Human Probable E3 ubiquitin-protein ligase makorin-3 ELISA Kit | MKRN3 elisa kit
Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit
Gene Names
MKRN3; CPPB2; D15S9; RNF63; ZFP127; ZNF127
Reactivity
Human
Synonyms
Probable E3 ubiquitin-protein ligase makorin-3; Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit; MKRN3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MKRN3. No significant cross-reactivity or interference between Human MKRN3 and analogues was observed.
Sequence Length
507
Samples
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/mL - 2000 pg/mL
Sensitivity
7.8 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MKRN3 elisa kit
Intended Uses: For the quantitative detection of Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) concentration in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MKRN3 in Human serum, plasma, tissue homogenates, cell lysates. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MKRN3 in Human serum, plasma, tissue homogenates, cell lysates. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,645 Da
NCBI Official Full Name
probable E3 ubiquitin-protein ligase makorin-3
NCBI Official Synonym Full Names
makorin ring finger protein 3
NCBI Official Symbol
MKRN3
NCBI Official Synonym Symbols
CPPB2; D15S9; RNF63; ZFP127; ZNF127
NCBI Protein Information
probable E3 ubiquitin-protein ligase makorin-3
UniProt Protein Name
Probable E3 ubiquitin-protein ligase makorin-3
Protein Family
UniProt Gene Name
MKRN3
UniProt Synonym Gene Names
D15S9; RNF63; ZNF127
NCBI Description
The protein encoded by this gene contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. This gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MKRN3: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 15q11.2
Cellular Component: intracellular ribonucleoprotein complex
Molecular Function: identical protein binding; metal ion binding; protein binding; transferase activity
Biological Process: protein ubiquitination
Disease: Prader-willi Syndrome; Precocious Puberty, Central, 2
Research Articles on MKRN3
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Product Notes
The Human MKRN3 mkrn3 (Catalog #AAA281111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281111 ELISA Kit recognizes Human MKRN3. It is sometimes possible for the material contained within the vial of "Probable E3 ubiquitin-protein ligase makorin-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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