Rabbit anti-Human, Mouse Ectonucleoside triphosphate diphosphohydrolase 5 Polyclonal Antibody | anti-ENTPD5 antibody

Rabbit anti-human Ectonucleoside triphosphate diphosphohydrolase 5 polyclonal Antibody(ENTPD5)

Cat. Num. AAA1492677

• Gene Names: ENTPD5; PCPH; CD39L4; NTPDase-5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: Ectonucleoside triphosphate diphosphohydrolase 5; Polyclonal Antibody; Rabbit anti-human Ectonucleoside triphosphate diphosphohydrolase 5 polyclonal Antibody(ENTPD5); NTPDase 5; CD39 antigen-like 4; ER-UDPase; Guanosine-diphosphatase ENTPD5; GDPase ENTPD5; Nucleoside diphosphatase; Uridine-diphosphatase ENTPD5; UDPase ENTPD5; ENTPD5; CD39L4; PCPH; anti-ENTPD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 428

• Applicable Applications for anti-ENTPD5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Ectonucleoside triphosphate diphosphohydrolase 5 protein (149-428aa)
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: ENTPD5 antibody at 4ug/mlLane 1:mouse liver tissueLane 2:mouse kidney tissueLane 3:mouse gonad tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine using MBS1492677 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1492677 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating ENTPD5 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1492677 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1492677(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))

Related Product Information for anti-ENTPD5 antibody

Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 4557427
• NCBI GeneID: 957
• NCBI Accession #: NP_001240.1
• NCBI GenBank Nucleotide #: NM_001249.2
• UniProt Accession #: O75356; Q96RX0; A1L4C5
• Molecular Weight: 47,517 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 5
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 5
• NCBI Official Symbol: ENTPD5
• NCBI Official Synonym Symbols: PCPH; CD39L4; NTPDase-5
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 5
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 5
• Protein Family: Ectonucleoside triphosphate diphosphohydrolase
• UniProt Gene Name: ENTPD5
• UniProt Synonym Gene Names: CD39L4; PCPH; NTPDase 5; GDPase ENTPD5; UDPase ENTPD5
• UniProt Entry Name: ENTP5_HUMAN

NCBI Description

The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases. [provided by RefSeq, Jan 2009]

Uniprot Description

ENTPD5: Uridine diphosphatase (UDPase) that promotes protein N- glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. Belongs to the GDA1/CD39 NTPase family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.6.1.42; Oncoprotein; Hydrolase; Nucleotide Metabolism - purine; EC 3.6.1.6

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: endoplasmic reticulum

Molecular Function: guanosine-diphosphatase activity; protein binding; uridine-diphosphatase activity

Biological Process: 'de novo' posttranslational protein folding; ATP metabolic process; cell growth; cell proliferation; positive regulation of glycolysis; protein amino acid N-linked glycosylation; regulation of phosphoinositide 3-kinase cascade