Rabbit Chromogranin A/CHGA Polyclonal Antibody | anti-CHGA antibody

Chromogranin A/CHGA (Neuroendocrine Marker)

Cat. Num. AAA439873

• Gene Names: CHGA; CGA
• Reactivity: Human.; Does not react with rat. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Chromogranin A/CHGA; Polyclonal Antibody; Chromogranin A/CHGA (Neuroendocrine Marker); Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; Vasostatin I or II; anti-CHGA antibody

• Host: Rabbit
• Reactivity: Human.; Does not react with rat. Others not known.
• Clonality: Polyclonal
• Isotype: IgG, kappa
• Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
• Form/Format: 200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 457

• Applicable Applications for anti-CHGA antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; IHC-F: 0.25-0.5ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human full-length Chromogranin A protein
• Cellular Localization: Finely granular Cytoplasmic
• Hu-chromosome Location: 14q32.12
• Positive Control: PC12 cells. Adrenal gland, bowel, parathyroid, pancreas, or pheochromocytoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Parathyroid stained with Chromogranin A Rabbit Polyclonal Antibody)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Pancreas stained with Chromogranin A Rabbit Polyclonal Antibody)

References

Konecki DS et. al. J Biol Chem 1987; 262:17026-30. Lloyd RV et. al. Am J Pathol 1988; 130:296-304.

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266.1
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 68-75kDa
• NCBI Official Full Name: chromogranin-A isoform 1 preproprotein
• NCBI Official Synonym Full Names: chromogranin A
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A
• UniProt Protein Name: Chromogranin-A
• Protein Family: Chromogranin
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).