophagy-related protein 16-1, APG16-like 1) (MaxLight 650)
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318286)
Full Name
MAP1ALC3, NT (LC3, APG8A, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associa
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Rabbit
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1ALC3, Polyclonal Antibody (Cat# AAA6318288)
Full Name
MAP1ALC3, NT (LC3, APG8A, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associa
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Rabbit
Applications
IF, EIA, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with Beclin 1 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP13795a Beclin 1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Beclin 1, Polyclonal Antibody (Cat# AAA6278119)
Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (PE)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801d LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318308)
Full Name
MAP1LC3A (D106) (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated pro
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
WB, EIA, ICC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801d LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318312)
Full Name
MAP1LC3A (D106) (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated pro
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
WB, EIA, ICC
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275956)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (HRP)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275959)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (MaxLight 550)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275958)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (MaxLight 490)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG12, Polyclonal Antibody (Cat# AAA6275961)
Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12) (MaxLight 750)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.1 mL | $3,990/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276231)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (HRP)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1805a cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318334)
Full Name
MAP1LC3A, cleaved (LC3B, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse
Applications
IHC, EIA, WB, ICC
Purity
Purified by Peptide Immunoaffinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data.)
ATG7, Polyclonal Antibody (Cat# AAA6276230)
Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein) (FITC)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1814c ATG9A (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
ATG9A, Polyclonal Antibody (Cat# AAA6276273)
Full Name
ATG9A, CT (ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9) (Biotin)
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)
MAP1LC3A, Polyclonal Antibody (Cat# AAA6318342)
Full Name
MAP1LC3A, NT (LC3, APG8A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protei
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA, WB, IF
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
