Rabbit FBXW7 Polyclonal Antibody | anti-FBXW7 antibody

FBXW7 Polyclonal Antibody

Cat. Num. AAA9128018

• Gene Names: FBXW7; AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: FBXW7; Polyclonal Antibody; FBXW7 Polyclonal Antibody; AGO;CDC4;FBW6;FBW7;FBX30;FBXO30;FBXW6;hAgo;hCd c4;SEL-10;SEL10; anti-FBXW7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 589

• Applicable Applications for anti-FBXW7 antibody: Western Blot (WB), Immunohistochemsitry (IHC), Immunofluoresecence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1)
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FBXW7 antibody (MBS9128018) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded mouse lung using FBXW7 antibody (MBS9128018) at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using FBXW7 antibody (MBS9128018) at dilution of 1:100.Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded mouse kidney using FBXW7 antibody (MBS9128018) at dilution of 1:100 (40x lens).)

Related Product Information for anti-FBXW7 antibody

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 61743926
• NCBI GeneID: 55294
• NCBI Accession #: NP_001013433.1
• NCBI GenBank Nucleotide #: NM_001013415.1
• UniProt Accession #: Q969H0; Q68DR0; Q96A16; Q96LE0; Q96RI2; Q9NUX6; B7ZLP9
• Molecular Weight: Calculated MW: 66kDa/70kDa/79kDa; Observed MW: 80 kDa
• NCBI Official Full Name: F-box/WD repeat-containing protein 7 isoform 3
• NCBI Official Synonym Full Names: F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
• NCBI Official Symbol: FBXW7
• NCBI Official Synonym Symbols: AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10
• NCBI Protein Information: F-box/WD repeat-containing protein 7
• UniProt Protein Name: F-box/WD repeat-containing protein 7
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: FBXW7
• UniProt Synonym Gene Names: hAgo
• UniProt Entry Name: FBXW7_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

FBXW7: a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins including MYC, cyclin E, NOTCH1-released notch intracellular domain (NICD), JUN, and probably PSEN1. Recognizes and binds to phosphorylated target proteins. Other components of the SCF(FBXW7) complex are sCUL1, RBX1, and SKP1A. Defects in FBXW7 may be a cause of breast cancer. The homolog of Cdc4, one of the yeast genes that regulate sister chromatid cohesion. Down-regulation or disruption of FBXW7 resulted in abnormal sister chromatid cohesion in human cells. Four alternatively spliced isoforms have been described.

Protein type: Ubiquitin conjugating system; Nucleolus; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: nucleoplasm; Golgi apparatus; protein complex; endoplasmic reticulum; cytoplasm; nucleolus; SCF ubiquitin ligase complex

Molecular Function: protein binding, bridging; identical protein binding; cyclin binding; protein binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; protein stabilization; viral reproduction; sister chromatid cohesion; protein ubiquitination; lipid homeostasis; negative regulation of DNA endoreduplication; regulation of protein localization; regulation of transcription, DNA-dependent; positive regulation of epidermal growth factor receptor activity; vasculature development; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; negative regulation of Notch signaling pathway; vasculogenesis; response to DNA damage stimulus; lung development