LC3 (APG8c) Blocking Peptide | MAP1LC3C blocking peptide

LC3 Antibody (APG8c) (N-term) Blocking Peptide

• Gene Names: MAP1LC3C; LC3C; ATG8J
• Synonyms: LC3 (APG8c); LC3 Antibody (APG8c) (N-term) Blocking Peptide; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma; MAP1LC3C; MAP1LC3C blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-terminal region of human LC3 (APG8c). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 147

• Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes
• Tissue Location: Most abundant in placenta, lung and ovary.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAP1LC3C blocking peptide

Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruites all ATG8 family members to infecting bacteria such as S.Typhimurium.

NCBI and Uniprot Product Information

• NCBI GI #: 84028113
• NCBI GeneID: 440738
• UniProt Accession #: Q9BXW4; A0PJY8; A2RUP0
• Molecular Weight: 16,852 Da
• NCBI Official Full Name: Microtubule-associated proteins 1A/1B light chain 3C
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 gamma
• NCBI Official Symbol: MAP1LC3C
• NCBI Official Synonym Symbols: LC3C; ATG8J
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3C
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3C
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3C
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 C
• UniProt Entry Name: MLP3C_HUMAN

NCBI Description

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]

Uniprot Description

LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes.

Protein type: Autophagy; Ubiquitin-like modifier; Vesicle; Microtubule-binding

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: autophagic vacuole; cytosol

Molecular Function: microtubule binding; protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation