Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " IgG Fc" - showing 850-900
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody 5D12 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 5D12 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD40, Monoclonal Recombinant Antibody (Cat# AAA488354)
Full Name
Anti-CD40 [5D12]
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Cynomolgus, Marmoset Monkey, Human
Applications
BL, EIA, FC/FACS, SPR, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot analysis of HLA-DRA Antibody (N-term) in Ramos cell line lysates (35ug/lane). HLA-DRA (arrow) was detected using the purified Pab.)
HLA Class 2 Antigen DRA, Polyclonal Antibody (Cat# AAA629152)
Full Name
HLA Class 2 Antigen DRA, NT (HLA Class II Histocompatibility Antigen DR alpha Chain, HLA-DRA, HLA-DRA1, FLJ51114, MHC Class II Antigen DRA, MLRW)
Gene Names
HLA-DRA; MLRW; HLA-DRA1
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Purified by ammonium sulfate precipitation.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Western Blot (WB)
ATP synthase subunit gamma, Monoclonal Antibody (Cat# AAA435251)
Full Name
ATP synthase subunit gamma Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 [4C7] antibody at 10 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 10 μg/mL.)
PD-1, Monoclonal Antibody (Cat# AAA154533)
Full Name
PD-1 Antibody [4C7]
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF, FC/FACS
Purity
Protein A purified IgG1.
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Western Blot (WB)
(Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μg/ml)
LIGHT, Monoclonal Antibody (Cat# AAA154599)
Full Name
LIGHT Antibody [7B9E12]
Gene Names
TNFSF14; LTg; CD258; HVEML; LIGHT
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF, FC/FACS
Purity
Protein A purified
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))
CD3, Monoclonal Antibody (Cat# AAA210022)
Full Name
MOUSE ANTI HUMAN CD3:APC
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Parathyroid stained with Chromogranin A Rabbit Polyclonal Antibody)
Chromogranin A/CHGA, Polyclonal Antibody (Cat# AAA439873)
Full Name
Chromogranin A/CHGA (Neuroendocrine Marker)
Gene Names
CHGA; CGA
Reactivity
Human.
Does not react with rat. Others not known.
Does not react with rat. Others not known.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fas antibody R-125224 (Ab00802) Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (Ab00802-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fas, Monoclonal Recombinant Antibody (Cat# AAA488362)
Full Name
Anti-Fas [R-125224]
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216585)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Low Endotoxin
Applications
FC/FACS, IF, WB
Pricing
$830/0.5 mg | $3,580/5x0.5 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Rat anti Human CD195:RPE (MBS213738))
CD195, Monoclonal Antibody (Cat# AAA212544)
Full Name
RAT ANTI HUMAN CD195
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Applications
FC/FACS
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Staining of HeLa cells with Mouse anti Human CD49a (MBS210309))
CD49a, Monoclonal Antibody (Cat# AAA210620)
Full Name
MOUSE ANTI HUMAN CD49a:FITC
Gene Names
ITGA1; VLA1; CD49a
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody. )
TFAP2A, Monoclonal Antibody (Cat# AAA120100)
Full Name
Mouse monoclonal antibody Anti-Human TFAP2A
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human
Applications
DB, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(NANOG Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).)
NANOG, Polyclonal Antibody (Cat# AAA9212234)
Full Name
NANOG Antibody (Center)
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Applications
IF, EIA, WB, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Erythropoietin (EPO), Monoclonal Antibody (Cat# AAA439935)
Full Name
Erythropoietin (EPO)
Gene Names
EPO; EP; DBAL; ECYT5; MVCD2
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20 degree C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.)
HSP60, Monoclonal Antibody (Cat# AAA802596)
Full Name
Hsp60 Antibody: FITC
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Soybean
Applications
WB, IP, EIA, FC/FACS, IHC
Pricing
$435/0.2 mg | $1,735/5x0.2 mg
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD14-IgG:Endotoxin Low (MBS224504))
CD14, Monoclonal Antibody (Cat# AAA213102)
Full Name
MOUSE ANTI HUMAN CD14
Applications
FC/FACS, IF, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)
CD4, Monoclonal Antibody (Cat# AAA210256)
Full Name
MOUSE ANTI RAT CD4 (DOMAIN 1):Preservative Free
Gene Names
Cd4; p55; W3/25
Applications
FC/FACS, FN
Pricing
$765/1 mg | $3,290/5x1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Cervical Squamous Cell Carcinoma stained with CD44v4 Rabbit Recombinant Monoclonal Antibody (CD44v4/1700R).)
CD44v4, Monoclonal Antibody (Cat# AAA4380359)
Full Name
CD44v4 (Marker of Tumor Metastasis)
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human. Others not tested.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1. Western blot analysis of PNP using anti-PNP antibody (MBS178628).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: rat ovary tissue lysates,Lane 3: mouse liver tissue lysates,Lane 4: human placneta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PNP at approximately 38KD. The expected band size for PNP is at 38KD. )
PNP, Polyclonal Antibody (Cat# AAA178628)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215797)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$375/0.1 mg | $1,535/5x0.1 mg
Testing Data
(Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 ug/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.)
OX-62, Monoclonal Antibody (Cat# AAA212002)
Full Name
MOUSE ANTI RAT OX-62
Applications
FC/FACS, IP, WB
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Immunohistochemistry (IHC)
(Anti-VTCN1 / B7-H4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
VTCN1 / B7-H4, Monoclonal Antibody (Cat# AAA246437)
Full Name
Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Human
Applications
IHC - Paraffin, FC/FACS
Purity
Protien G purified from tissue culture supernatant
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using CAPN1 Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200385 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
CAPN1, Monoclonal Antibody (Cat# AAA9200385)
Full Name
CAPN1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Notch 1 antibody E6 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Notch 1, Recombinant Antibody (Cat# AAA488228)
Full Name
Anti-Notch 1 [E6]
Reactivity
Human, Mouse
Applications
IHC
Purity
Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC (MBS211114))
CD45, Monoclonal Antibody (Cat# AAA210029)
Full Name
MOUSE ANTI HUMAN CD45:APC
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). MBS9212584 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Pbk, Polyclonal Antibody (Cat# AAA9212584)
Full Name
Mouse Pbk Antibody (N-term)
Gene Names
Pbk; TOPK; AW538537; D14Ertd732e; 2810434B10Rik
Reactivity
Human, Mouse
Applications
EIA, IHC-P, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD18 (Activation Epitope):Biotin (MBS210392))
CD18, Monoclonal Antibody (Cat# AAA212479)
Full Name
MOUSE ANTI HUMAN CD18 (ACTIVATION EPITOPE)
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, IP, WB
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Testing Data
(Formalin-paraffin non-Hodgkin's lymphoma stained with Lambda Light Chain Ab (ICO106). Note cell membrane and cytoplasmic staining.)
Lambda Light Chain, Monoclonal Antibody (Cat# AAA438460)
Full Name
Lambda Light Chain
Reactivity
Human.
Applications
EIA, FC/FACS, IF, WB, IP, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
(Overlay histogram showing ZF4 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ak2, Polyclonal Antibody (Cat# AAA9232174)
Full Name
Zebrafish ak2 Antibody (N-term)
Gene Names
ak2; wu:fb34f05; wu:fj80e03
Reactivity
Zebrafish
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
ELISA (EIA)
( Binding curve of anti-B7-H3 antibody MJ18 to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein (Pr00158-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
B7-H3, Monoclonal Recombinant Antibody (Cat# AAA488371)
Full Name
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
ELISA (EIA)
( Binding curve of anti-ICOSL antibody HK5.3 to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein (Pr00157-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
ICOSL, Monoclonal Recombinant Antibody (Cat# AAA488367)
Full Name
Anti-ICOSL [HK5.3]
Gene Names
Icosl; B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808698)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Testing Data
(Published customer image:Representative pictures of immunohistochemical detection of CD163+ cells in tracheobronchial lymph node and spleen. CD163+ cells were detected in tracheobronchial lymph nodes from control (A) and APP-infected pigs (B) and in spleen from control (C) and APP-infected pigs (D). Immunohistochemical visualization: horseradish peroxidase, brown substrate, hematoxylin counterstain; c, cortex; ca, central artery; e, ellipsoid; f, follicle; mz, marginal zone; pals, periarterial lymphatic sheath, rp, red pulp; s, subcapsular sinus.From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines. Vet Res. 2013 Oct 17;44:98.)
CD163, Monoclonal Antibody (Cat# AAA217155)
Full Name
MOUSE ANTI PIG CD163:RPE
Applications
FC/FACS
Pricing
$695/100 Tests | $2,975/5x100 Tests
Western Blot (WB)
Puma, Monoclonal Antibody (Cat# AAA435293)
Full Name
Puma mAb
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Pricing
$405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 (MBS212549), green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
CD13, Monoclonal Antibody (Cat# AAA213739)
Full Name
RAT ANTI MOUSE CD13:RPE
Gene Names
Anpep; Apn; AP-M; AP-N; Cd13; P150
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Testing Data
(Western blot analysis of extracts of mouse spleen cell lines, using FCER1A antibody.)
FCER1A, Antibody (Cat# AAA8501490)
Full Name
FCER1A Antibody
Gene Names
FCER1A; FCE1A; FcERI
Reactivity
Human
Applications
WB, IHC
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
Caspase-2, Monoclonal Antibody (Cat# AAA435300)
Full Name
Caspase-2 mAb
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Pricing
$405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
NDUFS1, Monoclonal Antibody (Cat# AAA435268)
Full Name
NDUFS1 Mouse mAb
Gene Names
NDUFS1; CI-75k; CI-75Kd; PRO1304
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IKBKB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
IKKbeta, Polyclonal Antibody (Cat# AAA125216)
Full Name
IKKbeta
Gene Names
IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data #1
(Human peripheral blood monocytes stained with Mouse anti Human Siglec-9 (MBS224422) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
SIGLEC-9, Monoclonal Antibody (Cat# AAA224537)
Full Name
MOUSE ANTI HUMAN SIGLEC-9:RPE
Gene Names
SIGLEC9; CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunocytochemistry (IHC)
(Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
CD80, Monoclonal Antibody (Cat# AAA154580)
Full Name
CD80 Antibody [7A2]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
EIA, IHC, ICC, IF, FC/FACS
Purity
Protein A purified
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Monoclonal Antibody (C6/372+3F7B5).)
CD6, Monoclonal Antibody (Cat# AAA438051)
Full Name
CD6 Mouse Monoclonal Antibody
Gene Names
CD6; TP120
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(MBS9607660 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
FCGR3A, Polyclonal Antibody (Cat# AAA9607660)
Full Name
FCGR3A Antibody
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD8a antibody YTS 105.18 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of YTS 105.18 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD8 alpha, Recombinant Antibody (Cat# AAA488138)
Full Name
Anti-CD8 alpha [YTS 105.18]
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS, IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR antibody Matuzumab HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
EGFR, Recombinant Antibody (Cat# AAA488209)
Full Name
Anti-EGFR [Matuzumab]
Reactivity
Human
Applications
FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD55 (Complement decay-accelerating factor) antibody LU-30 U93 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of LU-30 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
CD55, Monoclonal Recombinant Antibody (Cat# AAA488248)
Full Name
Anti-CD55 [LU30]
Gene Names
CD55; CR; TC; DAF; CROM; CHAPLE
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of human peripheral blood monocytes with Mouse anti Human CD49f:RPE (MBS213609))
CD49f, Monoclonal Antibody (Cat# AAA215048)
Full Name
MOUSE ANTI HUMAN CD49f
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Applications
FC/FACS, WB
Pricing
$195/0.025 mg | $730/5x0.025 mg
Immunohistochemistry (IHC)
(Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/1783R).)
CD45RB, Monoclonal Antibody (Cat# AAA4380196)
Full Name
CD45RB (B-Cell Marker)
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Ovarian Carcinoma stained with EpCAM Monoclonal Antibody (EGP40/1384).)
Ep-CAM/CD326, Monoclonal Antibody (Cat# AAA4380090)
Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human. Others not known.
Applications
EIA, FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
CD11c, Monoclonal Antibody (Cat# AAA438763)
Full Name
CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody
Gene Names
ITGAX; CD11C; SLEB6
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
