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Western Blot (WB) (RNF180 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RNF180 antibody detected the RNF180 protein (arrow).)

Rabbit anti-Human RNF180 Polyclonal Antibody | anti-RNF180 antibody

RNF180 Antibody (C-term)

Gene Names
RNF180; RINES
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RNF180; Polyclonal Antibody; RNF180 Antibody (C-term); E3 ubiquitin-protein ligase RNF180; 632-; RING finger protein 180; anti-RNF180 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RNF180 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human RNF180.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
401-430
Sequence Length
592
Applicable Applications for anti-RNF180 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RNF180 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RNF180 antibody detected the RNF180 protein (arrow).)

Western Blot (WB) (RNF180 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RNF180 antibody detected the RNF180 protein (arrow).)

Immunohistochemistry (IHC)

(RNF180 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF180 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RNF180 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF180 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-RNF180 antibody
E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2 (By similarity).
Product Categories/Family for anti-RNF180 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68254
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF180 isoform 1
NCBI Official Synonym Full Names
ring finger protein 180
NCBI Official Symbol
RNF180
NCBI Official Synonym Symbols
RINES
NCBI Protein Information
E3 ubiquitin-protein ligase RNF180
UniProt Protein Name
E3 ubiquitin-protein ligase RNF180
UniProt Gene Name
RNF180
UniProt Entry Name
RN180_HUMAN

Uniprot Description

RNF180: E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: intrinsic to endoplasmic reticulum membrane; integral to membrane; nuclear envelope

Molecular Function: zinc ion binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein polyubiquitination; positive regulation of protein ubiquitination; regulation of catalytic activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; adult behavior; norepinephrine metabolic process; serotonin metabolic process

Research Articles on RNF180

Similar Products

Product Notes

The RNF180 rnf180 (Catalog #AAA9202940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 401-430. The RNF180 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF180 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the RNF180 rnf180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF180, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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