Rabbit anti-Human, Mouse SPDEF Polyclonal Antibody | anti-SPDEF antibody

SPDEF Antibody

Cat. Num. AAA7049204

• Gene Names: SPDEF; PDEF; bA375E1.3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: SPDEF; Polyclonal Antibody; SPDEF Antibody; SAM pointed domain-containing Ets transcription factor (Prostate epithelium specific Ets transcription factor) (Prostate-specific Ets) (Prostate-derived Ets factor); PDEF PSE; anti-SPDEF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-SPDEF antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB:1:1000-1:5000; IHC:1:20-1:200
• Immunogen: Recombinant Human SAM pointed domain-containing Ets transcription factor protein (145-247AA)
• Immunogen Species: Homo sapiens (Human)
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Research Area: Epigenetics and Nuclear Signaling
• Target Names: SPDEF
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: SPDEF antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38,36 kDaObserved band size: 38 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7049204 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7049204 at dilution of 1:100)

Related Product Information for anti-SPDEF antibody

May function as an androgen-independent trans activator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 356582513
• NCBI GeneID: 25803
• NCBI Accession #: NP_001239223.1
• NCBI GenBank Nucleotide #: NM_001252294.1
• UniProt Accession #: O95238; B4DWH8; F5H778
• NCBI Official Full Name: SAM pointed domain-containing Ets transcription factor isoform 2
• NCBI Official Synonym Full Names: SAM pointed domain containing ETS transcription factor
• NCBI Official Symbol: SPDEF
• NCBI Official Synonym Symbols: PDEF; bA375E1.3
• NCBI Protein Information: SAM pointed domain-containing Ets transcription factor
• UniProt Protein Name: SAM pointed domain-containing Ets transcription factor
• Protein Family: SAM pointed domain-containing Ets transcription factor
• UniProt Gene Name: SPDEF
• UniProt Synonym Gene Names: PDEF; PSE; Prostate-specific Ets
• UniProt Entry Name: SPDEF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

SPDEF: May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. Belongs to the ETS family.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleus

Biological Process: cell differentiation; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis