Typical Testing Data/Standard Curve (for reference only)
DNA Damage (8-OHdG) ELISA Kit | 8-OHdG elisa kit
DNA Damage (8-OHdG) ELISA Kit
Reactivity
Species Independent
Synonyms
DNA Damage (8-OHdG); DNA Damage (8-OHdG) ELISA Kit; Colorimetric detection of 8-hydroxy-2-deoxy Guanosine ; 8-OH-dG ELISA Kit; 8OHG ELISA Kit; 80G ELISA Kit; 8 hydroxyguanine ELISA Kit; 8-OHdG ELISA Kit; DNA Damage ELISA Kit; 8-OHdG elisa kit
Reactivity
Species Independent
Application Notes
Detection Range
0.94-60 ng/mL
Sensitivity
0.59 ng/mL
Preparation and Storage
Store at 4 degree C and -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 8-OHdG elisa kit
Background/Introduction: 8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA (see Figure 1, on page 7) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress.1-4 Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.5-9 In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/ml as determined by LC-MS.10-11
Product Categories/Family for 8-OHdG elisa kit
References
1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 116-120. 2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34. 3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids. 4. Lin H., et al. (2004) Biochem J. 380: 541-548. 5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666. 6. Lee J., et al. (2005) Hypertension 45: 986-990. 7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152. 8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75. 9. Kuo H., et al. (2007) Mutat Res. 631:62-68. 10. Shen J., et al. (2007) Cancer 109: 574-580. 11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636. 12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110. 13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236. 14. Floyd R.A. (1990) FASEB J 4: 2587-2597.
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Product Notes
The Species Independent 8-OHdG (Catalog #AAA808265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA808265 ELISA Kit recognizes Species Independent 8-OHdG. Download the calculation worksheet. Researchers should empirically determine the suitability of the 8-OHdG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Damage (8-OHdG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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