Human 4-1BB-R CLIA Kit | 4-1BB-R clia kit

4-1BB-R (Human) LumiAb ELISA Kit

Cat. Num. AAA9510755

• Gene Names: TNFRSF9; ILA; 4-1BB; CD137; CDw137
• Reactivity: Human
• Synonyms: 4-1BB-R; 4-1BB-R (Human) LumiAb ELISA Kit; Human 4-1BB-R; Tumor necrosis factor receptor superfamily member 9; 4-1BB ligand receptor T-cell; antigen 4-1BB homolog; T-cell antigen ILA; CD137 antigen; CDw137; ILA; 4-1BB; MGC2172; 4-1BBR; TNFRSF9; 4-1BB-R clia kit

• Reactivity: Human
• Specificity: The LumiAb, Human 4-1BBR ELISA is capable of recognizing both recombinant and naturally produced Human 4-1BBR proteins.; The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: 4-1BBL, AITRL, BAFF, BAFF Receptor, BCMA, CD40 Ligand/TRAP, Fas Receptor, LIGHT, OPG, sOX40L, sRANK (Receptor), sRANKL, TACI, TL-1A, TNF-alpha, TNF-beta, sTNF-Receptor Type I, sTNF-Receptor Type II, sTRAIL/Apo2L, sTRAIL Receptor-1, sTRAIL Receptor-2, TWEAK, TWEAK Receptor
• Sequence Length: 255

• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for 4-1BB-R clia kit

Principle of the Assay: The Inosine Assay Kit measures total inosine within biological samples. Inosine is converted into hypoxanthine by purine nucleoside phosphorylase (PNP). Then hypoxanthine is converted to xanthine and hydrogen peroxide by xanthine oxidase (XO). The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of inosine standard within the 96-well microtiter plate format. Samples and standards are incubated for 15 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1)!!Background/Introduction: Inosine is a nucleoside that is created when a ribose ring attaches to hypoxanthine through a beta-N9-glycosidic bond. In the DNA synthesis pathway, adenine is first modified to form adenosine or inosine monophosphate (IMP) Next, either form is converted into inosine which can form base pairs with adenine (A), cytosine (C), and uracil (U). Inosine is most often found in tRNAs and is important for faithful translation of the genetic code in wobble base pairs. A better understanding of inosine metabolism has led to immunotherapy advances in recent years. Inosine monophosphate is oxidized by inosine monophosphate dehydrogenase to create xanthosine monophosphate, an important precursor in purine metabolism. Mycophenolate mofetil is a drug that acts as an inhibitor of inosine monophosphate dehydrogenase and is used in the treatment of a number of autoimmune diseases including granulomatosis with polyangiitis. In addition inosine has been demonstrated to have neuroprotective properties. It has been suggested for administration in both spinal cord injury and after stroke since it enhances the rewiring of axonal connections. Inosine may also benefit multiple sclerosis (MS) patients since ingestion leads to conversion to uric acid that is thought to be a natural antioxidant and a peroxynitrite scavenger. Inosine treatment of Parkinson's disease patients has been shown to slow progression of the disease in clinical trials. The Inosine Assay Kit is a simple fluorometric assay that measures the amount of total inosine present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, inosine standards, and unknown samples. Sample inosine concentrations are determined by comparison with a known inosine standard. The kit has a detection sensitivity limit of 300 nM inosine.

Product Categories/Family for 4-1BB-R clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 5730095
• NCBI GeneID: 3604
• NCBI Accession #: NP_001552.2
• NCBI GenBank Nucleotide #: NM_001561.5
• UniProt Accession #: Q07011
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 9
• NCBI Official Synonym Full Names: TNF receptor superfamily member 9
• NCBI Official Symbol: TNFRSF9
• NCBI Official Synonym Symbols: ILA; 4-1BB; CD137; CDw137
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 9
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 9
• UniProt Gene Name: TNFRSF9
• UniProt Synonym Gene Names: CD137; ILA
• UniProt Entry Name: TNR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF9: Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: cytokine binding; receptor activity

Biological Process: negative regulation of cell proliferation; apoptosis