Mouse CD172a Monoclonal Antibody | anti-CD172a antibody

MOUSE ANTI RAT CD172a

Cat. Num. AAA213106

• Gene Names: Sirpa; Bit; Ptpns1; SHPS-1
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD172a; Monoclonal Antibody; MOUSE ANTI RAT CD172a; anti-CD172a antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ED9
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-CD172a antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/1000; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE (MBS213862))

Testing Data

(Published clone specific image: Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 (left) and ED9 (right) expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data

(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data

(Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Low power)

Testing Data

(Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Related Product Information for anti-CD172a antibody

Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRP&alpha, -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid %#126;56 kDa single pass typew 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons(UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OCX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).

NCBI and Uniprot Product Information

• NCBI GI #: 31543529
• NCBI GeneID: 25528
• NCBI Accession #: NP_037148.2
• NCBI GenBank Nucleotide #: NM_013016.2
• UniProt Accession #: P97710; O08951; O70426; Q9QWI5
• Molecular Weight: 55,691 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type substrate 1
• NCBI Official Synonym Full Names: signal-regulatory protein alpha
• NCBI Official Symbol: Sirpa
• NCBI Official Synonym Symbols: Bit; Ptpns1; SHPS-1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1; Brain immunoglobulin like protein with tyrosine - based activation motifs; CD172 antigen-like family member A; Protein tyrosine phosphatase non-receptor type substrate 1 (SHP substrate 1); Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1); brain Ig-like molecule with tyrosine-based activation motifs; inhibitory receptor SHPS-1; macrophage fusion receptor; macrophage membrane protein MFP150; signal-regulatory protein alpha-1; sirp-alpha-1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Gene Name: Sirpa
• UniProt Synonym Gene Names: Bit; Mfr; Ptpns1; Shps1; Sirp; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1
• UniProt Entry Name: SHPS1_RAT

NCBI Description

plays a role in macrophage fusion [RGD, Feb 2006]

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein phosphorylated amino acid binding; protein binding; SH3 domain binding

Biological Process: cell migration; cell-matrix adhesion; positive regulation of phagocytosis; actin filament organization; phagocytosis, recognition; cytoskeleton organization and biogenesis; hemopoietic progenitor cell differentiation; phagocytosis, engulfment