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Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Nitric Oxide Synthase Interacting Protein (NOSIP) Polyclonal Antibody | anti-NOSIP antibody
Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP)
Gene Names
NOSIP; CGI-25
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Nitric Oxide Synthase Interacting Protein (NOSIP); Polyclonal Antibody; Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP); anti-NOSIP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NOSIP. It has been selected for its ability to recognize NOSIP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC VEKLIRKDMV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC VEKLIRKDMV
Sequence Length
154
Applicable Applications for anti-NOSIP antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NOSIP (Gly62~Ser295) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041120
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: NOSIP knockout 293T cell lysate; Predicted MW: 33kd Observed MW: 35kd Primary Ab: 5ug/ml Rabbit Anti-Human NOSIP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: NOSIP knockout 293T cell lysate; Predicted MW: 33kd Observed MW: 35kd Primary Ab: 5ug/ml Rabbit Anti-Human NOSIP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Product Categories/Family for anti-NOSIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,172 Da
NCBI Official Full Name
nitric oxide synthase-interacting protein
NCBI Official Synonym Full Names
nitric oxide synthase interacting protein
NCBI Official Symbol
NOSIP
NCBI Official Synonym Symbols
CGI-25
NCBI Protein Information
nitric oxide synthase-interacting protein
UniProt Protein Name
Nitric oxide synthase-interacting protein
Protein Family
UniProt Gene Name
NOSIP
NCBI Description
The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]
Uniprot Description
NOSIP: Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity. Belongs to the NOSIP family.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cytoplasm; cytosol; Golgi membrane; nucleus
Molecular Function: protein binding; RNA binding
Biological Process: multicellular organism development; negative regulation of catalytic activity; negative regulation of nitric-oxide synthase activity; protein ubiquitination; regulation of nitric-oxide synthase activity
Research Articles on NOSIP
Similar Products
Product Notes
The NOSIP nosip (Catalog #AAA2014375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nitric Oxide Synthase Interacting Protein (NOSIP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase Interacting Protein (NOSIP) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NOSIP nosip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC VEKLIRKDMV. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase Interacting Protein (NOSIP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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