Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Notch 1 antibody E6 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Notch 1 Recombinant Antibody | Notch1 recombinant antibody
Anti-Notch 1 [E6]
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
Notch 1; Anti-Notch 1 [E6]; Mis6; N1; Tan1; lin-12; NOTCH1; TAN1; hN1; Notch1 recombinant antibody
Reactivity
Human, Mouse
Isotype
Rabbit IgG, Lambda
Clone Number
E6
Specificity
An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1 mg/ml (varies by lot)
Applicable Applications for Notch1 recombinant antibody
ELISA, Immunohistochemistry (IHC)
Immunogen
This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Notch 1 antibody E6 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Notch 1 antibody E6 Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of E6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of E6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for Notch1 recombinant antibody
Recombinant monoclonal antibody to Notch 1. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the phage display derived scfv E6.
Product Categories/Family for Notch1 recombinant antibody
References
Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J.Methods. 2012 Sep;58(1):69-78. PMID:22842086
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
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Product Notes
The Notch1 (Catalog #AAA488228) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Notch 1 [E6] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Notch 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Notch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch 1, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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