Rabbit anti-Human, Mouse Guanylate Cyclase alpha-2, Soluble Polyclonal Antibody | anti-GUCY1A2 antibody

Guanylate Cyclase alpha-2, Soluble, NT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2)

Cat. Num. AAA629426

• Gene Names: GUCY1A2; GC-SA2; GUC1A2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: Guanylate Cyclase alpha-2; Soluble; Polyclonal Antibody; NT (Guanylate Cyclase Soluble Subunit alpha-2; Guanylate Cyclase 1 Soluble alpha 2; GCS-alpha-2; GC-SA2; GUC1A2; GUCSA2; GUCY1A2); Anti -Guanylate Cyclase alpha-2; anti-GUCY1A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GUCY1A2. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-GUCY1A2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:200; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human GUCY1A2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-GUCY1A2 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). GUCY1A2 (arrow) was detected using the purified Pab.)

Related Product Information for anti-GUCY1A2 antibody

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs.

Product Categories/Family for anti-GUCY1A2 antibody

Antibodies; Abs to Enzymes; Cyclase

NCBI and Uniprot Product Information

• NCBI GI #: 4504211
• NCBI GeneID: 2977
• NCBI Accession #: NP_000846.1
• NCBI GenBank Nucleotide #: NM_000855.1
• UniProt Accession #: P33402; A1L4C4
• Molecular Weight: 81,750 Da
• NCBI Official Full Name: guanylate cyclase soluble subunit alpha-2
• NCBI Official Synonym Full Names: guanylate cyclase 1, soluble, alpha 2
• NCBI Official Symbol: GUCY1A2
• NCBI Official Synonym Symbols: GC-SA2; GUC1A2
• NCBI Protein Information: guanylate cyclase soluble subunit alpha-2; GCS-alpha-2; OTTHUMP00000232532; OTTHUMP00000232533; OTTHUMP00000232534
• UniProt Protein Name: Guanylate cyclase soluble subunit alpha-2
• Protein Family: Guanylate cyclase soluble
• UniProt Gene Name: GUCY1A2
• UniProt Synonym Gene Names: GUC1A2; GUCSA2
• UniProt Entry Name: GCYA2_HUMAN

NCBI Description

Soluble guanylyl (or guanylate) cyclases are heterodimeric enzymes consisting of an alpha subunit, such as alpha-2 (GUCY1A2), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), which are activated by nitric oxide (NO) and which catalyze conversion of GTP to 3-prime, 5-prime-cyclic GMP and pyrophosphate (Harteneck et al., 1991 [PubMed 1683630]).[supplied by OMIM]

Uniprot Description

GUCY1A2: Has guanylyl cyclase on binding to the beta-1 subunit. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Nucleotide Metabolism - purine; EC 4.6.1.2; Guanylyl cyclase

Chromosomal Location of Human Ortholog: 11q21-q22

Cellular Component: cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; heme binding

Biological Process: cGMP biosynthetic process; positive regulation of cGMP biosynthetic process; blood coagulation; signal transduction