Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
3260 results for " macrophages" - showing 550-600
Western Blot (WB)
(Western blot analysis of K562 cell lysate, using S100A12 Antibody. The lane on the left is treated with the antigen-specific peptide.)
S100A12, Polyclonal Antibody (Cat# AAA9607915)
Full Name
S100A12 Antibody
Gene Names
S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Figure. The binding activity of IL33 with IL1RL1.Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines. IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. IL-33 is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. Besides, Interleukin 1 Receptor Like Protein 1 (IL1RL1) has been identified as an interactor of IL33, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL33 and recombinant human IL1RL1. Briefly, IL33 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1RL1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL33pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL33and IL1RL1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 33 (IL33), Active Protein (Cat# AAA2105157)
Full Name
Active Interleukin 33 (IL33)
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$240/0.01 mg | $515/0.05 mg | $800/0.1 mg | $990/0.2 mg | $1,935/0.5 mg | $2,865/1 mg | $7,010/5 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215798)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216595)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Flow Cytometry (FC/FACS)
(Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(FITC Conjugated)(filled gray curve). Unstained macrophages (blank black curve) are used as control.)
F4/80, Monoclonal Antibody (Cat# AAA2558002)
Full Name
Anti-Mouse F4/80 Monoclonal Antibody (FITC Conjugated) [CI:A3-1]
Gene Names
Adgre1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Reactivity
Mouse
Applications
FC/FACS
Pricing
$100/0.05 mg | $120/0.1 mg | $140/0.2 mg | $85/0.025 mg | $615/5x0.2 mg
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212645))
CD200R, Monoclonal Antibody (Cat# AAA210910)
Full Name
RAT ANTI MOUSE CD200R:FITC
Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Applications
FC/FACS
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
( Western Blot using anti-CD52 antibody YTH 34.5-G2b (Campath-1G) Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. successfully detected CD52 in mouse spleen samples.)
CD52, Recombinant Antibody (Cat# AAA488135)
Full Name
Anti-CD52 [YTH 34.5-G2b (Campath-1G)]
Gene Names
CD52; CDW52
Reactivity
Human, Rhesus Monkey
Applications
EIA, IHC, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data #1
(Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR (MBS224569) followed by Goat anti Mouse IgG (H/L):FITC (MBS235142))
MHC CLASS II DR, Monoclonal Antibody (Cat# AAA224569)
Full Name
MOUSE ANTI BOVINE MHC CLASS II DR
Applications
FC/FACS
Pricing
$505/0.25 mg | $2,130/5x0.25 mg
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC (MBS211113). Permeabilised with Leucoperm)
MACROPHAGES/MONOCYTES, Monoclonal Antibody (Cat# AAA213181)
Full Name
MOUSE ANTI HUMAN MACROPHAGES
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
FC/FACS
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.)
CD169, Monoclonal Recombinant Antibody (Cat# AAA488383)
Full Name
Anti-CD169 [3D6.112 (Recombinant Version)]
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Applications
WB, IHC
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))
CD204, Monoclonal Antibody (Cat# AAA214936)
Full Name
RAT ANTI MOUSE CD204:Low Endotoxin
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
EIA, FC/FACS, FN, IP, WB
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)
TLR4, Polyclonal Antibody (Cat# AAA9610817)
Full Name
Phospho-TLR4 (Ser800) Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DC-SIGN using anti-DC-SIGN antibody (MBS1750548).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat spleen tissue lysates,Lane 6: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DC-SIGN antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DC-SIGN at approximately 46KD. The expected band size for DC-SIGN is at 46KD. )
DC-SIGN, Polyclonal Antibody (Cat# AAA1750548)
Full Name
Anti-DC-SIGN Picoband antibody
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power)
CD11b, Monoclonal Antibody (Cat# AAA216561)
Full Name
RAT ANTI MOUSE CD11b:Biotin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215793)
Full Name
RAT ANTI MOUSE DECTIN-1:FITC
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
Testing Data
(Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)
CD18, Monoclonal Antibody (Cat# AAA211045)
Full Name
RAT ANTI HUMAN CD18:FITC
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$415/100 Tests | $1,710/5x100 Tests
Flow Cytometry (FC)
(Mouse abdominal macrophages elicited by starch broth are stained with Anti-Mouse F4/80 Monoclonal Antibody(APC
Conjugated)(filled gray histogram). Unstained macrophages (blank black histogram) are used as control.)
