Typical Testing Data/Standard Curve (for reference only)
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Canine N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) CLIA Kit | NT-ProBNP clia kit
Canine N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) CLIA Kit
Gene Names
NPPB; BNP
Reactivity
Canine
Applications
Chemiluminescence Immunoassay (CLIA)
Synonyms
N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP); Canine N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) CLIA Kit; N-Terminal Pro-Brain Natriuretic Peptide; NT-Pro-BNP; ; N-BNP; NT-ProBNP clia kit
Reactivity
Canine
Specificity
This assay has high sensitivity and excellent specificity for detection of NT-ProBNP. No significant cross-reactivity or interference between NT-ProBNP and analogues was observed.
Sequence Length
140
Applicable Applications for NT-ProBNP clia kit
Chemiluminescence Immunoassay (CLIA)
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
19.5-5,000pg/mL
Sensitivity
< 6.9pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NT-ProBNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NT-ProBNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for NT-ProBNP clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of NT-ProBNP in canine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to NT-ProBNP. A competitive inhibition reaction is launched between biotin labeled NT-ProBNP and unlabeled NT-ProBNP (Standards or samples) with the pre-coated antibody specific to NT-ProBNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NT-ProBNP in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the NT-ProBNP level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to NT-ProBNP. A competitive inhibition reaction is launched between biotin labeled NT-ProBNP and unlabeled NT-ProBNP (Standards or samples) with the pre-coated antibody specific to NT-ProBNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NT-ProBNP in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the NT-ProBNP level in the sample or standard.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Natriuretic peptides B
NCBI Official Synonym Full Names
natriuretic peptide B
NCBI Official Symbol
NPPB
NCBI Official Synonym Symbols
BNP
NCBI Protein Information
natriuretic peptides B
UniProt Protein Name
Natriuretic peptides B
UniProt Gene Name
NPPB
UniProt Synonym Gene Names
BNP-34; BNP-29
Uniprot Description
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 ().
Research Articles on NT-ProBNP
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Product Notes
The Canine NT-ProBNP nppb (Catalog #AAA2707907) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707907 CLIA Kit recognizes Canine NT-ProBNP. AAA Biotech's N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) can be used in a range of immunoassay formats including, but not limited to, Chemiluminescence Immunoassay (CLIA). Researchers should empirically determine the suitability of the NT-ProBNP nppb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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