Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$365/7 mL (RTU) | $235/0.1 mL (Concentrate) | $515/0.5 mL (Concentrate) | $845/1 mL (Concentrate) | $635/15 mL (RTU) | $290/3 mL (RTU) | $3,795/5x1 mL (Concentrate)
Western Blot (WB) (RNF180 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RNF180 antibody detected the RNF180 protein (arrow).)
Immunohistochemistry (IHC) (RNF180 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF180 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of extracts of rat brain, using PELI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, A549 cells and K562 cells, using UBE2D2 antibody.)
Western Blot (WB) (Western blotAll lanes: PELI1 antibody at 7ug/mlLane 1:HL-60 whole cell lysateLane 2:mouse liver tissueLane 3:THP-1 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 46kDaObserved band size: 46kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1493187 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1493187 at dilution 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate,Jurkat whole cell lysateAll lanes: PARK2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52,49,24,31,43,36,44,47 KDaObserved band size: 52 KDa)
Immunohistochemistry (IHC) (IHC image of MBS968850 diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (IHC image of MBS968850 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF) (Immunofluorescent analysis of PC3 cells using MBS968850 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using FBXW7 antibody (MBS9128018) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffinembedded mouse lung using FBXW7 antibody (MBS9128018) at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cells using FBXW7 antibody (MBS9128018) at dilution of 1:100.Blue: DAPI for nuclear staining.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffinembedded mouse kidney using FBXW7 antibody (MBS9128018) at dilution of 1:100 (40x lens).)
Western Blot (WB) (Western blotAll lanes: Ubiquitin-conjugating enzyme E2 D1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:NIH3T3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 17kDaObserved band size: 17kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7006023 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7006023 at dilution 1:100)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using VHL antibody (MBS125962) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat ovary using VHL antibody (MBS125962) at dilution of 1:200 (40x lens))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil using VHL antibody (MBS125962) at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse spleen using VHL antibody (MBS125962) at dilution of 1:200 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of U-251MG cells using VHL Polyclonal Antibody (MBS125962) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Western Blot (WB) (All lanes: E3 ubiquitin-protein ligase RBX1 antibody at 4ug/mlLane 1:Hela whole cell lysateLane 2:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 12kDaObserved band size: 12kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum using MBS1499945 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS1499945 at dilution 1:100)
Western Blot (WB) (Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Hela Cells; Lane3: Porcine Spleen Tissue; Lane4: Porcine Uterus Tissue.)
Western Blot (WB) (Western Blot: Sample: Recombinant UBE2I, Human.)
Immunohistochemistry (IHC) (FITC staining on IHC-P; Samples: Human Hela Cells.)
Knockout Validation (Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: UBE2I knockout Hela cell lysate; Predicted MW: 18kd Observed MW: 18kd Primary Ab: 2ug/ml Rabbit Anti-Human UBE2I Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Western Blot (WB) (Western BlotPositive WB detected in:Mouse brain tissueAll lanes: PDZRN3 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120,39,81 kDaObserved band size: 120,81,39 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7051551 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7051551 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (All lanes: E3 ubiquitin-protein ligase CBL-C antibody at 14ug/mlLane 1:A549 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53,48 kDaObserved band size: 52 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1491737 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue using MBS1491737 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: RNF128 antibody at 12ug/ml+Mouse liver tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 47,45 kDaObserved band size: 47 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells using MBS7000489 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: E3 ubiquitin-protein ligase MIB1 antibody at 2ug/ml+293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 111 kDaObserved band size: 111 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7005543 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells cells using MBS7005543 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysate,NIH/3T3 whole cell lysate,Mouse skeletal muscle tissueAll lanes: TRIM39 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60,57 KDaObserved band size: 45,50 KDa)
Immunohistochemistry (IHC) (IHC image of MBS7051340 diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7051340 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human heart tissue using MBS1495839 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using MBS7045468 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7045468 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7045468 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using BTRC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Western Blot (WB) (All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilutionLane 1: mouse thymus lysatesLane 2: rat spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western blot analysis of SOCS1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) with 293 cell followed by Alexa Fluor??488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blotAll lanes: XIAP antibody at 3ug/mlLane 1:HepG2 whole cell lysateLane 2:MCF-7 whole cell lysateLane 3:PC-3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 57kDaObserved band size: 57kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectal cancer using MBS1494489 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human melanoma using MBS1494489 at dilution 1:100)
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