Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$350/0.2 mL | $570/0.1 mL (Biotin) | $1,515/5x0.2 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB) (Peptide-+ Western blot analysis of extracts from Jurkat cells, using 14-3-3 eta antibody. Immunofluorescence analysis of HeLa cells, using 14-3-3 eta antibody.)
Western Blot (WB) (Figure 1: Western blot analysis using A1BG mouse mAb against A1BG-hIgGFc transfected HEK293 (3)cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human Kidney tissues using anti-A1BG mouse mAb)
Testing Data (DsbA antibody (MBS850200) at 1/3000 dilution interacts with recombinant DsbA protein. Predicted band size: 24KDa. Observed band size: 24KDa.)
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, A549 cells and K562 cells, using UBE2D2 antibody.)
Flow Cytometry (FC/FACS) (Figure 2: Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).)
Immunohistochemistry (IHC) (Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)
Testing Data (Western blot detection of LCK in Jurkat&Ramos cell lysates and using LCK antibody (1:1000 diluted). Predicted band size: 58KDa Observed band size: 58KDa.)
Western Blot (WB) (Western blotAll lanes: Protein argonaute-2 antibody at 2ug/mlLane 1: A549 whole cell lysateLane 2: Jurkats whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Raw264.7 whole cell lysateLane 6: K562 whole cell lysateLane 7: Mouse liver tissueLane 8: Mouse kidney tissueGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 98,94 kDaObserved band size: 90 kDa)
Immunohistochemistry (IHC) (IHC image of MBS1493111 diluted at 1:1200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Chromatin Immunoprecipitation (ChIP) (Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-AgO2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the SLC1A5 promoter.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS1493111 at dilution of 1:100)
Immunohistochemistry (IHC) (IHC image of MBS1493111 diluted at 1:1200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Western Blot (WB) (Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
Immunofluorescence (IF) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X)
Immunofluorescence (IF) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549 (upper, untreated or Hydroxyurea-treated) and Hela (lower, untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
Western Blot (WB) (Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells, using Histone H2A.X (Phospho-Ser139) mouse mAb (1:1000 diluted) (upper) or beta-Actin Mouse mAb (200068-8F10) (lower).Predicted band size:15KDa.Observed band size:15KDa.)
Western Blot (WB) (Western blotAll lanes: TROVE2SS-A/Ro ribonucleoprotein antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 61,24,59,60 kDaObserved band size: 61 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kiney using MBS7002184 at dilution of 1:20)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using MBS7002184 at dilution of 1:20)
Western Blot (WB) (Western BlotPositive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, PC3 whole cell lysate, Mouse heart tissue,Mouse spleen tissue,Mouse lung tissueAll lanes: TROVE2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 61,24,59,6 kDaobserved band size: 61 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7002184 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7002184 at a dilution of 1:100 )
Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,Rat kidney tissueAll lanes: TROVE2 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,24,59,60 KDaObserved band size: 61 KDa)
Immunofluorescence (IF) (Immunofluorescent analysis of PC3 cells using MBS7002184 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung tissue using MBS7002184 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: Mouse brain tissueAll lanes: SPDEF antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38,36 kDaObserved band size: 38 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7049204 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7049204 at dilution of 1:100)
Western Blot (WB) (Figure 1: Western blot analysis using BMPR2 mouse mAb against Hela (1), A431 (2), NIH/3T3 (3), Cos7 (4) and PC-12 (5) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded muscle tissues (left) and kidney cancer tissues (right) using BMPR2 mouse mAb with DAB staining.)
Western Blot (WB) (All lanes: RNA-binding protein FUS antibody at 2ug/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 60kDaObserved band size: 60kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS7003625 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7003625 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: ENTPD5 antibody at 4ug/mlLane 1:mouse liver tissueLane 2:mouse kidney tissueLane 3:mouse gonad tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine using MBS1492677 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS1492677 at dilution 1:100)
Immunoprecipitation (IP) (Immunoprecipitating ENTPD5 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1492677 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1492677(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysateAll lanes: ATF4 antibody at 1.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39 KDaObserved band size: 50 KDa)
Immunohistochemistry (IHC) (IHC image of CSB-RA002272A0HU diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF) (Immunofluorescence staining of HepG2 cells with CSB-RA002272A0HU at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP) (Immunoprecipitating ATF4 in Hela whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA002272A0HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA002272A0HU (3ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
Flow Cytometry (FC/FACS) (Overlay histogram showing Hela cells stained with CSB-RA002272A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Western Blot (WB) (Western blot detection of S6 Ribosomal Protein in Ramos, Jurkat, C6, 3T3 and Hela cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-S6 Ribosomal Protein mouse mAb (dilution 1:200).)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using S6 Ribosomal Protein Mouse mAb.)
Western Blot (WB) (Western blot detection of S6 Ribosomal Protein in CHO-K1, Raji, Raw264.7 and COS7 cell lysates using S6 Ribosomal Protein mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.)
Western Blot (WB) (All lanes: 39S ribosomal protein L19, mitochondrial antibody at 2ug/mlLane 1: MCF-7 whole cell lysateLane 2: mouse liver tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 34kDaObserved band size: 34kDaImmunohistochemistry)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human placenta using MBS7001166at dilution of 1:50)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7001166 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
Immunofluorescence (IF) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
Immunofluorescence (IF) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549 (upper, untreated or Hydroxyurea-treated) and Hela (lower, untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
Western Blot (WB) (Figure 1. Western blot analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).)