Human Probable E3 ubiquitin-protein ligase makorin-3 ELISA Kit | MKRN3 elisa kit

Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit

Cat. Num. AAA281111

• Gene Names: MKRN3; CPPB2; D15S9; RNF63; ZFP127; ZNF127
• Reactivity: Human
• Synonyms: Probable E3 ubiquitin-protein ligase makorin-3; Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit; MKRN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MKRN3. No significant cross-reactivity or interference between Human MKRN3 and analogues was observed.
• Sequence Length: 507

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/mL - 2000 pg/mL
• Sensitivity: 7.8 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MKRN3 elisa kit

Intended Uses: For the quantitative detection of Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) concentration in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MKRN3 in Human serum, plasma, tissue homogenates, cell lysates. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5032243
• NCBI GeneID: 7681
• NCBI Accession #: NP_005655.1
• NCBI GenBank Nucleotide #: NM_005664.3
• UniProt Accession #: Q13064
• Molecular Weight: 55,645 Da
• NCBI Official Full Name: probable E3 ubiquitin-protein ligase makorin-3
• NCBI Official Synonym Full Names: makorin ring finger protein 3
• NCBI Official Symbol: MKRN3
• NCBI Official Synonym Symbols: CPPB2; D15S9; RNF63; ZFP127; ZNF127
• NCBI Protein Information: probable E3 ubiquitin-protein ligase makorin-3
• UniProt Protein Name: Probable E3 ubiquitin-protein ligase makorin-3
• Protein Family: Probable E3 ubiquitin-protein ligase makorin
• UniProt Gene Name: MKRN3
• UniProt Synonym Gene Names: D15S9; RNF63; ZNF127

NCBI Description

The protein encoded by this gene contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. This gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MKRN3: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 15q11.2

Cellular Component: intracellular ribonucleoprotein complex

Molecular Function: identical protein binding; metal ion binding; protein binding; transferase activity

Biological Process: protein ubiquitination

Disease: Prader-willi Syndrome; Precocious Puberty, Central, 2