F4/80, Monoclonal Antibody (Cat# AAA2553719)
Full Name
Anti-Mouse F4/80 Monoclonal Antibody (APC Conjugated)
Gene Names
Adgre1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Reactivity
Mouse
Applications
FC/FACS
Pricing
$105/100 Tests | $90/50 Tests | $175/500 Tests | $785/5x500 Tests
Immunohistochemistry (IHC)
(Anti-TLR5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
TLR5, Polyclonal Antibody (Cat# AAA240315)
Full Name
Rabbit Polyclonal to Human TLR5
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human, Mouse, Rat
Applications
IHC - Paraffin, WB
Purity
Immunoaffinity Purified
Pricing
$705/0.05 mg | $3,120/5x0.05 mg
Testing Data
MMP9, Polyclonal Antibody (Cat# AAA5312688)
Full Name
MMP9 Antibody
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Applications
WB, IHC
Purity
Affinity Chromatography
Pricing
$730/0.1 mg | $3,265/5x0.1 mg
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
SHIP2, Polyclonal Antibody (Cat# AAA9610473)
Full Name
Phospho-SHIP2 (Tyr886) Antibody
Gene Names
INPPL1; OPSMD; SHIP2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Kidney stained with C1QA Mouse Monoclonal Antibody (C1QA/2952).)
C1QA/Complement C1q A-Chain, Monoclonal Antibody (Cat# AAA4381293)
Full Name
C1QA/Complement C1q A-Chain
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36:Alexa Fluor ?647 (MBS217380A647))
CD36, Monoclonal Antibody (Cat# AAA217381)
Full Name
RAT ANTI MOUSE CD36:Low Endotoxin
Gene Names
Cd36; FAT; GPIV; Scarb3
Applications
FC/FACS, FN, IF, IP, WB
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data
(Staining of mouse peripheral blood lymphocytes Rat anti Mouse CD16/32: Alexa Fluor 488 (MBS212682A488))
CD16/CD32, Monoclonal Antibody (Cat# AAA212682)
Full Name
RAT ANTI MOUSE CD16/CD32
Gene Names
Fcgr2b; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
Applications
FC/FACS
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))
CD204, Monoclonal Antibody (Cat# AAA214939)
Full Name
RAT ANTI MOUSE CD204
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
EIA, FC/FACS, IF, IP, WB
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
MIP-1 alpha, Active Protein (Cat# AAA691838)
Full Name
Rat MIP-1 alpha
Gene Names
Ccl3; Scya3; MIP-1a
Reactivity
Rat
Purity
> 95% (SDS-PAGE; HPLC)
Pricing
$395/0.02 mg | $250/0.005 mg | $1,565/5x0.02 mg
Western Blot
(Western Blot analysis using CSF2 antibodyWestern Blot showing CSF2 antibody used against CSF2 (AA: 18-144)-hIgGFc transfected HEK293 cell lysate.)
CSF2, Monoclonal Antibody (Cat# AAA831778)
Full Name
CSF2 antibody
Gene Names
CSF2; GMCSF
Applications
EIA, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212645))
CD200R, Monoclonal Antibody (Cat# AAA215759)
Full Name
RAT ANTI MOUSE CD200R:RPE
Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Applications
FC/FACS
Pricing
$240/25 Tests | $930/5x25 Tests
Testing Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 (MBS216653), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
CD169, Monoclonal Antibody (Cat# AAA219361)
Full Name
RAT ANTI MOUSE CD169:Low Endotoxin
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Applications
FC/FACS, IF
Pricing
$830/0.5 mg | $3,580/5x0.5 mg
Activity Data
(Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFalpha, IL-1beta, IL-12, and IFNgamma made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1beta was determined in the supernatants by cytokine specific ELISA.Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1beta and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1beta as shown in Table 1.)
Interleukin 10 (IL10), Active Protein (Cat# AAA2086062)
Full Name
Active Interleukin 10 (IL10)
Gene Names
IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $525/0.05 mg | $815/0.1 mg | $1,010/0.2 mg | $1,975/0.5 mg | $2,925/1 mg | $7,155/5 mg
SDS-Page
(SDS-PAGE Analysis Purified ACTH Recombinant Mouse Monoclonal Antibody (rCLIP/1418). Confirmation of Purity and Integrity of Antibody.)
ACTH (Adrenocorticotrophic Hormone), Antibody (Cat# AAA4381182)
Full Name
ACTH (Adrenocorticotrophic Hormone) (C-Terminus)
Gene Names
POMC; LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2953).)
C1QA/Complement C1q A-Chain, Monoclonal Antibody (Cat# AAA4381294)
Full Name
C1QA/Complement C1q A-Chain
Reactivity
Human
Applications
WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE (MBS213862))
CD172a, Monoclonal Antibody (Cat# AAA213106)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, IP, WB
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD23-Monospecific Mouse Monoclonal Antibody (FCER2/3592).)
CD23, Monoclonal Antibody (Cat# AAA4380896)
Full Name
CD23 (Fc Epsilon RII)
Gene Names
FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Texas red conjugated Goat anti Mouse IgG antibody used for the detection of bound anti CD80 antibody by immunofluorescenceImage caption:Immunolabelling of Mphi by the monocyte/Mphi marker CD14 and the M1 Mphi marker CD80. Mphi were seeded at a density of 5.7×104 cells/cm2, with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 mug/ml LPS and 5 ng/ml IFNgamma for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mphi cultured alone and was not detectable on Mphi in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.BMC Cancer. 2010 Jul 17;10:375)
IgG, Polyclonal Antibody (Cat# AAA225563)
Full Name
Goat Anti Mouse IgG: FITC
Reactivity
Mouse
Applications
FC/FACS, IF
Purity
Purified Ig prepared by affinity chromatography on pooled mouse IgG covalently linked to agarose
Pricing
$250/1 mg | $975/5x1 mg
Testing Data
(Staining of Mouse peripheral blood platelets with Rat anti Mouse CD36: Alexa Fluor 647 (MBS217380A647))
CD36, Monoclonal Antibody (Cat# AAA219350)
Full Name
RAT ANTI MOUSE CD36:FITC
Gene Names
Cd36; FAT; GPIV; Scarb3
Applications
FC
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
(Western blot analysis of TCTP in lysates of HepG2 , using TCTP Antibody.)
TCTP, Polyclonal Antibody (Cat# AAA9611010)
Full Name
TCTP Antibody
Gene Names
TPT1; HRF; p02; p23; TCTP
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))
CD204, Monoclonal Antibody (Cat# AAA214938)
Full Name
RAT ANTI MOUSE CD204:FITC
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC (MBS211113). Permeabilised with Leucoperm)
MACROPHAGES/MONOCYTES, Monoclonal Antibody (Cat# AAA219286)
Full Name
MOUSE ANTI HUMAN MACROPHAGES:Low Endotoxin
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Applications
FC/FACS
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Published customer image: Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1beta antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1beta, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 um in length. These micrographs were taken of dermal tissue under 630x magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.)
Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA216593)
Full Name
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE
Applications
FC/FACS
Pricing
$550/100 Tests | $2,320/5x100 Tests
Immunohistochemistry (IHC)
(Sample Type: Human Lung TissueCA4 antibody - C-terminal region Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasm and membrane of alveolar macrophagesPrimary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)
CA4, Polyclonal Antibody (Cat# AAA3205833)
Full Name
CA4 antibody - C-terminal region
Gene Names
CA4; CAIV; Car4; RP17
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Mouse Monoclonal Antibody (C68/2709).)
CD68, Monoclonal Antibody (Cat# AAA4380749)
Full Name
CD68 (Macrophage Marker)
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data #1
(Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 (MBS224725) followed by rabbit F(ab')2 anti mouse IgG:FITC (MBS235160))
SIGLEC-5/SIGLEC-14, Monoclonal Antibody (Cat# AAA224725)
Full Name
MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14
Gene Names
SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Applications
EIA, FC/FACS, IF, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215790)
Full Name
RAT ANTI MOUSE DECTIN-1:Biotin
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$460/0.1 mg | $1,910/5x0.1 mg
RANTES, Active Protein (Cat# AAA553670)
Full Name
RANTES
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Applications
SDS-PAGE
Purity
>95%, as determined by SDS-PAGE and HPLC.
Pricing
$290/0.02 mg | $3,285/1 mg | $210/0.005 mg
Immunohistochemistry (IHC)
(Sample Type: Human KidneySample Type: Kidney)
CCL2, Polyclonal Antibody (Cat# AAA3224420)
Full Name
CCL2 antibody - middle region
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Dog, Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
CD209/DC-SIGN, Monoclonal Antibody (Cat# AAA4380585)
Full Name
CD209/DC-SIGN (Pathogen Receptor on Dendritic Cells)
Gene Names
CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
Reactivity
Human. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
TL1A, Monoclonal Recombinant Antibody (Cat# AAA488477)
Full Name
Anti-TL1A [TAN2-2]
Gene Names
Tnfsf15; Tl1; Tl1a; Vegi; Tnlg1b
Reactivity
Mouse
Applications
FC/FACS, IF, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE)
CD172a, Monoclonal Antibody (Cat# AAA213862)
Full Name
MOUSE ANTI RAT CD172a:RPE
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
$510/100 Tests | $2,140/5x100 Tests
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD137-Monospecific Mouse Monoclonal Antibody (4-1BB/3201).)
CD137/4-1BB/TNFRSF9, Monoclonal Antibody (Cat# AAA4381020)
Full Name
CD137/4-1BB/TNFRSF9
Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Reactivity
Human
Applications
EIA, IF, FC/FACS, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
