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Typical Testing Data/Standard Curve (for reference only) ()

Human Neuropeptide Y (NP-Y) ELISA Kit | NP-Y elisa kit

Human Neuropeptide Y (NP-Y) ELISA Kit

Gene Names
NPY; PYY4
Reactivity
Human
Synonyms
Neuropeptide Y (NP-Y); Human Neuropeptide Y (NP-Y) ELISA Kit; NP-Y elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
97
Samples
Human serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
1000 pg/mL-15.6 pg/mL
Sensitivity
Up to 5 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for NP-Y elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human NP-Y monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
10,851 Da
NCBI Official Full Name
neuropeptide Y, partial
NCBI Official Synonym Full Names
neuropeptide Y
NCBI Official Symbol
NPY
NCBI Official Synonym Symbols
PYY4
NCBI Protein Information
pro-neuropeptide Y; prepro-neuropeptide Y
UniProt Protein Name
Pro-neuropeptide Y
UniProt Gene Name
NPY
UniProt Synonym Gene Names
NPY; CPON
UniProt Entry Name
NPY_HUMAN

NCBI Description

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. [provided by RefSeq, Nov 2009]

Uniprot Description

NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 7p15.1

Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region

Molecular Function: G-protein coupled receptor activity; neuropeptide hormone activity; neuropeptide Y receptor binding; calcium channel regulator activity; receptor binding

Biological Process: blood circulation; behavior; adult feeding behavior; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; cell proliferation; neuropeptide signaling pathway; calcium ion transport; regulation of blood pressure; positive regulation of appetite; digestion; regulation of appetite; feeding behavior; cerebral cortex development; cell motility; neurite development; central nervous system neuron development

Research Articles on NP-Y

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Product Notes

The Human NP-Y npy (Catalog #AAA265603) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA265603 ELISA Kit recognizes Human NP-Y. It is sometimes possible for the material contained within the vial of "Neuropeptide Y (NP-Y), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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