LC3 (APG8a) Blocking Peptide | MAP1LC3A blocking peptide

LC3 Antibody (APG8a) (N-term) Blocking Peptide

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Synonyms: LC3 (APG8a); LC3 Antibody (APG8a) (N-term) Blocking Peptide; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1LC3A; MAP1LC3A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-terminal region of human LC3 (APG8a). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 121

• Cellular Location: Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes
• Tissue Location: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAP1LC3A blocking peptide

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

NCBI and Uniprot Product Information

• NCBI GI #: 85701362
• NCBI GeneID: 84557
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14,493 Da
• NCBI Official Full Name: Microtubule-associated proteins 1A/1B light chain 3A
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Ubiquitin-like modifier; Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: autophagic vacuole; cytosol

Molecular Function: microtubule binding; phosphatidylethanolamine binding; phospholipid binding; protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation